Human Disease Model Report: cancer, germline stem cell models

General Information

Name

cancer, germline stem cell models

FlyBase ID

FBhh0000768

Disease Ontology Term

germ cell cancer

Parent Disease

OMIM

Overview

Drosophila germline stem cells (GSCs) provide accessible model systems to study stem cell behavior, including the roles of stem cell competition, niche maintenance, and niche occupancy.

A screen of aged Drosophila males has shown development of spontaneous tumors in the testis and gut; the incidence of these tumors increases with age. In the testis, loss of activity of the Drosophila gene Madm (orthologous to the human NRBP1 and NRBP2 genes) or constitutive activity of Dmel\hop (orthologous to human JAK genes) have been found to promote GSC tumor formation.

In ovaries, loss-of-function mutations of bam, which encodes a translation repressor, have been used to produce tumor-like stem cells. The role of autophagy has been studied in this system. Reduced function of Sxl or other genes involved in RNA processing of bam gives rise to the GSC tumor phenotype. Expression of bam in the cystoblast requires cell–cell communications elicited by the bone morphogenetic proteins (BMPs) from the somatic cells in the germarium; disruption of BMP signaling (FBgg00010860) results in ovarian germline tumor formation.

Drosophila adult stem cells have been found to be resistant to radiation- or chemical-induced apoptosis, thus providing a means to investigate mechanisms of resistance of stem cell tumors to various anticancer therapies. In a study using ovary germline stem cells, irradiation results in activation of a specific receptor tyrosine kinase (Dmel\Tie), which then results in upregulation of the microRNA mir-ban and the repression of apoptosis.

[updated Feb. 2022 by FlyBase; FBrf0222196]

Disease Summary Information

Disease Summary: cancer, germline stem cell models

OMIM report

Human gene(s) implicated

Symptoms and phenotype

Genetics

Cellular phenotype and pathology

Molecular information

External links

Disease synonyms

Related Diseases

Related human health report(s)

azoospermia and testicular germ cell cancer (postulated), FOXN3-related

myeloproliferative disorders, JAK2-related

Ortholog Information

Human gene(s) in FlyBase

Other mammalian ortholog(s) used

D. melanogaster Gene Information (4)

Dmel\Madm

Gene Snapshot

Contributions welcome

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

PSEUK-U

Comments on ortholog(s)

Moderate- to high-scoring ortholog of human NRBP1 and NRBP2 (1 Drosophila to 2 human). Dmel\Madm shares 49-50% identity and 64-66% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\hop

Gene Snapshot

hopscotch (hop) encodes a non-receptor tyrosine kinase for interleukin-like ligands (encoded by upd1, upd2, and upd3) and functions in the JAK-STAT signaling pathway. It is involved in embryonic segmentation, cell proliferation, and cell migration. [Date last reviewed: 2019-03-07]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Moderate-scoring ortholog of human JAK1 and JAK2; low-to-moderate-scoring ortholog of human TYK2 and JAK3 (1 Drosophila to 4 human); additional lower scoring orthologs exist in humans. Dmel\hop shares 24% identity and 39-41% similarity with JAK1 and JAK2.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\bam

Gene Snapshot

bag of marbles (bam) encodes a protein involved in gametogenesis that is associated with the fusome, a germ cell-specific organelle. It contributes to the fate determination of germline stem cells, in which bam is negatively regulated by the BMP signaling pathway. [Date last reviewed: 2019-03-07]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

No gene orthologous to Dmel\bam has been identified in human.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\mir-ban

Gene Snapshot

Contributions welcome

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

5' seed sequence homologous to that of human MIR450B-3p (FBrf0242402).

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Other Genes Used: Viral, Bacterial, Synthetic (0)

Summary of Physical Interactions (67 groups)

Madm

protein-protein

Interacting group

Assay

References

Madm - bun

two hybrid, anti tag coimmunoprecipitation, western blot, affinity technology, Identification by mass spectrometry

(Gluderer et al., 2010)

hop

RNA-protein

Interacting group

Assay

References

hop - mahe

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Maurya et al., 2021)

protein-protein

Interacting group

Assay

References

hop - CG15717

experimental knowledge based

(Guruharsha et al., 2011)

hop - Dera

experimental knowledge based

(Guruharsha et al., 2011)

hop - dome

enzymatic study, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Shen et al., 2022, Fisher et al., 2016, Kallio et al., 2010)

hop - et

anti tag coimmunoprecipitation, anti tag western blot

(Fisher et al., 2016, Kallio et al., 2010)

hop - eya

anti tag coimmunoprecipitation, anti tag western blot

(Hoi et al., 2016)

hop - Rel

anti tag coimmunoprecipitation, anti tag western blot

(Shen et al., 2022)

hop - Socs36E

anti tag coimmunoprecipitation, anti tag western blot

(Hoi et al., 2016, Stec et al., 2013)

hop - Socs44A

anti tag coimmunoprecipitation, anti tag western blot

(Hoi et al., 2016)

hop - Stat92E

anti tag coimmunoprecipitation, anti tag western blot

(Hoi et al., 2016)

bam

RNA-protein

Interacting group

Assay

References

bam - aub

iclip, partial RNA sequence identification

(Ma et al., 2017)

bam - how

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Monk et al., 2011, Monk et al., 2010)

bam - Mad

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Malik et al., 2019)

bam - mei-P26

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, two hybrid, bimolecular fluorescence complementation, fluorescence microscopy, molecular sieving, pull down

(Malik et al., 2017, Li et al., 2013, Insco et al., 2012)

bam - Myc

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Malik, 2021)

bam - nanos

anti tag coimmunoprecipitation, western blot, quantitative reverse transcription pcr

(Malik et al., 2020, Kim et al., 2010)

bam - Rbp9

nucleic acid uv cross-linking assay, autoradiography

(Kim-Ha et al., 1999)

bam - Su(var)205

clip, reverse transcription pcr

(Casale et al., 2019)

RNA-RNA

Interacting group

Assay

References

bam - mir-7

anti tag western blot, fluorescence technology

(Pek et al., 2009)

protein-protein

Interacting group

Assay

References

bam - AGO1

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Malik, 2021)

bam - bgcn

pull down, anti tag western blot, anti tag coimmunoprecipitation, two hybrid, three hybrid, molecular sieving, western blot, bimolecular fluorescence complementation, fluorescence microscopy

(Shan et al., 2017, Flores et al., 2015, Chen et al., 2014, Pan et al., 2014, Li et al., 2013, Insco et al., 2012, Kim et al., 2010, Li et al., 2009, Shen et al., 2009)

bam - brat

anti tag coimmunoprecipitation, anti tag western blot, two hybrid, bimolecular fluorescence complementation, fluorescence microscopy, western blot

(Malik, 2021, Malik et al., 2017)

bam - CG11700

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry

(Ji et al., 2017)

bam - CSN4

two hybrid, anti tag coimmunoprecipitation, anti tag western blot, western blot

(Pan et al., 2014)

bam - Cul2

anti tag coimmunoprecipitation, anti tag western blot

(Cai et al., 2023)

bam - CycA

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Ji et al., 2017)

bam - eIF4A

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Shen et al., 2009)

bam - mei-P26

(Malik et al., 2017, Li et al., 2013, Insco et al., 2012)

bam - nanos

anti tag coimmunoprecipitation, western blot, quantitative reverse transcription pcr

(Malik et al., 2020, Kim et al., 2010)

bam - Not1

pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot

(Sgromo et al., 2018)

bam - Not3

anti tag coimmunoprecipitation, anti tag western blot

(Sgromo et al., 2018)

bam - otu

anti tag coimmunoprecipitation, anti tag western blot, pull down, Identification by mass spectrometry

(Ji et al., 2019, Ji et al., 2017)

bam - pum

bimolecular fluorescence complementation, fluorescence microscopy, anti tag coimmunoprecipitation, western blot, three hybrid

(Kim et al., 2010)

bam - Rcd-1

pull down, molecular weight estimation by staining, colocalization, fluorescence microscopy, inferred by author, anti tag coimmunoprecipitation, anti tag western blot

(Salgania et al., 2024, Sgromo et al., 2018)

bam - Rga

anti tag coimmunoprecipitation, anti tag western blot

(Sgromo et al., 2018)

bam - Sxl

molecular sieving, western blot

(Li et al., 2013)

bam - Traf6

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Ji et al., 2019)

bam - tut

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Shan et al., 2017, Chen et al., 2014)

bam - twin

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Sgromo et al., 2018, Shan et al., 2017, Fu et al., 2015)

bam - Ubi-p5E

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot

(Ji et al., 2017)

bam - Ubi-p63E

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry

(Ji et al., 2017)

mir-ban

RNA-protein

Interacting group

Assay

References

mir-ban - AGO1

anti bait coimmunoprecipitation, northern blot, anti tag coimmunoprecipitation, nuclease footprinting, autoradiography, filter binding, competition binding, pull down, western blot

(Kakumani et al., 2020, Wen et al., 2015, Yang et al., 2014, Carré et al., 2013, Hur et al., 2013, Nishida et al., 2013, Eulalio et al., 2009, Flynt et al., 2009, Miyoshi et al., 2009, Eulalio et al., 2008, Zhou et al., 2008, Förstemann et al., 2007, Rehwinkel et al., 2006, Miyoshi et al., 2005, Okamura et al., 2004)

mir-ban - AGO2

anti tag coimmunoprecipitation, northern blot, filter binding, autoradiography

(Yang et al., 2014, Hur et al., 2013, Rehwinkel et al., 2006, Okamura et al., 2004)

mir-ban - bel

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - Dcr-1

electrophoretic mobility shift assay, autoradiography, enzymatic study, anti tag coimmunoprecipitation, northern blot

(Liu et al., 2007, Ye et al., 2007, Saito et al., 2005)

mir-ban - drosha

enzymatic study, northern blot

(Denli et al., 2004)

mir-ban - Fmr1

anti bait coimmunoprecipitation, quantitative reverse transcription pcr, northern blot, anti tag coimmunoprecipitation

(Yang et al., 2009, Okamura et al., 2004)

mir-ban - gw

anti tag coimmunoprecipitation, northern blot, anti bait coimmunoprecipitation

(Eulalio et al., 2009, Eulalio et al., 2009, Miyoshi et al., 2009, Zekri et al., 2009)

mir-ban - His1

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Liu et al., 2016)

mir-ban - loqs

anti tag coimmunoprecipitation, northern blot, anti bait coimmunoprecipitation, fluorescence polarization spectroscopy, predetermined participant

(Tants et al., 2017, Miyoshi et al., 2009, Saito et al., 2005)

mir-ban - pasha

enzymatic study, northern blot

(Denli et al., 2004)

mir-ban - Ran

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - ref(2)P

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Ghosh et al., 2024)

mir-ban - RpL21

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - RpL22

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - RpL32

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - RpS7

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - SmD1

anti bait coimmunoprecipitation, primer specific pcr

(Xiong et al., 2015)

mir-ban - Unr

pull down, western blot

(Kakumani et al., 2020)

mir-ban - vig

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

RNA-RNA

Interacting group

Assay

References

mir-ban - cic

luminiscence technology, necessary binding region

(Herranz et al., 2012)

mir-ban - Clk

luminiscence technology, necessary binding region

(Kadener et al., 2009)

mir-ban - hid

fluorescence technology, immunohistochemistry, luminiscence technology, necessary binding region

(Shen et al., 2015, Kertesz et al., 2007, Leaman et al., 2005, Robins et al., 2005, Brennecke et al., 2003)

mir-ban - Mad

luminiscence technology, necessary binding region

(Robins et al., 2005)

mir-ban - mei-P26

luminiscence technology, necessary binding region, immunohistochemistry

(Herranz et al., 2010)

mir-ban - numb

luminiscence technology, necessary binding region

(Wu et al., 2017)

mir-ban - Tgi

luminiscence technology, necessary binding region, immunohistochemistry, western blot

(Shen et al., 2015)

mir-ban - trbl

fluorescence technology

(Gerlach et al., 2019)

Alleles Reported to Model Human Disease (Disease Ontology) (22 alleles)

hop

Models Based on Experimental Evidence ( 9 )

Allele

Disease

Evidence

References

hop¹

model of hematologic cancer

CEA with hop^Tum

(Hanratty and Dearolf, 1993)

hop²

model of hematologic cancer

CEA with hop^Tum

(Hanratty and Dearolf, 1993)

hop³

model of hematologic cancer

CEA with hop^Tum

(Hanratty and Dearolf, 1993)

hop^UAS.cHa

model of disease of cellular proliferation

CEA

(Harrison et al., 1995)

hop^T42

model of hematologic cancer

CEA

(Bausek and Zeidler, 2014)

hop^Tum

model of hematologic cancer

CEA

(Yadav et al., 2021, Bailetti et al., 2019, Tettweiler et al., 2019, Bausek and Zeidler, 2014, Bina et al., 2010)

CEC

(Hanratty and Dearolf, 1993)

CEA with hop¹

(Hanratty and Dearolf, 1993)

CEA with hop²

(Hanratty and Dearolf, 1993)

CEA with hop³

(Hanratty and Dearolf, 1993)

model of disease of cellular proliferation

CEA

(Harrison et al., 1995, Luo et al., 1995)

hop^Tum.UAS

model of disease of cellular proliferation

CEA

(Harrison et al., 1995)

model of cancer

CEA with yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Ding et al., 2021)

hop^{UAS.ORF.Tag:HA}

model of hematologic cancer

CEA

(Terriente-Félix et al., 2017)

hop^GG01333

model of cancer

CEA

(Brás et al., 2021)

Modifiers Based on Experimental Evidence ( 8 )

Allele

Disease

Interaction

References

hop^HMS00761

ameliorates cancer

modeled by yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Ding et al., 2021)

hop^JF01267

exacerbates cancer

modeled by Ras85D^{G12V.UAS.Tag:FLAG,Tag:polyHis}

(Washington et al., 2020)

hop^JF01268

ameliorates cancer

modeled by Bub3^GD9924

(Barrio et al., 2023)

hop²

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Colodner and Feany, 2010)

hop^T42

model of hematologic cancer

is ameliorated by Gαf^c05874

(Bausek and Zeidler, 2014)

is ameliorated by Gαf^4B7

(Bausek and Zeidler, 2014)

is ameliorated by Stat92E^F

(Bausek and Zeidler, 2014)

is ameliorated by Stat92E³⁹⁷

(Bausek and Zeidler, 2014)

is ameliorated by Rho1^1B

(Bausek and Zeidler, 2014)

hop^Tum

model of hematologic cancer

is ameliorated by baz^UAS.cKa

(Bina et al., 2010)

is ameliorated by CG13559^GD2179

(Bina et al., 2010)

is exacerbated by mfas^GD5174

(Bina et al., 2010)

is ameliorated by baz^GD1384

(Bina et al., 2010)

is ameliorated by Stat92E^GD4492

(Bausek and Zeidler, 2014, Bina et al., 2010)

is exacerbated by CG10764^GD5293

(Bina et al., 2010)

is ameliorated by CG15211^GD13281

(Bina et al., 2010)

is ameliorated by CG4793^GD5233

(Bina et al., 2010)

is ameliorated by CG1572^GD13286

(Bina et al., 2010)

is ameliorated by Gαf^GD7540

(Bina et al., 2010)

is exacerbated by pxb^GD8472

(Bina et al., 2010)

is ameliorated by Gαf^c05874

(Bausek and Zeidler, 2014)

is exacerbated by Gαf^UAS.cBa

(Bausek and Zeidler, 2014)

is exacerbated by ush^VDRC.cUa

(Bausek and Zeidler, 2014)

is exacerbated by ush^RNAi.UAS.cUa

(Bausek and Zeidler, 2014)

is ameliorated by Hipk⁴

(Tettweiler et al., 2019)

is exacerbated by E(Pc)¹

(Bailetti et al., 2019)

is exacerbated by E(Pc)^GD12282

(Bailetti et al., 2019)

is exacerbated by E(Pc)^JF03101

(Bailetti et al., 2019)

is ameliorated by Stat92E³⁹⁷

(Bailetti et al., 2019)

is ameliorated by Tip60^E431Q.UAS

(Bailetti et al., 2019)

is exacerbated by Tip60^HM05049

(Bailetti et al., 2019)

is ameliorated by Tip60^UAS.cLa

(Bailetti et al., 2019)

is ameliorated by dome^ΔCYT.UAS

(Bailetti et al., 2019)

is ameliorated by upd3^Δ

(Bailetti et al., 2019)

hop^KK105136

DOES NOT ameliorate cancer

modeled by Hipk^UAS.Tag:HA

(Blaquiere et al., 2018)

hop^{UAS.ORF.Tag:HA}

model of hematologic cancer

is ameliorated by Stat92E^KK100519

(Terriente-Félix et al., 2017)

is ameliorated by dome^ΔCYT.UAS

(Terriente-Félix et al., 2017)

is ameliorated by lic^d13

(Terriente-Félix et al., 2017)

is ameliorated by upd3^GD6811

(Terriente-Félix et al., 2017)

bam

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

bam^Δ86

model of germ cell cancer

CEA

(Zhao et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

bam^Δ86

model of germ cell cancer

is ameliorated by Atg17^3F5

(Zhao et al., 2018)

is ameliorated by Atg6¹

(Zhao et al., 2018)

is ameliorated by Cdk2³

(Zhao et al., 2018)

is ameliorated by InR³³⁹

(Zhao et al., 2018)

mir-ban

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

mir-ban^C.UAS.EGFP

model of carcinoma

CEA with Egfr^UAS.cBa

(Herranz et al., 2012)

model of cancer

CEA with Rab5^LL00467

(Cong et al., 2021)

mir-ban^UAS.cUa

model of cancer

CEA with Egfr^UAS.cBa

(Eichenlaub et al., 2016)

Modifiers Based on Experimental Evidence ( 7 )

Allele

Disease

Interaction

References

mir-ban^{sponge.UAS.DsRed(Unk)}

ameliorates carcinoma

modeled by Ras85D^V12.UAS, l(2)gl⁴

(Ito and Igaki, 2021)

mir-ban^D.UAS.EGFP

ameliorates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

mir-ban^B90.1

ameliorates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

mir-ban^EP3208

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

mir-ban^C.UAS.EGFP

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Dubey and Tapadia, 2018)

mir-ban^Δ.as.B.UAS

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

mir-ban^EP3622

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

Alleles Representing Disease-Implicated Variants

Genetic Tools, Stocks and Reagents

Sources of Stocks

Contact lab of origin for a reagent not available from a public stock center.

Bloomington Stock Center Disease Page

Related mammalian, viral, bacterial, or synthetic transgenes

Allele

Transgene

Publicly Available Stocks

Selected Drosophila transgenes

Allele

Transgene

Publicly Available Stocks

y¹ w^*; P{UAS-hop.H}2/CyO

hop^{UAS.ORF.Tag:HA}

M{UAS-hop.ORF.3xHA}

M{UAS-hop.ORF.3xHA}ZH-86Fb

mir-ban^UAS.cUa

P{UAS-mir-ban.U}

mir-ban^D.UAS.EGFP

P{UAS-EGFP-mir-ban.D}

mir-ban^C.UAS.EGFP

P{UAS-EGFP-mir-ban.C}

w^*; P{UAS-EGFP-mir-ban.C}3

mir-ban^Δ.as.B.UAS

P{UAS-mir-ban.Delta.as.B}

mir-ban^{sponge.UAS.DsRed(Unk)}

P{UAS-dsRed.mir-ban.sponge}

RNAi constructs available

Allele

Transgene

Publicly Available Stocks

w¹¹¹⁸; P{GD9157}v40037/CyO

y¹ v¹; P{TRiP.JF01267}attP2

hop^JF01268

P{TRiP.JF01268}

y¹ v¹; P{TRiP.JF01268}attP2

hop^HMS00761

P{TRiP.HMS00761}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00761}attP2

y¹ sc^* v¹ sev²¹; P{TRiP.GL00305}attP2

w¹¹¹⁸; P{GD7155}v27347

y¹ v¹; P{TRiP.JF01435}attP2

Madm^GL01135

P{TRiP.GL01135}

y¹ sc^* v¹ sev²¹; P{TRiP.GL01135}attP2/TM3, Sb¹

Madm^HMJ02096

P{TRiP.HMJ02096}

y¹ v¹; P{TRiP.HMJ02096}attP40/CyO

Madm^HMC04156

P{TRiP.HMC04156}

y¹ v¹; P{TRiP.HMC04156}attP2/TM3, Sb¹

y¹ v¹; P{TRiP.HMS00029}attP2

y¹ v¹; P{TRiP.HMJ22155}attP40/CyO

Selected Drosophila classical alleles

Allele

Allele class

Mutagen

Publicly Available Stocks

hop^air

loss of function allele

PM hybrid dysgenesis

hop¹⁶

amorphic allele - genetic evidence

ethyl methanesulfonate

hop²

loss of function allele

X ray

hop²/FM7a

hop³⁵

amorphic allele - genetic evidence

ethyl methanesulfonate

hop³⁴

amorphic allele - genetic evidence

ethyl methanesulfonate

hop⁹

amorphic allele - genetic evidence

ethyl methanesulfonate

hop^r1

loss of function allele

gamma ray

hop¹⁴

amorphic allele - genetic evidence

ethyl methanesulfonate

hop^T42

ethyl methanesulfonate

hop¹²

amorphic allele - genetic evidence

ethyl methanesulfonate

hop^r4

loss of function allele

gamma ray

hop³³

amorphic allele - genetic evidence

ethyl nitrosourea

hop^r3

loss of function allele

gamma ray

hop³

amorphic allele - genetic evidence

X ray

y¹ w^* hop³/FM7c

hop^Tum

ethyl methanesulfonate

y¹ v¹ hop^Tum/FM7c

hop³²

amorphic allele - genetic evidence

ethyl nitrosourea

hop²⁹

amorphic allele - genetic evidence

ethyl nitrosourea

hop¹

amorphic allele - genetic evidence

ethyl methanesulfonate

hop¹³

amorphic allele - genetic evidence

ethyl methanesulfonate

hop^r2

loss of function allele

gamma ray

hop⁴

amorphic allele - genetic evidence

X ray

hop^r5

loss of function allele

gamma ray

hop¹⁵

amorphic allele - genetic evidence

ethyl methanesulfonate

hop²⁷

amorphic allele - genetic evidence

ethyl nitrosourea

y¹ hop²⁷/FM7c

hop^GG01333

y¹ w^67c23 P{Mae-UAS.6.11}hop^GG01333

bam^{null-In2-3xP3-DsRed}

loss of function allele

CRISPR/Cas9

bam^Δ86

loss of function allele

P-element activity

ry⁵⁰⁶ e¹ bam^Δ86/TM3, ry^RK Sb¹ Ser¹

P-element activity

P-element activity

Delta2-3 transposase

amorphic allele - genetic evidence

Delta2-3 transposase

References (17)

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