Human Disease Model Report: cancer, multiple, actin-remodeling regulators

General Information

Name

cancer, multiple, actin-remodeling regulators

FlyBase ID

FBhh0001148

Disease Ontology Term

cancer

Parent Disease

OMIM

Overview

Regulators of actin and cytoskeletal remodeling play a key role in orchestrating the cytoskeletal changes required for cell migration, in both normal and pathogenic contexts. Evidence is accumulating that, in addition to this function, actin remodeling regulators may participate in positive feedback networks that impact development of malignant phenotypes during the early stages of cellular transformation. Studies in Drosophila have contributed to understanding of these interactions.

Several slightly different models have been used; most use assays in wing discs with overexpression of Src64B or knockdown of Csk in discrete regions of the disc. Dmel\Src64B is the highest-scoring Drosophila ortholog of human gene SRC. Dmel\Csk is orthologous to the human CSK gene, a kinase that suppresses the activity of multiple genes, including the SRC proto-oncogene. Interacting genes were identified and characterized based on changes in the Src-related overproliferation phenotypes.

Actin modulators that have emerged as important in this process include ena (orthologous to human ENAH, EVL and VASP); tsr (orthologous to human CFL2, DSTN and CFL1); cpa (orthologous to human CAPZA1, CAPZA2 and CAPZA3); and cpb (orthologous to human CAPZB). A large-scale genetic screen has identified additional candidates.

The impact of overexpression of Dmel\ena has also been characterized in migrating embryonic macrophages (hemocytes) to address development of aggressive migratory behavior during cancer progression.

[updated Feb. 2021 by FlyBase; FBrf0222196]

Disease Summary Information

Disease Summary: cancer, multiple, actin-remodeling regulators

OMIM report

Human gene(s) implicated

Symptoms and phenotype

Genetics

Cellular phenotype and pathology

Molecular information

External links

Disease synonyms

cancer, epithelial, actin-remodeling regulators

cancer, epithelial, SRC with actin-remodeling regulators

Related Diseases

Related human health report(s)

cancer, epithelial, SRC,CSK-related

cancer, multiple, RAS-CSK(SRC)-related

cancer, multiple, RAS-SRC-related

cancer, multiple, RAS with actin regulators

Ortholog Information

Human gene(s) in FlyBase

Other mammalian ortholog(s) used

D. melanogaster Gene Information (6)

Dmel\Src64B

Gene Snapshot

Src oncogene at 64B (Src64B) encodes a Src family nonreceptor tyrosine kinase. It has many biological roles, including ring canal morphogenesis in oogenesis and the male germline, microfilament ring constriction during cellularization, and modulation of growth and apoptosis. Some of its roles overlap with those of the product of Src42A. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Highest-scoring ortholog of human gene SRC (many to many; multiple paralogs and orthologs in both species). Dmel\Src64B shares 50% identity and 67% similarity with human SRC.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\Csk

Gene Snapshot

C-terminal Src kinase (Csk) encodes a cytoplasmic tyrosine kinase that acts as a tumor suppressor through Src pathway inibition as well as a mediator of the activity of the product of Egfr. [Date last reviewed: 2019-03-07]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

High-scoring ortholog of human CSK; moderate-scoring ortholog of human MATK (1 Drosophila to 2 human); Dmel\Csk shares 62% identity and 75% similarity with the human CSK gene.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\ena

Gene Snapshot

enabled (ena) encodes the sole Drosophila member of the Ena/VASP family. It acts as a processive actin polymerase, stimulating actin addition at the barbed end. It has roles in both epithelial morphogenesis and CNS pathfinding. [Date last reviewed: 2019-03-07]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Moderate-scoring ortholog of human ENAH, EVL and VASP.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\tsr

Gene Snapshot

Contributions welcome

Molecular function (GO)

actin binding

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Moderate-scoring ortholog of human CFL2, DSTN and CFL1.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\cpb

Gene Snapshot

capping protein beta (cpb) encodes an actin-binding protein that, together with cpa, binds to the fast growing ends of actin filaments, restricting the addition or loss of actin monomers. It restricts JNK signaling, modulates Hippo signaling, maintains epithelial integrity and neuronal homeostasis, controls proliferation/apoptosis in wing imaginal discs, and promotes oogenesis. [Date last reviewed: 2018-09-13]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

DYNA-C
ACPC

Comments on ortholog(s)

High-scoring ortholog of human CAPZB.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\cpa

Gene Snapshot

capping protein alpha (cpa) encodes an actin-binding protein that, along with the product of cpb, binds to the fast growing ends of actin filaments to restrict the addition or loss of actin monomers. It limits the nuclear translocation of the Hippo pathway mediator encoded by yki and restricts JNK signaling. It maintains epithelial integrity and controls the balance between proliferation and apoptosis in the wing imaginal disc. It plays important roles during oogenesis, as well as maintaining neuronal homeostasis. [Date last reviewed: 2018-10-18]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

DYNA-C
ACPC

Comments on ortholog(s)

Moderate- to high-scoring ortholog of human CAPZA1, CAPZA2 and CAPZA3.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Other Genes Used: Viral, Bacterial, Synthetic (0)

Summary of Physical Interactions (91 groups)

Src64B

protein-protein

Interacting group

Assay

References

Src64B

enzymatic study, autoradiography

(Pedraza et al., 2004)

Src64B - 18w

anti tag coimmunoprecipitation, anti tag western blot

(Tamada et al., 2021)

Src64B - Cad96Ca

pull down, anti tag western blot, two hybrid

(Tsarouhas et al., 2014)

Src64B - CadN

pull down, anti tag western blot

(Pütz, 2019)

Src64B - Csk

enzymatic study, autoradiography

(Pedraza et al., 2004)

Src64B - drl

two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Petrova et al., 2013, Wouda et al., 2008)

Src64B - gcm2

two hybrid

(Hu et al., 2017.6.13)

Src64B - hzg

anti tag coimmunoprecipitation, multidimensional protein identification technology, western blot

(Nil et al., 2019)

Src64B - kirre

anti tag coimmunoprecipitation, western blot

(Tutor et al., 2014)

Src64B - stumps

anti bait coimmunoprecipitation, anti tag western blot

(Csiszar et al., 2010)

Src64B - Toll-6

anti tag coimmunoprecipitation, anti tag western blot

(Tamada et al., 2021)

Csk

protein-protein

Interacting group

Assay

References

Csk - ASPP

pull down, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Langton et al., 2007)

Csk - Gli

proximity ligation assay, fluorescence microscopy

(Samarasekera and Auld, 2018)

Csk - Src42A

enzymatic study, western blot, autoradiography

(Pedraza et al., 2004, Stewart et al., 2003)

Csk - Src64B

enzymatic study, autoradiography

(Pedraza et al., 2004)

Csk - wts

enzymatic study, western blot

(Stewart et al., 2003)

ena

protein-protein

Interacting group

Assay

References

ena

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Ahern-Djamali et al., 1999, Ahern-Djamali et al., 1998)

ena - Abi

pull down, anti tag western blot, western blot, far western blotting, peptide array

(Chen et al., 2014)

ena - Abl

enzymatic study, autoradiography, pull down, western blot, far western blotting, anti tag western blot, peptide massfingerprinting

(Ahern-Djamali et al., 1999, Wills et al., 1999, Ahern-Djamali et al., 1998, Comer et al., 1998, Gertler et al., 1995)

ena - Atg9

two hybrid

(Kiss et al., 2020)

ena - chic

two hybrid, anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Kiss et al., 2020, Matusek et al., 2008, Ahern-Djamali et al., 1999)

ena - DAAM

anti tag coimmunoprecipitation, anti tag western blot

(Matusek et al., 2008)

ena - dia

bimolecular fluorescence complementation, fluorescence microscopy, anti tag coimmunoprecipitation, western blot, pull down, two hybrid

(Bilancia et al., 2014, Homem and Peifer, 2009)

ena - drk

pull down, western blot

(Ahern-Djamali et al., 1999)

ena - ft

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

ena - Khc

two hybrid, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Martin et al., 2005)

ena - Lar

anti tag coimmunoprecipitation, western blot, pull down, enzymatic study, autoradiography

(Johnson et al., 2006, Wills et al., 1999)

ena - pico

two hybrid, anti tag coimmunoprecipitation, western blot

(Lyulcheva et al., 2008)

ena - Ptp10D

pull down, western blot

(Wills et al., 1999)

ena - Ptp69D

pull down, western blot

(Wills et al., 1999)

ena - robo1

pull down, western blot, anti bait coimmunoprecipitation, autoradiography, anti tag coimmunoprecipitation

(Fan et al., 2003, Bashaw et al., 2000)

ena - Rop

experimental knowledge based

(Guruharsha et al., 2011)

ena - SCAR

pull down, western blot

(Chen et al., 2014)

ena - Zyx

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, autoradiography

(Gaspar et al., 2015, Renfranz et al., 2003)

RNA-RNA

Interacting group

Assay

References

ena - mir-8

immunohistochemistry, fluorescence technology, necessary binding region

(Loya et al., 2014)

tsr

protein-protein

Interacting group

Assay

References

tsr - CG7737

experimental knowledge based

(Guruharsha et al., 2011)

tsr - flr

anti tag coimmunoprecipitation, western blot

(Chu et al., 2012)

tsr - HIP-R

experimental knowledge based

(Guruharsha et al., 2011)

tsr - mbt

enzymatic study, autoradiography

(Menzel et al., 2007)

tsr - Nlg2

anti tag coimmunoprecipitation, western blot, pull down, Identification by mass spectrometry

(Sun et al., 2023)

tsr - Rack1

anti tag coimmunoprecipitation, western blot

(Sun et al., 2023)

tsr - scrib

anti tag coimmunoprecipitation, western blot

(Cervantes-Sandoval et al., 2016)

tsr - Sumo

anti tag coimmunoprecipitation, comigration in sds page, anti tag western blot

(Pirone et al., 2017)

tsr - Synd

experimental knowledge based

(Guruharsha et al., 2011)

tsr - Tango2

experimental knowledge based

(Guruharsha et al., 2011)

tsr - tobi

experimental knowledge based

(Guruharsha et al., 2011)

tsr - yki

anti tag coimmunoprecipitation, western blot, bimolecular fluorescence complementation, fluorescence microscopy, proximity ligation assay

(Skouloudaki et al., 2019)

cpb

RNA-protein

Interacting group

Assay

References

cpb - stau

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

protein-protein

Interacting group

Assay

References

cpb - Act57B

experimental knowledge based

(Guruharsha et al., 2011)

cpb - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

cpb - Arp2

experimental knowledge based

(Guruharsha et al., 2011)

cpb - Arp3

experimental knowledge based

(Guruharsha et al., 2011)

cpb - Arp10

experimental knowledge based

(Guruharsha et al., 2011)

cpb - Arpc1

experimental knowledge based

(Guruharsha et al., 2011)

cpb - Arpc2

experimental knowledge based

(Guruharsha et al., 2011)

cpb - Arpc3A

experimental knowledge based

(Guruharsha et al., 2011)

cpb - Arpc4

experimental knowledge based

(Guruharsha et al., 2011)

cpb - Arpc5

experimental knowledge based

(Guruharsha et al., 2011)

cpb - CG7049

experimental knowledge based

(Guruharsha et al., 2011)

cpb - CG8578

experimental knowledge based

(Guruharsha et al., 2011)

cpb - cindr

anti bait coimmunoprecipitation, western blot, peptide massfingerprinting

(Johnson et al., 2008)

cpb - cpa

experimental knowledge based

(Guruharsha et al., 2011)

cpb - DCTN2-p50

experimental knowledge based

(Guruharsha et al., 2011)

cpb - DCTN4-p62

experimental knowledge based

(Guruharsha et al., 2011)

cpb - didum

experimental knowledge based

(Guruharsha et al., 2011)

cpb - dos

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

cpb - LRR

experimental knowledge based

(Guruharsha et al., 2011)

cpb - osk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Hurd et al., 2016)

cpb - Rheb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

cpb - Swip-1

experimental knowledge based

(Guruharsha et al., 2011)

cpa

protein-protein

Interacting group

Assay

References

cpa - Act57B

experimental knowledge based

(Guruharsha et al., 2011)

cpa - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

cpa - Arp2

experimental knowledge based

(Guruharsha et al., 2011)

cpa - Arp3

experimental knowledge based

(Guruharsha et al., 2011)

cpa - Arpc1

experimental knowledge based

(Guruharsha et al., 2011)

cpa - Arpc2

experimental knowledge based

(Guruharsha et al., 2011)

cpa - Arpc3A

experimental knowledge based

(Guruharsha et al., 2011)

cpa - Arpc4

experimental knowledge based

(Guruharsha et al., 2011)

cpa - Arpc5

experimental knowledge based

(Guruharsha et al., 2011)

cpa - CG8578

experimental knowledge based

(Guruharsha et al., 2011)

cpa - cindr

anti bait coimmunoprecipitation, peptide massfingerprinting

(Johnson et al., 2008)

cpa - cpb

experimental knowledge based

(Guruharsha et al., 2011)

cpa - DCTN2-p50

experimental knowledge based

(Guruharsha et al., 2011)

cpa - didum

experimental knowledge based

(Guruharsha et al., 2011)

cpa - dos

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

cpa - Dsor1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

cpa - LRR

experimental knowledge based

(Guruharsha et al., 2011)

cpa - Ndc80

anti tag coimmunoprecipitation, peptide massfingerprinting

(Przewloka et al., 2007)

cpa - osk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Hurd et al., 2016)

cpa - Rheb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

cpa - S6k

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

cpa - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

cpa - Swip-1

experimental knowledge based

(Guruharsha et al., 2011)

Alleles Reported to Model Human Disease (Disease Ontology) (32 alleles)

Src64B

Models Based on Experimental Evidence ( 4 )

Allele

Disease

Evidence

References

Src64B^YF.UAS

model of kidney disease

CEA

(Tutor et al., 2014)

Src64B^UAS.cCa

model of cancer

CEA

(Enomoto et al., 2021)

CEA with Ras85D^V12.UAS

(Enomoto et al., 2021)

model of colorectal cancer

CEA

(Cordero et al., 2014)

model of intestinal cancer

CEA

(Nászai et al., 2021)

Src64B^UY1332

model of carcinoma

CEA

(Pagarigan et al., 2013)

Src64B^UAS.cUa

model of intestinal cancer

CEA

(Kohlmaier et al., 2015)

Modifiers Based on Experimental Evidence ( 8 )

Allele

Disease

Interaction

References

Src64B^GL00297

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Src64B^GD12263

ameliorates cancer

modeled by Ras85D^V12.UAS

(Ma et al., 2013)

Src64B^G7151

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Src64B^UAS.cCa

model of cancer

is ameliorated by Delta^DN.UAS

(Enomoto et al., 2021)

is ameliorated by Delta^RevF10

(Enomoto et al., 2021)

is ameliorated by N^ECN.UAS

(Enomoto et al., 2021)

is ameliorated by N^GD14477

(Enomoto et al., 2021)

is exacerbated by N^ICN.UAS

(Enomoto et al., 2021)

is ameliorated by shg^UAS.sgGFP

(Enomoto et al., 2021)

is exacerbated by zfh1^UAS.cPa

(Enomoto et al., 2021)

is ameliorated by Delta^DN.UAS

(Enomoto et al., 2021)

model of colorectal cancer

is ameliorated by Egfr^DN.UAS

(Cordero et al., 2014)

is ameliorated by Ras85D^KK108029

(Cordero et al., 2014)

is ameliorated by Ras85D^GD12553

(Cordero et al., 2014)

is ameliorated by Hsap\MATK^UAS.cVa

(Cordero et al., 2014)

model of intestinal cancer

is ameliorated by Rala^KK108989

(Nászai et al., 2021)

Src64B^JF03234

ameliorates cancer

modeled by Hsap\CDH3^UASp.cPa

(Faria et al., 2023)

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Src64B^UY1332

model of carcinoma

is ameliorated by PRL-1^UAS.cPa

(Pagarigan et al., 2013)

Src64B^HMC03327

ameliorates cancer

modeled by Hsap\CDH3^UASp.cPa

(Faria et al., 2023)

ameliorates lung adenocarcinoma

modeled by Hsap\KIF5B::Hsap\RET^UAS.cDa

(Das and Cagan, 2017)

Src64B^UAS.cUa

model of intestinal cancer

is ameliorated by Stat92E^RNAi.UAS.cUa

(Kohlmaier et al., 2015)

is ameliorated by Egfr^RNAi.UAS.cUa

(Kohlmaier et al., 2015)

is ameliorated by Egfr^RNAi.UAS.cUa

(Kohlmaier et al., 2015)

is ameliorated by Stat92E^RNAi.UAS.cUa

(Kohlmaier et al., 2015)

Csk

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Csk^unspecified

model of cancer

CEA with Nek2^UAS.GFP

(Das et al., 2013)

Csk^Q156Stop

model of cancer

CEA with Ras85D^V12.UAS

(Hirabayashi et al., 2013)

model of carcinoma

CEA with Ras85D^V12.UAS

(Hirabayashi and Cagan, 2015)

CEA with InR^A1325D.UAS, Ras85D^V12.UAS

(Hirabayashi and Cagan, 2015)

Modifiers Based on Experimental Evidence ( 4 )

Allele

Disease

Interaction

References

Csk^unspecified

model of cancer

is exacerbated by Nek2^UAS.GFP

(Das et al., 2013)

Csk^j1D8

exacerbates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

Csk^Q156Stop

exacerbates cancer

modeled by Ras85D^V12.UAS

(Hirabayashi et al., 2013)

Csk^c04256

exacerbates viral infectious disease

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates infectious mononucleosis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates Epstein-Barr virus hepatitis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

ena

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 6 )

Allele

Disease

Interaction

References

ena²¹⁰

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

ena²³

ameliorates chronic myeloid leukemia

modeled by Hsap\ABL1::Hsap\BCR^UAS.cBa

(Bernardoni et al., 2019)

ena^GD8910

ameliorates chronic myeloid leukemia

modeled by Hsap\ABL1::Hsap\BCR^UAS.cBa

(Bernardoni et al., 2019)

ena^KK107752

ameliorates chronic myeloid leukemia

modeled by Hsap\ABL1::Hsap\BCR^UAS.cBa

(Bernardoni et al., 2019)

ena^HMS01953

exacerbates autism spectrum disorder

modeled by Abca3^GD3708

(Ueoka et al., 2020)

ena^{UASp.B.mCherry}

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-231.UAS}

(Onur et al., 2021)

tsr

Models Based on Experimental Evidence ( 7 )

Allele

Disease

Evidence

References

tsr^HMS00534

model of nemaline myopathy 7

CEC

(Christophers et al., 2024)

CEA

(Balakrishnan et al., 2020)

tsr^N121

model of alcohol use disorder

CEA with RhoGAP18B¹

(Ojelade et al., 2015)

tsr^N96A

model of nemaline myopathy 7

CEA

(Balakrishnan et al., 2020)

model of autism spectrum disorder

CEA

(Sun et al., 2023)

tsr^S3A.UAS

model of alcohol use disorder

CEA

(Ojelade et al., 2015)

tsr^k05633

model of alcohol use disorder

CEA with RhoGAP18B¹

(Ojelade et al., 2015)

tsr^KK108706

model of nemaline myopathy 7

CEA

(Balakrishnan et al., 2020)

tsr^{V7M.UAS.Tag:HA}

model of nemaline myopathy 7

CEA

(Balakrishnan et al., 2020)

Modifiers Based on Experimental Evidence ( 7 )

Allele

Disease

Interaction

References

tsr^HMS00534

model of nemaline myopathy 7

is ameliorated by PI31^UAS.Tag:HA

(Balakrishnan et al., 2020)

is exacerbated by Prosβ6^1.B.UAS

(Balakrishnan et al., 2020)

tsr^N121

ameliorates hereditary systemic amyloidosis 1

modeled by Hsap\TTR^{V30M.UAS.Tag:HA}

(Oliveira da Silva et al., 2020)

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-231.UAS}

(Onur et al., 2021)

tsr^S3A.UAS

ameliorates Parkinson's disease 1

modeled by Hsap\SNCA^QUAS.cOa

(Sarkar et al., 2021)

tsr^{UAS.Tag:polyHis}

ameliorates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Fulga et al., 2007)

ameliorates Parkinson's disease 8

modeled by Lrrk^e03680

(Sarkar et al., 2021)

tsr^UAS.cNa

ameliorates Parkinson's disease 1

modeled by Hsap\SNCA^QUAS.cOa

(Sarkar et al., 2021)

tsr^UAS.EGFP

ameliorates autism spectrum disorder

modeled by Nlg2^KO70

(Sun et al., 2023)

tsr^S3A.UAS.EGFP

ameliorates autism spectrum disorder

modeled by Nlg2^KO70

(Sun et al., 2023)

cpa

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

cpa^69E

model of developmental disorder of mental health

CEA with cpa^107E

(Huang et al., 2020)

cpa^107E

model of developmental disorder of mental health

CEA with cpa^69E

(Huang et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Alleles Representing Disease-Implicated Variants

Genetic Tools, Stocks and Reagents

Sources of Stocks

Contact lab of origin for a reagent not available from a public stock center.

Bloomington Stock Center Disease Page

Related mammalian, viral, bacterial, or synthetic transgenes

Allele

Transgene

Publicly Available Stocks

Selected Drosophila transgenes

Allele

Transgene

Publicly Available Stocks

PBac{UAS-tsr.S3A.EGFP}

tsr^UAS.cNa

P{UAS-tsr.N}

P{UAS-tsr.N}1.7, y¹ w^*; βTub60D^Pin-1/CyO

tsr^S3A.UAS

P{UAS-tsr.S3A}

P{UAS-tsr.S3A}4.1, y¹ w^*; βTub60D^Pin-1/CyO

tsr^{UAS.Tag:polyHis}

P{UAS-tsr.His}

ena^{UASp.B.mCherry}

P{UASp-mCherry.ena}

w^*; P{UASp-mCherry.ena}attP2

w^*; P{UAS-Src64B.C}2

Src64B^YF.UAS

P{UAS-Src64B.YF}

y¹ w^*; P{UAS-Src64B.YF}121/CyO

RNAi constructs available

Allele

Transgene

Publicly Available Stocks

y¹ v¹; P{TRiP.JF01597}attP2

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02249}attP2

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00534}attP2

w¹¹¹⁸; P{GD9299}v45668

cpb^JF02068

P{TRiP.JF02068}

y¹ v¹; P{TRiP.JF02068}attP2

cpb^HMS02349

P{TRiP.HMS02349}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02349}attP2

cpb^HMJ21056

P{TRiP.HMJ21056}

y¹ v¹; P{TRiP.HMJ21056}attP40/CyO

w¹¹¹⁸; P{GD8910}v43056

y¹ v¹; P{TRiP.JF01155}attP2

ena^HMS01953

P{TRiP.HMS01953}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01953}attP2

Csk^GD15975

P{GD15975}

w¹¹¹⁸; P{GD15975}v48282

Csk^GD9345

P{GD9345}

w¹¹¹⁸; P{GD9345}v32877

y¹ sc^* v¹ sev²¹; P{TRiP.GL00044}attP2

Csk^HMS02277

P{TRiP.HMS02277}

y¹ v¹; P{TRiP.HMS02277}attP40/CyO

Src64B^RNAi.UAS

P{UAS-Src64B.RNAi}

w^*; P{UAS-Src64B.RNAi}6.1

Src64B^GD12263

P{GD12263}

w¹¹¹⁸ P{GD12263}v35252

Src64B^JF03234

P{TRiP.JF03234}

y¹ v¹; P{TRiP.JF03234}attP2

Src64B^GL00297

P{TRiP.GL00297}

y¹ sc^* v¹ sev²¹; P{TRiP.GL00297}attP2

y¹ v¹; P{TRiP.HMC03327}attP40

Src64B^HMC05164

P{TRiP.HMC05164}

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05164}attP40

y¹ v¹; P{TRiP.JF01597}attP2

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02249}attP2

Selected Drosophila classical alleles

Allele

Allele class

Mutagen

Publicly Available Stocks

cpa^KO

amorphic allele - molecular evidence

CRISPR/Cas9

w^*; cpa^KO

cpa^69E

ethyl methanesulfonate

cpa^107E

ethyl methanesulfonate

tsr^k05633

P-element activity

y¹ w^67c23; P{lacW}tsr^k05633/CyO

tsr^N121

loss of function allele

P-element activity

w^*; P{FRT(w^hs)}G13 tsr^N121/CyO

tsr^N96A

loss of function allele

P-element activity

y¹ w^*; P{FRT(w^hs)}G13 tsr^N96A/CyO

tsr^Δ96

loss of function allele

cpb^KO

amorphic allele - molecular evidence

CRISPR/Cas9

w^*; cpb^KO/CyO

ena^Δ151

amorphic allele - molecular evidence

CRISPR/Cas9

ena²³

ena²³/CyO, P{act-lacZ.B}CB1

ena⁴⁶

amorphic allele - genetic evidence

ethyl methanesulfonate

ena²¹⁰

wg^Sp-1 Bl¹ ena²¹⁰/CyO

ena^C14-06

amorphic allele - molecular evidence

ethyl methanesulfonate

Csk^S030003

loss of function allele

Delta2-3 transposase

Csk^S017909

loss of function allele

Delta2-3 transposase

Csk^j1D8

loss of function allele

P-element activity

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{3'lacW}j1D8/TM6B, P{Car20y}TPN1, Tb¹

Csk^c04256

piggyBac activity

w¹¹¹⁸; PBac{PB}Csk^c04256

Csk^Q156Stop

ethyl methanesulfonate

Csk^unspecified

Src64B^UY1332

P-element activity

y¹ w^67c23; P{Mae-UAS.6.11}Src64B^UY1332

Src64B^ko

amorphic allele - genetic evidence

ends-out gene targeting

w^*; Src42A^E1/CyO; TI{TI}Src64B^ko/TM6B, Tb⁺

Src64B^G7151

P-element activity

y¹ w^*; P{EP}Src64B^G7151 CG32246^G7151

cpa^KO

amorphic allele - molecular evidence

CRISPR/Cas9

w^*; cpa^KO

cpa^69E

ethyl methanesulfonate

cpa^107E

ethyl methanesulfonate

References (6)

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