FlyBase Gene Report: Dmel\ena

General Information

Symbol

Dmel\ena

Species

D. melanogaster

Name

enabled

Annotation Symbol

CG15112

Feature Type

protein_coding_gene

FlyBase ID

FBgn0000578

Gene Model Status

Current

Stock Availability

25 publicly available

Gene Summary

enabled (ena) encodes the sole Drosophila member of the Ena/VASP family. It acts as a processive actin polymerase, stimulating actin addition at the barbed end. It has roles in both epithelial morphogenesis and CNS pathfinding. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

VASP, l(2)02029, enb

Key Links

Genomic Location

Cytogenetic map

56B5-56C1

Sequence location

2R:19,141,632..19,163,705 [+]

Recombination map

2-88

RefSeq locus

NT_033778 REGION:19141632..19163705

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (37 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:Zyx; FB:FBgn0011642

(Renfranz et al., 2003)

inferred from physical interaction with UniProtKB:O44924

(Bashaw et al., 2000)

inferred from physical interaction with UniProtKB:P25843

(Ahern-Djamali et al., 1999)

inferred from physical interaction with FLYBASE:Abi; FB:FBgn0020510

(Chen et al., 2014)

enables SH3 domain binding

inferred from physical interaction with UniProtKB:P00522

(Gertler et al., 1995)

inferred from direct assay

(Ahern-Djamali et al., 1999)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (25 terms)

Terms Based on Experimental Evidence (24 terms)

CV Term

Evidence

References

involved_in actin cytoskeleton organization

inferred from mutant phenotype

(Lin et al., 2009)

involved_in actin filament organization

inferred from mutant phenotype

(Harker et al., 2019, Gates et al., 2009, Lyulcheva et al., 2008)

involved_in axon guidance

inferred from genetic interaction with FLYBASE:robo1; FB:FBgn0005631

(Bashaw et al., 2000)

inferred from mutant phenotype

(Wills et al., 1999)

involved_in axonogenesis

inferred from mutant phenotype

(Bashaw et al., 2000)

involved_in border follicle cell migration

inferred from mutant phenotype

(Lucas et al., 2013, Gates et al., 2009)

involved_in compound eye development

inferred from genetic interaction with FLYBASE:cindr; FB:FBgn0027598

(Johnson and Cagan, 2009)

involved_in dendrite morphogenesis

inferred from mutant phenotype

(Dimitrova et al., 2008, Li et al., 2005, Gao et al., 1999)

involved_in dorsal closure

inferred from genetic interaction with FLYBASE:arm; FB:FBgn0000117

(Grevengoed et al., 2001)

involved_in epithelial cell morphogenesis

inferred from genetic interaction with FLYBASE:Abl; FB:FBgn0000017

(Grevengoed et al., 2001)

involved_in filopodium assembly

inferred from mutant phenotype

(Segal et al., 2016)

involved_in formation of a compartment boundary

inferred from mutant phenotype

(Becam et al., 2011)

involved_in germ-band shortening

inferred from mutant phenotype

(Gates et al., 2007)

involved_in gonad morphogenesis

inferred from mutant phenotype

(Sano et al., 2012)

involved_in head involution

inferred from mutant phenotype

(Gates et al., 2007)

involved_in negative regulation of synaptic assembly at neuromuscular junction

inferred from mutant phenotype

(Loya et al., 2014, Loya et al., 2009)

involved_in ovarian nurse cell to oocyte transport

inferred from mutant phenotype

(Gates et al., 2009)

involved_in positive regulation of cell migration

inferred from mutant phenotype

(Tucker et al., 2011)

involved_in positive regulation of cell projection organization

inferred from direct assay

(Tucker et al., 2011)

inferred from mutant phenotype

(Tucker et al., 2011)

involved_in positive regulation of filopodium assembly

inferred from mutant phenotype

(Harker et al., 2019, Gonçalves-Pimentel et al., 2011, Gates et al., 2007)

involved_in regulation of actin cytoskeleton organization

inferred from physical interaction with FLYBASE:Abi; FB:FBgn0020510

(Chen et al., 2014)

involved_in regulation of filopodium assembly

inferred from mutant phenotype

(Homem and Peifer, 2009)

involved_in regulation of lamellipodium assembly

inferred from mutant phenotype

(Homem and Peifer, 2009)

involved_in subsynaptic reticulum organization

inferred from mutant phenotype

(Loya et al., 2014)

involved_in suture of dorsal opening

inferred from mutant phenotype

(Gates et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in actin polymerization or depolymerization

inferred from biological aspect of ancestor with PANTHER:PTN000128518

(GO Reference Genome Project, 2011-)

involved_in axon guidance

inferred from biological aspect of ancestor with PANTHER:PTN000128518

(GO Reference Genome Project, 2011-)

Cellular Component (10 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

colocalizes_with adherens junction

inferred from direct assay

(Gates et al., 2007, Grevengoed et al., 2001)

located_in axon

inferred from direct assay

(Li et al., 2005, Grevengoed et al., 2001)

located_in cell leading edge

inferred from direct assay

(Gates et al., 2007)

colocalizes_with cell-cell junction

inferred from direct assay

(Renfranz et al., 2003)

located_in dendrite

inferred from direct assay

(Li et al., 2005)

located_in filopodium tip

inferred from direct assay

(Tucker et al., 2011, Gates et al., 2007)

located_in lamellipodium

inferred from direct assay

(Biyasheva et al., 2004)

inferred from direct assay

(Tucker et al., 2011)

located_in subsynaptic reticulum

inferred from direct assay

(Loya et al., 2014)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in filopodium tip

inferred from biological aspect of ancestor with PANTHER:PTN002598160

(GO Reference Genome Project, 2011-)

is_active_in focal adhesion

inferred from biological aspect of ancestor with PANTHER:PTN000128518

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN002598102

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the Ena/VASP family. (Q8T4F7)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Protein Function (UniProtKB)

Functions, together with Abl, trio and fra, in a complex signaling network that regulates axon guidance at the CNS midline. Required in part for robo-mediated repulsive axon guidance. May be involved in lamellipodial dynamics.

(UniProt, Q8T4F7)

Summary (Interactive Fly)

VASP homolog and SH3 domain protein - acts as processive actin polymerase, stimulating actin addition at the barbed end - cytoskeletal adaptor protein acting in epithelial morphogenesis - implicated in axonal outgrowth and fasciculation

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ena for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8T4F7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ena-RA

FBtr0086583

NM_166329

3370

684

ena-RB

FBtr0086584

NM_166331

3669

834

ena-RC

FBtr0086582

NM_166330

3806

684

ena-RD

FBtr0100174

NM_001014537

2878

687

ena-RE

FBtr0100176

NM_001014536

3446

684

ena-RF

FBtr0114623

NM_001144237

4107

980

ena-RG

FBtr0340450

NM_001299673

4417

664

Additional Transcript Data and Comments

Reported size (kB)

3.4 (longest cDNA)

(Gertler et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ena-PA

FBpp0085767

72.0

684

9.65

NP_725857

AAF57598

ena-PB

FBpp0085768

89.3

834

9.59

NP_725859

AAM68439

ena-PC

FBpp0085766

72.0

684

9.65

NP_725858

AAM68438

ena-PD

FBpp0099530

72.3

687

9.73

NP_001014537

AAX52696

ena-PE

FBpp0099532

72.0

684

9.65

NP_001014536

AAX52697

ena-PF

FBpp0113115

104.8

980

9.89

FBpp0309392

69.6

664

9.80

A0A0B4LFX0

NP_001286602

AHN56397

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

684 aa isoforms: ena-PA, ena-PC, ena-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

684 (aa); 80 (kD observed); 72 (kD predicted)

(Gertler et al., 1995)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Abl and Src SH3 domains. Binds, in vitro and in vivo, the cytoplasmic domain of robo. Interacts with Zyx102EF and chic.

(UniProt, Q8T4F7)

Post Translational Modification

Tyrosine phosphorylated on multiple sites by Abl kinase. In vitro, phosphorylation on specific tyrosine residues inhibits interaction with Abl and Src SH3 domains.

(UniProt, Q8T4F7)

Domain

The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

(UniProt, Q8T4F7)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ena using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ena_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.74

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

segment | segment boundary

(Grevengoed et al., 2001)

presumptive embryonic/larval central nervous system | restricted

(Grevengoed et al., 2001)

neuron

• axon

(Grevengoed et al., 2001)

embryonic leading edge cell

• adherens junction

(Grevengoed et al., 2001)

embryonic hindgut

• adherens junction

(Grevengoed et al., 2001)

leading edge mixer cell | subset

(Gettings et al., 2010)

embryonic stage 5 -- 15

organism | ubiquitous

• adherens junction & cytoplasm

(Gates et al., 2007)

amnioserosa

• cell cortex

(Gates et al., 2007)

embryonic stage 8 -- 11

• adherens junction

(Grevengoed et al., 2001)

embryonic stage 10 -- 11

endoderm

(Gertler et al., 1995)

embryonic stage 12 -- 13

fat body/gonad primordium

(Sano et al., 2012)

embryonic stage 12 -- 14

presumptive embryonic/larval central nervous system | presumptive

(Gertler et al., 1995)

dorsal closure stage

metameric furrow

(Gates et al., 2007)

embryonic stage 13 -- 15

embryonic leading edge cell

(Gates et al., 2007)

amnioserosa

(Gates et al., 2007)

embryonic stage 14 -- 15

gonadal sheath proper primordium

(Sano et al., 2012)

embryonic stage 15 -- 16

larval ventral nerve cord commissure

(Gertler et al., 1995)

embryonic stage 15 -- 17

larval longitudinal connective

(Gertler et al., 1995)

larval stage

adepithelial cell

• adherens junction

(Grevengoed et al., 2001)

imaginal disc | restricted

• adherens junction

(Grevengoed et al., 2001)

rhabdomere

• adherens junction

(Grevengoed et al., 2001)

photoreceptor neuron

• adherens junction

(Grevengoed et al., 2001)

spermatogenesis & adult stage | male

male germline stem cell

• plasma membrane | restricted

(Srinivasan et al., 2012)

oogenesis

follicle cell

(Grevengoed et al., 2001)

oogenesis stage S11 -- S12

follicle cell

(Wahlstrom et al., 2006)

Additional Descriptive Data

ena protein is expressed in somatic gonadal precursor cells from embryonic stage 12 though gonad coalescence.

(Sano et al., 2012)

ena localizes to the surface of male germline stem cells facing the hub.

(Srinivasan et al., 2012)

ena protein localizes to adherens junctions and the cytoplasm, from after cellularization, through gastrulation and into the extended germband stage. After germband extension, ena protein remains at adherens junctions and concentrates at epidermal tricellular junctions; it is uniformly localized around the amnioserosal cell cortex. During dorsal closure, ena protein becomes concentrated along the front of leading-edge cells, becoming strongly enriched in spots at leading edge adherens junctions, but remaining at tricellular junctions. ena protein is especially enriched in the single row of cells per segment that initiate segmental grooves and in cells at the anterior edge of the dorsal fold that lead during head involution.

(Gates et al., 2007)

ena protein localizesto adherens junctions.

(Wahlstrom et al., 2006, Grevengoed et al., 2001)

During the initialstages of dorsal closure, as leading edge cells begin to elongate, ena protein surrounds the cell membrane but is enriched at sites of cell-cell contact and at adherens junctions of leading edge cells. As closure proceeds, ena protein accumulates at uniformly high levels at the adherens junctions of leading edge cells. ena protein localizes to the cytoplasm in a stripe of epidermal cells at the segmental boundary. ena protein is also observed in the embryonic hindgut, in axons of the embryonic CNS, in adherens junctions of imaginal disc epithelial cells, and in the specialized junctions of the photoreceptor rhabdomeres.

(Grevengoed et al., 2001)

ena protein is first detected in embryonic stage 10 and by stage 11 is found in the endoderm and ectoderm of the extended germ band. During germ band retraction, expression becomes restricted to the CNS and by stage 15 is found primarily in the commissural and longitudinal axons of the CNS. During late embryogenesis, ena is localized primarily to the longitudinal axons.

(Gertler et al., 1995)

Marker for

Subcellular Localization

CV Term

Evidence

References

colocalizes_with adherens junction

inferred from direct assay

(Gates et al., 2007, Grevengoed et al., 2001)

located_in axon

inferred from direct assay

(Li et al., 2005, Grevengoed et al., 2001)

located_in cell leading edge

inferred from direct assay

(Gates et al., 2007)

colocalizes_with cell-cell junction

inferred from direct assay

(Renfranz et al., 2003)

located_in dendrite

inferred from direct assay

(Li et al., 2005)

located_in filopodium tip

inferred from direct assay

(Tucker et al., 2011, Gates et al., 2007)

located_in lamellipodium

inferred from direct assay

(Biyasheva et al., 2004)

inferred from direct assay

(Tucker et al., 2011)

located_in subsynaptic reticulum

inferred from direct assay

(Loya et al., 2014)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ena in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ena

View exonic expression by tissue for Dmel\ena

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000578

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37201/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000578

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000578

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000578

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15112

Flygut - An atlas of the Drosophila adult midgut

FBgn0000578

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ena

Stages(s) 1-3
Stages(s) 7-8
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 47 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 23 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ena

Transgenic constructs containing regulatory region of ena

Aberrations (Deficiencies and Duplications) ( 15 )

Inferred from experimentation ( 15 )

Gene not disrupted in

Df(2R)F7

(Bradley and Andrew, 2001)

Df(2R)PC29

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

lethal - all die during embryonic stage (with ena²³)

ena^GC1

lethal - all die during embryonic stage (with ena^GC1)

lethal - all die during embryonic stage (with ena^GC5)

ena^GC1

lethal - all die during embryonic stage | recessive

partially lethal - majority die

ena⁰²⁰²⁹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ena^GD8910

viable, with Scer\GAL4^Mef2.PR

ena^GD8910

viable, with Scer\GAL4^pnr-MD237

ena^GD8910

viable, with Scer\GAL4^tin.CΔ4

ena^GD8910

Other Phenotypes

Allele

abnormal cell migration, with Scer\GAL4^slbo.2.6

abnormal cell shape

ena²³

abnormal cell shape | embryonic stage

ena^GC5

abnormal cell shape | P-stage, with Scer\GAL4^kirre.PU

ena^GD8910

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

ena^UAS.cADa

abnormal neuroanatomy, with Scer\GAL4^how-24B

ena^{UAS.Tag:polyHis}

abnormal neuroanatomy (with ena^GC1)

ena^GC5

abnormal neuroanatomy (with ena^GC5)

abnormal neuroanatomy (with ena^GC8)

ena^GC5

abnormal neuroanatomy | adult stage | somatic clone

abnormal neuroanatomy | embryonic stage

ena⁴⁶

abnormal neuroanatomy | embryonic stage (with ena²³)

ena^GC1

abnormal neuroanatomy | embryonic stage (with ena²¹⁰)

ena^GC1

abnormal neuroanatomy | embryonic stage (with ena^GC1)

abnormal neuroanatomy | embryonic stage (with ena^GC5)

ena^GC1

abnormal neuroanatomy | larval stage | somatic clone

ena²¹⁰

abnormal neuroanatomy | somatic clone

abnormal neuroanatomy | somatic clone | third instar larval stage

abnormal neuroanatomy | third instar larval stage

ena⁴⁶

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^109(2)80

ena^UAS.cUa

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^how-24B

ena^{UAS.Tag:polyHis}

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

ena^HMS01953

abnormal size, with Scer\GAL4^Bx-MS1096

ena^{UAS.Tag:polyHis}

decreased rate of cell adhesion | embryonic stage

ena^GC5

increased cell number | embryonic stage, with Scer\GAL4^E4

ena^UAS.cUa

majority die during embryonic stage

ena²³

some die during embryonic stage (with ena²³)

ena^GC1

some die during embryonic stage (with ena^GC1)

some die during embryonic stage (with ena^GC5)

ena^GC1

some die during embryonic stage | recessive

visible, with Scer\GAL4^pnr-MD237

ena^GD8910

visible | adult stage, with Scer\GAL4^nub.PU

ena^UAS.cGb

wild-type

Phenotype manifest in

Allele

abdominal posterior ventral multidendritic neuron vdap

ena⁴⁶

abdominal posterior ventral multidendritic neuron vdap, with Scer\GAL4^109(2)80

ena^UAS.cUa

abdominal posterior ventral multidendritic neuron vdap | somatic clone

ena^GC1

actin filament & egg chamber | germ-line clone

adult mushroom body, with Scer\GAL4^ey-OK107

ena^UAS.cADa

adult mushroom body | somatic clone

adult mushroom body gamma-lobe | somatic clone

axon

axon | adult stage | somatic clone

border follicle cell, with Scer\GAL4^slbo.2.6

central nervous system (with ena²¹⁰)

ena^GC1

central nervous system (with ena^GC1)

ena²¹⁰

dendrite

ena⁴⁶

dendrite, with Scer\GAL4^109(2)80

ena^UAS.cUa

dendrite | dorsal | embryonic stage

ena⁴⁶

dendrite | larval stage

ena²¹⁰

dendrite | somatic clone

dendritic arborizing neuron

ena⁴⁶

dendritic branch | decreased number | larval stage | somatic clone

ena²¹⁰

dendritic tree

ena⁴⁶

dorsal-ventral compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^sd-SG29.1

egg | germline clone | maternal effect

egg | maternal effect, with Scer\GAL4^GR1

ena^HMS01953

egg chamber, with Scer\GAL4^E4

ena^UAS.cUa

egg chamber | clone of cells, with Scer\GAL4^Tub.PU

ena^UAS.cUa

embryo | dorsal closure stage

ena²³

embryo | embryonic stage 14 (with ena²¹⁰)

ena^GC1

embryo | embryonic stage 14 (with ena^GC1)

ena²¹⁰

embryonic/first instar larval cuticle

embryonic/first instar larval cuticle (with ena^GC1)

ena^GC5

embryonic/first instar larval cuticle (with ena^GC5)

ena^GC1

embryonic/first instar larval cuticle | maternal effect

embryonic/larval aorta | embryonic stage

ena^GC5

embryonic/larval heart | embryonic stage

embryonic/larval neuromuscular junction, with Scer\GAL4^how-24B

ena^{UAS.Tag:polyHis}

embryonic cardioblast

embryonic dorsal epidermis

embryonic dorsal epidermis (with ena^GC1)

ena^GC5

embryonic dorsal epidermis (with ena^GC5)

ena^GC1

embryonic head

ena²³

embryonic head | maternal effect

ena²³

embryonic head epidermis | maternal effect

embryonic leading edge cell

ena²³

filopodium

filopodium, with Scer\GAL4^en-e16E

ena^UAS.GFP

filopodium (with ena²³)

ena^GC1

filopodium (with ena^GC1)

ena²³

filopodium & embryonic leading edge cell, with Scer\GAL4^en-e16E

ena^UAS.GFP

filopodium | cell autonomous | clone of cells | ectopic | oogenesis, with Scer\GAL4^Tub.PU

ena^UAS.cUa

filopodium | ectopic, with Scer\GAL4^en-e16E

ena^UAS.GFP

filopodium | P-stage, with Scer\GAL4^kirre.PU

ena^GD8910

follicle cell & actin filament, with Scer\GAL4^CY2

ena^{UAS.Tag:polyHis}

follicle cell & actin filament | somatic clone

ena²³

follicle cell | cell autonomous | clone of cells | ectopic | oogenesis, with Scer\GAL4^Tub.PU

ena^UAS.cUa

germline cell | embryonic stage

ena^C14-06

gonad | embryonic stage

ena^C14-06

gonadal sheath proper primordium

ena^C14-06

indirect flight muscle cell | P-stage, with Scer\GAL4^kirre.PU

ena^KK107752

lamellipodium

ena²³

larval dorsal multidendritic neuron ddaB | somatic clone

ena⁴⁶

larval dorsal multidendritic neuron ddaC | somatic clone

ena⁴⁶

larval dorsal multidendritic neuron ddaE | somatic clone

larval dorsal multidendritic neuron ddaF | somatic clone

ena⁴⁶

larval intersegmental nerve

larval intersegmental nerve branch ISNb of A1-7 (with ena^GC1)

ena^GC5

larval intersegmental nerve branch ISNb of A1-7 (with ena^GC5)

ena^GC1

larval longitudinal connective

larval longitudinal connective (with ena²³)

ena^GC1

larval longitudinal connective (with ena²¹⁰)

ena^GC1

larval longitudinal connective (with ena^GC1)

larval longitudinal connective (with ena^GC5)

ena^GC1

larval multidendritic class III neuron | somatic clone

ena²¹⁰

larval multidendritic class IV neuron

ena^unspecified

macrochaeta

ena^BG02189

myotube | P-stage, with Scer\GAL4^kirre.PU

NMJ bouton, with Scer\GAL4^how-24B

ena^{UAS.Tag:polyHis}

NMJ bouton | increased number, with Scer\GAL4^RapGAP1-OK6

ena^HMS01953

NMJ bouton | third instar larval stage, with Scer\GAL4^how-24B

ena^{UAS.Tag:polyHis}

nurse cell & actin filament, with Scer\GAL4^{VP16.mat.αTub67C}

ena^{UAS.Tag:polyHis}

oocyte, with Scer\GAL4^E4

ena^UAS.cUa

peripheral nervous system

perivitelline space, with Scer\GAL4^E4

ena^UAS.cUa

presumptive embryonic/larval central nervous system (with ena^GC1)

ena^GC5

presumptive embryonic/larval central nervous system (with ena^GC5)

presumptive embryonic/larval central nervous system (with ena^GC8)

ena^GC5

primordial germ cell | increased number, with Scer\GAL4^E4

ena^UAS.cUa

RP neuron

subsynaptic reticulum | third instar larval stage, with Scer\GAL4^how-24B

ena^{UAS.Tag:polyHis}

symmetrical commissure

symmetrical commissure (with ena²¹⁰)

ena^GC1

symmetrical commissure (with ena^GC1)

ena²¹⁰

trichogen cell, with Scer\GAL4^pnr-MD237

ena^GD8910

ventral head epidermis

ena²³

wing, with Scer\GAL4^Bx-MS1096

ena^{UAS.Tag:polyHis}

wing blade, with Scer\GAL4^nub.PU

ena^UAS.cGb

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (9)

Hsap\ENAH

10 of 14

Yes

Hsap\EVL

7 of 14

Yes

Hsap\VASP

6 of 14

Yes

3 of 14

3 of 14

3 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

Rnor\Enah

10 of 14

Yes

Rnor\Evl

7 of 14

Yes

Rnor\Vasp

5 of 14

Yes

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (9)

Mmus\Enah

11 of 14

Yes

Mmus\Evl

7 of 14

Yes

Mmus\Vasp

5 of 14

Yes

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (13)

Xtro\enah

6 of 13

Yes

Xtro\evl

3 of 13

Yes

Xtro\vasp

2 of 13

Yes

Xtro\fkbpl

1 of 13

Yes

Xtro\gkap1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (13)

Drer\enah

10 of 14

Yes

Drer\vaspb

6 of 14

Yes

Drer\evla

5 of 14

Yes

Drer\evlb

5 of 14

Yes

Drer\vaspa

4 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\unc-34

7 of 14

Yes

Anopheles gambiae (African malaria mosquito) (3)

Agam\AgaP_AGAP010673

9 of 12

Yes

Agam\AgaP_AGAP012085

2 of 12

Agam\AgaP_AGAP010076

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ena. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (3)

Spred

4 of 13

CG3588

1 of 13

homer

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

cancer, multiple, actin-remodeling regulators

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 6 )

Allele

Disease

Interaction

References

ena²¹⁰

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

ena²³

ameliorates chronic myeloid leukemia

modeled by Hsap\ABL1::Hsap\BCR^UAS.cBa

(Bernardoni et al., 2019)

ena^GD8910

ameliorates chronic myeloid leukemia

modeled by Hsap\ABL1::Hsap\BCR^UAS.cBa

(Bernardoni et al., 2019)

ena^KK107752

ameliorates chronic myeloid leukemia

modeled by Hsap\ABL1::Hsap\BCR^UAS.cBa

(Bernardoni et al., 2019)

ena^HMS01953

exacerbates autism spectrum disorder

modeled by Abca3^GD3708

(Ueoka et al., 2020)

ena^{UASp.B.mCherry}

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-231.UAS}

(Onur et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ENAH; ENAH actin regulator

10 of 14

609061

EVL; Enah/Vasp-like

7 of 14

616912

VASP; vasodilator stimulated phosphoprotein

6 of 14

601703

SPRED1; sprouty related EVH1 domain containing 1

3 of 14

609291

LEGIUS SYNDROME; LGSS

Legius syndrome

SPRED2; sprouty related EVH1 domain containing 2

3 of 14

609292

NOONAN SYNDROME 14; NS14

SPRED3; sprouty related EVH1 domain containing 3

3 of 14

609293

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

ena

Hsap\VASP

(Ahern-Djamali et al., 1998)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ena

two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Ahern-Djamali et al., 1999, Ahern-Djamali et al., 1998)

ena - Abi

far western blotting, peptide array, anti tag western blot, pull down, western blot

(Chen et al., 2014)

ena - Abl

far western blotting, anti tag western blot, pull down, western blot, enzymatic study, autoradiography, peptide massfingerprinting

(Ahern-Djamali et al., 1999, Wills et al., 1999, Ahern-Djamali et al., 1998, Comer et al., 1998, Gertler et al., 1995)

ena - Atg9

two hybrid

(Kiss et al., 2020)

ena - DAAM

anti tag coimmunoprecipitation, anti tag western blot

(Matusek et al., 2008)

ena - Khc

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, two hybrid

(Martin et al., 2005)

ena - Lar

pull down, western blot, anti tag coimmunoprecipitation, enzymatic study, autoradiography

(Johnson et al., 2006, Wills et al., 1999)

ena - Ptp10D

pull down, western blot

(Wills et al., 1999)

ena - Ptp69D

pull down, western blot

(Wills et al., 1999)

ena - Rop

experimental knowledge based

(Guruharsha et al., 2011)

ena - SCAR

pull down, western blot

(Chen et al., 2014)

ena - Zyx

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, autoradiography

(Gaspar et al., 2015, Renfranz et al., 2003)

ena - chic

pull down, western blot, two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Kiss et al., 2020, Matusek et al., 2008, Ahern-Djamali et al., 1999)

ena - dia

anti tag coimmunoprecipitation, western blot, two hybrid, bimolecular fluorescence complementation, fluorescence microscopy, pull down

(Bilancia et al., 2014, Homem and Peifer, 2009)

ena - drk

pull down, western blot

(Ahern-Djamali et al., 1999)

ena - ft

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

ena - pico

anti tag coimmunoprecipitation, western blot, two hybrid

(Lyulcheva et al., 2008)

ena - robo1

pull down, autoradiography, western blot, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation

(Fan et al., 2003, Bashaw et al., 2000)

RNA-RNA

Physical Interaction

Assay

References

ena - mir-8

immunohistochemistry, fluorescence technology, necessary binding region

(Loya et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ena

suppressible

chic

(Yaniv et al., 2020)

ena

enhanceable

cno

(Choi et al., 2011)

ena

suppressible

DAAM

(Yaniv et al., 2020)

ena

enhanceable

IRSp53

(Segal et al., 2016)

ena

enhanceable

(Crowner et al., 2003)

enhanceable

(Li et al., 2005)

suppressible

(Li et al., 2005)

suppressible

(Lyulcheva et al., 2008)

ena

enhanceable

pyd

(Choi et al., 2011)

ena

enhanceable

robo1

(Bashaw et al., 2000)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Abl

suppressible

ena

(Sun et al., 2024, Grevengoed et al., 2003, Wills et al., 2002, Grevengoed et al., 2001, Liebl et al., 2000, Gertler et al., 1990)

Abl|Khc

suppressible

ena

(Martin et al., 2005)

Abl|Nrt

suppressible

ena

(Gertler et al., 1990)

Abl|fax

suppressible

ena

(Liebl et al., 2000)

Abl|trio

suppressible

ena

(Forsthoefel et al., 2005, Liebl et al., 2000)

Atg9

enhanceable

chic|ena

(Kiss et al., 2020)

Atg9

enhanceable

ena

(Kiss et al., 2020)

cindr

enhanceable

ena

(Johnson and Cagan, 2009)

cno

enhanceable

ena

(Choi et al., 2011)

comm

suppressible

ena

(Bashaw et al., 2000)

Csk

suppressible

ena

(Rudrapatna et al., 2014)

DAAM

suppressible

ena

(Matusek et al., 2008)

DAAM

enhanceable

ena

(Matusek et al., 2008)

Dab

suppressible

ena

(Song et al., 2010)

Dora

suppressible

ena

(Molina-Pelayo et al., 2022)

enhanceable

ena

(Buckles et al., 2001)

fra::robo1

suppressible

ena

(Bashaw et al., 2000)

fra

suppressible

ena

(Forsthoefel et al., 2005)

hep

suppressible

ena

(Rudrapatna et al., 2014)

hpo

suppressible

ena

(Lucas et al., 2013)

Lar

enhanceable

ena

(Maurel-Zaffran et al., 2001)

Lar

suppressible

ena

(Maurel-Zaffran et al., 2001)

mir-8

suppressible

ena

(Loya et al., 2009)

suppressible

ena

(Kuzina et al., 2011)

enhanceable

ena

(Crowner et al., 2003)

Nrt|Abl

suppressible

ena

(Liebl et al., 2000)

pico

suppressible

ena

(Lyulcheva et al., 2008)

pyd

enhanceable

ena

(Choi et al., 2011)

Rho1

suppressible

ena

(Rudrapatna et al., 2014)

robo1

suppressible

ena

(Bashaw et al., 2000)

robo1|sli

suppressible

ena

(Keleman et al., 2005)

robo2

suppressible

ena

(Bashaw et al., 2000, Simpson et al., 2000)

Shrm

suppressible

ena

(Hildebrand et al., 2021)

sli

enhanceable

robo1|ena

(Bashaw et al., 2000)

sli|robo1

enhanceable

ena

(Bashaw et al., 2000)

Strip

suppressible

ena

(Sakuma et al., 2016)

trio

suppressible

ena

(Liebl et al., 2000)

Zyx

enhanceable

ena

(Gaspar et al., 2015)

External Data

Subunit Structure (UniProtKB)

Interacts with Abl and Src SH3 domains. Binds, in vitro and in vivo, the cytoplasmic domain of robo. Interacts with Zyx102EF and chic.

(UniProt, Q8T4F7 )

Linkouts

BioGRID - A database of protein and genetic interactions.

62866

DroID - A comprehensive database of gene and protein interactions.

FBgn0000578

MIST (genetic) - An integrated Molecular Interaction Database

37201

MIST (protein-protein) - An integrated Molecular Interaction Database

37201

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Cell-extracellular matrix interactions

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-88

Cytogenetic map

56B5-56C1

Sequence location

2R:19,141,632..19,163,705 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

56B5-56C1

Limits computationally determined from genome sequence between P{lacW}prod^k08810 and P{PZ}ena⁰²⁰²⁹&P{lacW}cora^k08713

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

55F-56C

(Gao et al., 1999)

56C1-56C2

(determined by in situ hybridisation)

(Spradling et al., 1999)

56C1-56C2

(BDGP Project Members, 1994-1999)

Determined by deficiency mapping (details unspecified).

(Gao et al., 1999)

Experimentally Determined Recombination Data

Location

2-88

(FlyBase, 2016)

2-93.6

(Comeron et al., 2012)

2-87

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (25)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

28798

w^*; P{Ubi-GFP.ena}3

29959

w¹¹¹⁸; P{UAS-ena.His6}3

83021

y¹; M{WKO.1-C12}ZH-86Fb

8571

ena²³/CyO, P{act-lacZ.B}CB1

66169

w^*; P{UASp-mCherry.ena}attP2

9139

y¹ w^*; P{UAS-ena.AD}3

58744

P{UAS-mRFP.ena}1, y¹ w^*

31582

y¹ v¹; P{TRiP.JF01155}attP2

85658

w¹¹¹⁸; PBac{WH}ena^f03225/CyO

VDRC

v106484

P{KK107752}VIE-260B

v43056

w¹¹¹⁸; P{GD8910}v43056

v43058

w¹¹¹⁸; P{GD8910}v43058/CyO

More stocks available...

Genomic Clones (29)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (84)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_LD23655

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0000578

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0000578

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Bashaw et al., 2000)

polyclonal

(Gertler et al., 1995)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

5G2_anti-enabled

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: reduced F-actin and altered cell shape. Kc167 cells show change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

ena mediates part of robo's repulsive signal in the central nervous system.

(Bashaw et al., 2000)

ena protein can bind directly to chic protein. The proline-rich region of ena is responsible for this interaction.

(Ahern-Djamali et al., 1999)

ena has a function in dendritic development.

(Gao et al., 1999)

ena protein is directly phosphorylated by Abl protein.

(Comer et al., 1998)

Mutant phenotype indicates a role for the ena protein in the organisation of the embryonic PNS and CNS. A critical function of Abl is to phosphorylate and negatively regulate ena protein during neural development.

(Gertler et al., 1995)

Alleles act as suppressors of Abl mutant phenotype.

(Bennett and Hoffmann, 1992)

Mutations in ena suppress the Abl mutant phenotype.

(Gertler et al., 1990)

A screening procedure was used to recover second-site mutations that would shift the lethal phase of Abl mutant homozygotes and Nrt mutant heterozygotes from embryonic or larval stages back to pupal.

(Gertler et al., 1990)

Relationship to Other Genes

Source for database merge of

Source for merge of: enb l(2)02029

(Gao et al., 1999, Spradling et al., 1999)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG15112

(Horan et al., 2015, Hosono et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ni et al., 2008, Norga et al., 2003, Rogers et al., 2003, Labourier et al., 2002, Goldstein and Gunawardena, 2000)

ENA/VASP

(Goldstein and Gunawardena, 2000)

Ena

(Fischer et al., 2024, Fernandez-Gonzalez and Harris, 2023, Heron et al., 2023, Ecovoiu et al., 2022, Logan et al., 2022, Molina-Pelayo et al., 2022, Deng et al., 2021, Grintsevich et al., 2021, Kilo et al., 2021, Marques-Reis and Moreno, 2021, Paraskevopoulos and McGuigan, 2021, Ricolo et al., 2021, Al Outa et al., 2020, Baker, 2020, Davidson and Wood, 2020, Kanda and Igaki, 2020, Ko and Martin, 2020, La Marca and Richardson, 2020, Martin, 2020, Popkova et al., 2020, Meltzer et al., 2019, Enomoto et al., 2018, Franz et al., 2018, Richardson and Portela, 2018, Baker, 2017, Gilmour et al., 2017, Richardson and Portela, 2017, Ducuing and Vincent, 2016, Rogers et al., 2016, Flores-Benitez and Knust, 2015, Gaspar et al., 2015, Loya et al., 2014, Lu et al., 2014, Nowotarski et al., 2014, Wang et al., 2014, Winkelman et al., 2014, Huelsmann et al., 2013, Lucas et al., 2013, Matsui and Lai, 2013, Sander et al., 2013, Weavers and Skaer, 2013, Coló et al., 2012, Förster and Luschnig, 2012, Gates, 2012, Najafov et al., 2012, Nose, 2012, Wang et al., 2012, Laplante and Nilson, 2011, Razzell et al., 2011, Tucker et al., 2011, Oliva and Sierralta, 2010, Rousset et al., 2010, Sánchez-Soriano et al., 2010, Delon and Brown, 2009, Fricke et al., 2009, Loya et al., 2009, Mulinari and Häcker, 2009, Alves-Silva et al., 2008, Homem and Peifer, 2008, Nowotarski et al., 2008, Santiago-Martínez et al., 2008, Stevens et al., 2008, Vernier and Stevens, 2008, Vincent et al., 2008, Artabazon et al., 2007, Gates et al., 2007, Johnson et al., 2006, Price et al., 2006, Bogdan et al., 2005, Leyssen et al., 2005, Martin et al., 2005, Merianda et al., 2005, Biyasheva et al., 2004, Martin et al., 2004, Baas and Luo, 2001, Bateman and Van Vactor, 2001, Baum et al., 2001, Giger and Kolodkin, 2001, Grevengoed et al., 2001, Ramel and Katz, 2001, Suzuki et al., 2001, Bashaw et al., 2000, De Strooper and Annaert, 2000, Lin and Greenberg, 2000, Machesky, 2000, Takagi et al., 2000, Ui-Tei and Miyata, 2000, Andersen, 1999, Gallo and Letourneau, 1999, Garrity, 1999, Siegrist et al., 1998, Wills et al., 1998, Wills et al., 1998)

Enabled

(Perez-Vale and Peifer, 2020, West and Harris, 2020)

Enb

(Pawson and Nash, 2000)

VASP

(Gou et al., 2023, Mickael et al., 2021, Nose, 2012, Laplante and Nilson, 2011)

Vasp

(Förster and Luschnig, 2012)

ena

(Jacobs and Luschnig, 2025.5.14, Jacobs et al., 2025, Garcia-Vaquero et al., 2023, Beaver et al., 2022, Hultmark and Andó, 2022, Ramnarine et al., 2022, Stürner et al., 2022, DeAngelis et al., 2021, Dobramysl et al., 2021, King et al., 2021, Onur et al., 2021, Deng et al., 2020, Feuillette et al., 2020, Kiss et al., 2020, López-Gay et al., 2020, McNeill et al., 2020, Meltzer and Schuldiner, 2020.6.25, Ueoka et al., 2020, Wolterhoff et al., 2020, Bernardoni et al., 2019, Deshpande et al., 2019, Myat et al., 2019, Ohsawa, 2019, Yolland et al., 2019, Baranski et al., 2018, Forest et al., 2018, Gene Disruption Project members, 2018-, Green et al., 2018, Hao et al., 2018, Ikawa and Sugimura, 2018, Mortensen et al., 2018, Nithianandam and Chien, 2018, Pinheiro et al., 2017, Qu et al., 2017, Taylor et al., 2017, Yang and Veraksa, 2017, Becker et al., 2016, Bolin et al., 2016, Clandinin and Owens, 2016-, Harris, 2016.8.24, Sakuma et al., 2016, Segal et al., 2016, Squarr et al., 2016, Horan et al., 2015, Ashwal-Fluss et al., 2014, Bassett et al., 2014, Chen et al., 2014, Haack et al., 2014, Huelsmann, 2014.12.18, Nowotarsky and Peifer, 2014.10.31, Pézeron et al., 2014, Rudrapatna et al., 2014, Kwon et al., 2013, Kwon et al., 2013, Lucas et al., 2013, Newquist et al., 2013, Sano et al., 2012, Srinivasan et al., 2012, Takahashi et al., 2012, Becam et al., 2011, Choi et al., 2011, Friedman et al., 2011, Gervais and Casanova, 2011, Gonçalves-Pimentel et al., 2011, Kuzina et al., 2011, Tucker et al., 2011, Xiong and Rebay, 2011, Blanco et al., 2010, Gervais and Casanova, 2010, Gettings et al., 2010, Merdes, 2010.2.24, Song et al., 2010, Yu et al., 2010, Gates et al., 2009, Homem and Peifer, 2009, Johnson and Cagan, 2009, Lin et al., 2009, Nowotarski and Peifer, 2009.9.30, Dimitrova et al., 2008, FlyBase Curators, 2008-, Lyulcheva et al., 2008, Matusek et al., 2008, Prokopenko and Chia, 2008, Rezával et al., 2008, Song et al., 2008, Corrigall et al., 2007, Fox and Peifer, 2007, Gates et al., 2007, Joly et al., 2007, Prokopenko and Chia, 2007, Riedl et al., 2007, Stuart et al., 2007, Yang and Bashaw, 2006, Keleman et al., 2005, Li et al., 2005, Martin et al., 2005, Ayoob et al., 2004, Stanyon et al., 2004)

enb

(Solano et al., 2003, Shim et al., 2001)

l(2)02029

(McKim and Hayashi-Hagihara, 1998, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

Name Synonyms

Enabled

(Banerjee and Roy, 2018, Becker et al., 2016, Ducuing and Vincent, 2016, Parsons and Foley, 2016, Lucas et al., 2015, Vlisidou and Wood, 2015, Nowotarski et al., 2014, Winkelman et al., 2014, Huelsmann et al., 2013, Lucas et al., 2013, Puram and Bonni, 2013, Weavers and Skaer, 2013, Förster and Luschnig, 2012, Gates, 2012, Choi et al., 2011, Kuzina et al., 2011, Laplante and Nilson, 2011, Lee et al., 2011, Gervais and Casanova, 2010, Delon and Brown, 2009, Mulinari and Häcker, 2009, Alves-Silva et al., 2008, Nowotarski et al., 2008, Rogers et al., 2008, Santiago-Martínez et al., 2008, Santiago-Martínez et al., 2008, Song et al., 2008, Vernier and Stevens, 2008, Vincent et al., 2008, Corrigall et al., 2007, Pimentel et al., 2007, Herranz and Milan, 2006, Price et al., 2006, Wahlstrom et al., 2006, Martin et al., 2005, Gao and Bogert, 2003, Rogers et al., 2003, Bashaw et al., 2000)

VASP

(Lee et al., 2011)

enabled

(Rudrapatna et al., 2014, Becam et al., 2011, Choi et al., 2011, Gettings et al., 2010, Merdes, 2010.2.24, Sánchez-Soriano et al., 2010, Johnson and Cagan, 2009, Hoopfer et al., 2008, Prokopenko and Chia, 2008, Rezával et al., 2008, Bakal et al., 2007, Joly et al., 2007, Li et al., 2005, Bennett and Hoffmann, 1992, Gertler et al., 1990)

Secondary FlyBase IDs

FBgn0010503
FBgn0063438

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 80 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37201

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8T4F7

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8T4F7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4LFX0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8T4F7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000578

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37201/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000578

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000578

FlyMine - An integrated database for Drosophila genomics

FBgn0000578

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG15112

MARRVEL_MODEL - MARRVEL (model organism gene)

37201

Linkouts

BioGRID - A database of protein and genetic interactions.

62866

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0000578

DroID - A comprehensive database of gene and protein interactions.

FBgn0000578

DRSC - Results frm RNAi screens

FBgn0000578

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

5G2_anti-enabled

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ena_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000578

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0000578

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15112

Flygut - An atlas of the Drosophila adult midgut

FBgn0000578

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002504

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

37201

MIST (protein-protein) - An integrated Molecular Interaction Database

37201

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Cell-extracellular matrix interactions

References (418)

The following external sites may use different assemblies or annotations than FlyBase.

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