FlyBase Gene Report: Dmel\PolD3

General Information

Symbol

Dmel\PolD3

Species

D. melanogaster

Name

DNA polymerase delta subunit 3

Annotation Symbol

CG3975

Feature Type

protein_coding_gene

FlyBase ID

FBgn0283467

Gene Model Status

Current

Stock Availability

27 publicly available

Gene Summary

DNA polymerase delta subunit 3 (PolD3) encodes a non-essential subunit of the multi-subunit DNA polymerases delta and zeta. It has roles during embryonic DNA replication and promotes several types of DNA repair, including homologous recombination repair of double-strand breaks. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

BG:DS07295.3 , pol32, rd, reduced

Key Links

Genomic Location

Cytogenetic map

35C5-35C5

Sequence location

2L:15,254,819..15,256,304 [-]

Recombination map

2-51

RefSeq locus

NT_033779 REGION:15254819..15256304

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables DNA polymerase processivity factor activity

inferred from mutant phenotype

(Kane et al., 2012)

enables protein binding

inferred from physical interaction with UniProtKB:P54358,UniProtKB:Q9W088

(Ji et al., 2019)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in DNA repair

inferred from mutant phenotype

(Tritto et al., 2015)

involved_in DNA synthesis involved in double-strand break repair via homologous recombination

inferred from mutant phenotype

(Kane et al., 2012)

involved_in double-strand break repair via break-induced replication

inferred from mutant phenotype

(Bhandari et al., 2019)

involved_in double-strand break repair via homologous recombination

inferred from mutant phenotype

(Ciesielski et al., 2015)

involved_in mitotic DNA replication

inferred from mutant phenotype

(Tritto et al., 2015)

involved_in protein-containing complex localization

inferred from mutant phenotype

(Ji et al., 2019)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

part_of delta DNA polymerase complex

inferred from direct assay

(Ji et al., 2019)

located_in nucleoplasm

inferred from direct assay

(Ji et al., 2019)

located_in nucleus

inferred from direct assay

(Ji et al., 2019)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of zeta DNA polymerase complex

inferred from sequence or structural similarity with UniProtKB:Q15054

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

DNA POLYMERASE DELTA

DNA POLYMERASE ZETA

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

DNA POLYMERASE DELTA -: DNA polymerase delta functions as a leading strand polymerase during nuclear DNA synthesis. It is a multi-subunit enzyme in which DNApol-δ encodes the catalytic subunit with polymerase and 3'-5' exonuclease proofreading activities. (Adapted from PMID:30005324.)
DNA POLYMERASE ZETA -: DNA polymerase zeta is one of several translesion synthesis polymerases that act to promote replication through DNA lesions that would otherwise stall the replicative polymerases. It is a heterotetramer, in which the catalytic subunit is encoded by DNApol-ζ, and is specialized for the extension step of lesion bypass. (Adapted from PMID:30005324.)

Protein Function (UniProtKB)

Accessory component of the DNA polymerase delta complex and possibly the DNA polymerase zeta complex (By similarity). As a component of the delta complex, participates in high fidelity genome replication, including lagging strand synthesis, DNA recombination and repair (PubMed:22532806, PubMed:25826374, PubMed:31100062). Required to recruit the DNA polymerase delta complex to the nucleus of rapidly dividing embryonic cells, and as a consequence is essential for genome replication during the earliest cell cycles (PubMed:25826374, PubMed:31100062). Increases the efficiency and processivity of DNA synthesis of the DNA polymerases during mitotic DNA replication and repair (PubMed:22532806, PubMed:25826374, PubMed:31100062). During development this function is essential for preventing replication stress that results in the formation of chromosomal fragile sites (CFS) such as chromosomal breaks (PubMed:25826374, PubMed:31100062). Ensures genomic stability by promoting several types of DNA repair mechanisms including repairing broken dicentric chromosomes through homolog-dependent break-induced replication (BIR) (PubMed:22532806, PubMed:25826374, PubMed:31053594). During homologous recombination (HR) repair, required for maintaining the processivity of the delta complex during break-induced replication; a form of HR that requires extensive DNA synthesis such as the repair of large gaps (PubMed:22532806, PubMed:25826374). Able to suppress position effect variegation and may therefore have a role in the induction of chromatin state changes that likely include its activities in DNA replication and repair (PubMed:25826374).

(UniProt, Q9Y118)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

rd: reduced

rd: reduced

From Bridges and Brehme, 1944, Carnegie Inst. Washington Publ. No. 552: 157.

rds: reduced-scraggly

Edith M. Wallace, unpublished.

Bristles reduced in length and thickness, sometimes twisted; best seen on sternopleurals; male more extreme than female (rd1); female may have abnormal abdominal banding (rds). Males fertile, but females may be sterile (rd1) (Lynch, 1919, Genetics 4: 501-33).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\PolD3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9Y118)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

PolD3-RB

FBtr0303164

NM_001201887

1486

431

PolD3-RC

FBtr0336864

NM_135899

1391

431

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

PolD3-PB

FBpp0292263

47.0

431

9.98

FBpp0307818

47.0

431

9.98

Q9Y118

NP_609743

AAF53445

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

431 aa isoforms: PolD3-PB, PolD3-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of both the DNA polymerase delta and DNA polymerase zeta complexes (By similarity). The DNA polymerase delta complex consists of three subunits: the catalytic subunit PolD1 and two accessory subunits PolD2/Pol31 and PolD3/Pol32 (PubMed:31100062). Within the delta complex, interacts with both PolD1 and PolD2 (PubMed:31100062). Component of the DNA polymerase zeta complex consisting of four subunits: the catalytic subunit PolZ1 and three accessory subunits PolZ2/Rev7, PolD2/Pol31 and PolD3/Pol32 (By similarity).

(UniProt, Q9Y118)

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9Y118

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\PolD3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pol32_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.79

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of delta DNA polymerase complex

inferred from direct assay

(Ji et al., 2019)

located_in nucleoplasm

inferred from direct assay

(Ji et al., 2019)

located_in nucleus

inferred from direct assay

(Ji et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\PolD3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\PolD3

View exonic expression by tissue for Dmel\PolD3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027559

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34892/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0283467

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027559

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0283467

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3975

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 19 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of PolD3

Transgenic constructs containing regulatory region of PolD3

Aberrations (Deficiencies and Duplications) ( 157 )

Inferred from experimentation ( 157 )

Gene not disrupted in

Df(2L)64j

(Furukawa et al., 1992, Ashburner et al., 1990, Ashburner, 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)A47

(Ashburner et al., 1982)

Df(2L)A63

(Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A72

(Ashburner et al., 1990, Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A178

(Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A217

(Ashburner et al., 1982)

Df(2L)A220

(Ashburner et al., 1982)

Df(2L)A245

(Ashburner et al., 1982)

Df(2L)A260

(Ashburner et al., 1982)

Df(2L)A264

(Ashburner et al., 1982)

Df(2L)A266

(Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A267

(Ashburner et al., 1990, Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A379

(Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A400

(Ashburner, 1982, Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A445

(Ashburner et al., 1982)

Df(2L)Adh-nBR105

(Roote, 1996.1.11, Roote, 1996.8)

Df(2L)Adh-nBR122

(Roote, 1996.1.11, Roote, 1996.8)

Df(2L)Adh-nBR128

(Roote, 1996.8)

Df(2L)Adh-nBR131

(Roote, 1996.1.11, Roote, 1996.8)

Df(2L)ARR1

(Furukawa et al., 1992, Ashburner et al., 1990, Ashburner, 1982)

Df(2L)b75

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)b80e3

(Ashburner et al., 1982)

Df(2L)b81a1

(Ashburner et al., 1982)

Df(2L)b84a3

(Furukawa et al., 1992)

Df(2L)b84a7

(Furukawa et al., 1992)

Df(2L)b84h50

(Furukawa et al., 1992)

Df(2L)b88c58

(Roote, 1996.8, Alexandrov and Alexandrova, 1991)

Df(2L)el77

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)fn1

(O'Donnell et al., 1977)

Df(2L)fn2

(Grau et al., 1984, Ashburner et al., 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn3

(Ashburner, 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn5

(Furukawa et al., 1992, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn7

(Ashburner, 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn12

(Ashburner et al., 1990, Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn15

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn30

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn31

(Ashburner, 1982, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn36

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)H20

(Ashburner et al., 1990)

Df(2L)k07245-mr32

(Roote, 2000.6.13)

Df(2L)nNxF1

(Roote, 1996.8)

Df(2L)osp144

(Ashburner et al., 1982)

Df(2L)PA4

(Ashburner et al., 1983)

Df(2L)PM1

(Caggese et al., 1994)

Df(2L)PZ06430-mr14

(Roote, 2000.6.13)

Df(2L)r10

(Ashburner et al., 1990)

Df(2L)RA5

(Ashburner et al., 1990)

Df(2L)RM5

(Ashburner et al., 1990)

Df(2L)RN2

(Ashburner et al., 1990)

Df(2L)Sco^rv4

(Ashburner et al., 1990, Ashburner et al., 1983, Woodruff and Ashburner, 1979)

Df(2L)Sco^rv10

(Grau et al., 1984, Ashburner et al., 1983, Woodruff and Ashburner, 1979)

Df(2L)Sco^rv14

(Ashburner et al., 1983, Woodruff and Ashburner, 1979)

Df(2L)Sco^rv16

(Ashburner et al., 1990, Grau et al., 1984, Ashburner et al., 1983)

Df(2L)Sco^rv18

(Ashburner et al., 1990, Ashburner et al., 1983)

Df(2L)Sco^rv19

(Ashburner et al., 1990, Grau et al., 1984, Ashburner et al., 1983)

Df(2L)Sco^rv25

(Ashburner et al., 1990, Grau et al., 1984, Ashburner et al., 1983)

Df(2L)TE35B-3

(Gubb et al., 1985)

Df(2L)TE35B-4

(Gubb et al., 1985)

Df(2L)TE35B-5

(Gubb et al., 1985)

Df(2L)TE35B-6

(Gubb et al., 1985)

Df(2L)TE35B-7

(Gubb et al., 1985)

Df(2L)TE35B-8

(Gubb et al., 1985)

Df(2L)TE35B-9

(Gubb et al., 1985)

Df(2L)TE35B-10

(Gubb et al., 1985)

Df(2L)TE35B-11

(Gubb et al., 1985)

Df(2L)TE35B-12

(Gubb et al., 1985)

Df(2L)TE35B-15

(Gubb et al., 1985)

Df(2L)TE35BC-1

(Gubb et al., 1984, Ashburner, 1982, Ashburner et al., 1981)

Df(2L)TE35BC-6

(Gubb et al., 1984)

Df(2L)TE35BC-7

(Furukawa et al., 1992, Gubb et al., 1984)

Df(2L)TE35BC-23

(Gubb et al., 1984)

Df(2L)TE35BC-28

(Furukawa et al., 1992, Gubb et al., 1984)

Df(2L)TE35BC-29

(Gubb et al., 1984)

Df(2L)TE35BC-31

(Gubb et al., 1984)

Df(2L)TE35D-1

(Ashburner et al., 1990)

Df(2L)TE35D-2

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-3

(Ashburner et al., 1990)

Df(2L)TE35D-13

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-14

(Ashburner et al., 1990)

Df(2L)TE35D-19

(Ashburner et al., 1990)

Df(2L)TE35D-21

(Ashburner et al., 1990)

Df(2L)TE35D-22

(Ashburner et al., 1990)

Df(2L)W

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)75c^LC158.1^R

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)C158^LSco^rv11R

(Ashburner et al., 1983)

In(2L)C158^LSco^rv17R

(Ashburner et al., 1983)

In(2LR)pkD1-rv8

(Roote, 1997.6.6)

In(2LR)Sco^rv1-DV7

(Ashburner et al., 1990)

T(2;3)Sco^rv7

(Ashburner et al., 1990, Ashburner and Harrington, 1984, Grau et al., 1984, Ashburner et al., 1983, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

T(Y;2)A80^DR15^P

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

T(Y;2)J165^DP58^P

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Ts(2Lt;3Lt)TE35BC-3+Ts(2Rt;3Rt)Sco^rv13

(Ashburner et al., 1983)

Ts(Y;2Lt)el⁴+Ts(YSt;2Rt)R15

(Ashburner et al., 1982, Ashburner et al., 1981)

Ts(YSt;2Lt)G74+Ts(YSt;2Rt)A80

(Woodruff and Ashburner, 1979)

Ts(YSt;2Lt)G74+Ts(YSt;2Rt)R15

(Woodruff and Ashburner, 1979)

Gene disrupted in

Df(2L)A48

(Grau et al., 1984, Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A215

(Ashburner et al., 1982)

Df(2L)A246

(Ashburner et al., 1982)

Df(2L)A263

(Furukawa et al., 1992, Ashburner et al., 1982)

Df(2L)A376

(Ashburner, 1982, Ashburner et al., 1982)

Df(2L)A377

(Ashburner, 1982, Ashburner et al., 1982)

Df(2L)Adh-nBR128

(Roote, 1996.1.11)

Df(2L)b80e4

(Ashburner et al., 1990)

Df(2L)do1

(Ashburner, 1982, Ashburner et al., 1981)

Df(2L)el20

(Ashburner et al., 1990)

Df(2L)el28

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)el80f1

(Ashburner et al., 1983)

Df(2L)Exel7062

(Ryder, 2004.4.26)

Df(2L)fn1

(Grau et al., 1984, Ashburner et al., 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)fn26

(Ashburner et al., 1990, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn27

(Ashburner et al., 1990, Ashburner, 1982, Ashburner et al., 1982, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)n78l3

(Ashburner et al., 1990, Ashburner et al., 1982)

Df(2L)noc11

(Ashburner et al., 1990)

Df(2L)osp29

(Furukawa et al., 1992, Grau et al., 1984, Ashburner et al., 1982)

Df(2L)osp38

(Ashburner et al., 1990)

Df(2L)PolD3^NR42

(Tritto et al., 2015)

Df(2L)PolD3^R2

(Tritto et al., 2015)

Df(2L)rd9

(Roote, 1996.8, Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)Sco-1

(Ashburner, 1982)

Df(2L)Sco-2

(Gubb et al., 1984)

Df(2L)Sco7

(Ashburner et al., 1990, Ashburner et al., 1983, Ashburner et al., 1981)

Df(2L)Sco^rv4

(Woodruff and Ashburner, 1979)

Df(2L)Sco^rv23

(Grau et al., 1984, Ashburner et al., 1983, Ashburner, 1982)

Df(2L)SS1

(Ashburner et al., 1990, Grau et al., 1984)

Df(2L)TE35B-1

(Gubb et al., 1985)

Df(2L)TE35B-2

(Gubb et al., 1985)

Df(2L)TE35B-14

(Roote, 1996.8)

Df(2L)TE35BC-3

(Ashburner et al., 1990, Grau et al., 1984, Gubb et al., 1984, Ashburner, 1982, Ashburner et al., 1982)

Df(2L)TE35BC-4

(Ashburner et al., 1990, Gubb et al., 1984, Ashburner, 1982, Ashburner et al., 1982)

Df(2L)TE35BC-8

(Gubb et al., 1984)

Df(2L)TE35BC-24

(Gubb et al., 1984)

Df(2L)TE35BC-34

(Ashburner et al., 1990, Gubb et al., 1984)

Df(2L)TE35BC-35

(Gubb et al., 1984)

Df(2L)TE35D-4

(Ashburner et al., 1990)

Df(2L)TE35D-6

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-15

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-16

(Ashburner et al., 1990)

Df(2L)TE35D-18

(Furukawa et al., 1992)

Df(2L)TE35D-20

(Ashburner et al., 1990)

Df(2L)TE35D-23

(Ashburner et al., 1990)

In(2L)75c

(Grau et al., 1984, Ashburner, 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)C158^LSco^rv11R

(Ashburner and Harrington, 1984, Ashburner et al., 1981)

In(2L)C158^LSco^rv17R

(Ashburner and Harrington, 1984)

In(2L)dpp^s22Ldpp^d36R

(Ashburner et al., 1990)

In(2L)el18

(Ashburner et al., 1990)

In(2L)TE35B-13

(Gubb et al., 1985)

In(2LR)noc^4LSco^rv9R

(Ashburner and Harrington, 1984)

In(2LR)TE35B-6^LSco^rv9R

(Ashburner and Harrington, 1984)

T(Y;2)R15^DJ165^P

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Tp(2;2)A446

(Ashburner et al., 1982, Ashburner et al., 1982)

Ts(2Lt;3Lt)TE35B-4+Ts(2Rt;3Rt)Sco^rv13

(Ashburner and Harrington, 1984)

Ts(2Lt;3Lt)TE35BC-3+Ts(2Rt;3Rt)Sco^rv13

(Ashburner and Harrington, 1984)

Ts(YLt;2Lt)G74+Ts(YSt;2Rt)B210

(Woodruff and Ashburner, 1979)

Ts(YSt;2Lt)G74+Ts(YSt;2Rt)J165

(Woodruff and Ashburner, 1979)

Ts(YSt;2Lt)G74+Ts(YSt;2Rt)P58

(Woodruff and Ashburner, 1979)

Gene duplicated in

Dp(2;1)Sco^rv23

(Ashburner et al., 1990, Grau et al., 1984, Ashburner et al., 1983, Ashburner, 1982, Ashburner et al., 1981)

Dp(2;2)TE35B-54^L

(Ashburner et al., 1990)

Dp(2;3)osp³

(Ashburner et al., 1990)

In(2L)C163.41^LC158^R

(Ashburner et al., 1990, Ashburner, 1982)

In(2L)Sco^rv17LC158^R

(Ashburner et al., 1990, Ashburner, 1982)

Gene not duplicated in

Dp(2;2)Adh3

(Ashburner, 1982)

Dp(2;2)GYL

(Ashburner et al., 1990, Ashburner, 1982)

Dp(2;2)GYS

(Ashburner, 1982)

Gene partially disrupted in

Df(2L)PolD3^NR42

(Tritto et al., 2015)

Df(2L)PolD3^R2

(Tritto et al., 2015)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | maternal effect

PolD3^L30

lethal - all die during embryonic stage | non-rescuable maternal effect (with PolD3^NR42)

PolD3^R2

lethal - all die during embryonic stage | non-rescuable maternal effect (with PolD3^R2)

PolD3^NR42

viable

PolD3^EY15283

Sterility

Allele

female fertile

PolD3^rd-s

female fertile (with PolD3^rd-4)

PolD3^rd-s

female fertile (with PolD3^rd-s)

PolD3^rd-4

female sterile

female sterile (with PolD3^NR42)

PolD3^R2

female sterile (with PolD3^R2)

PolD3^NR42

fertile

PolD3^EY15283

male fertile

male fertile (with PolD3^NR42)

PolD3^R2

male fertile (with PolD3^R2)

PolD3^NR42

Other Phenotypes

Allele

abnormal DNA repair

PolD3^L2

abnormal size | adult stage

PolD3^L30

chemical sensitive

PolD3^L2

chemical sensitive (with PolD3^NR42)

PolD3^R2

chemical sensitive (with PolD3^R2)

PolD3^NR42

majority die during embryonic stage | rescuable maternal effect (with PolD3^R2)

majority die during embryonic stage | rescuable maternal effect (with PolD3^rd-1)

majority die during embryonic stage | rescuable maternal effect (with PolD3^rd-s)

radiation sensitive

PolD3^L2

radiation sensitive (with PolD3^NR42)

PolD3^R2

radiation sensitive (with PolD3^R2)

PolD3^NR42

suppressor of variegation (with PolD3^NR42), with In(1)w^m4h

PolD3^R2

suppressor of variegation (with PolD3^R2), with In(1)w^m4h

PolD3^NR42

visible (with PolD3^NR42)

PolD3^R2

visible (with PolD3^R2)

PolD3^NR42

Phenotype manifest in

Allele

abdomen | female

PolD3^rd-s

abdominal tergite (with PolD3^NR42)

PolD3^R2

abdominal tergite (with PolD3^R2)

PolD3^NR42

chaeta (with PolD3^NR42)

PolD3^R2

chaeta (with PolD3^R2)

chaeta (with PolD3^rd-1)

PolD3^R2

chaeta (with PolD3^rd-s)

PolD3^R2

chromosome | third instar larval stage (with PolD3^L27)

PolD3^L30

chromosome | third instar larval stage (with PolD3^L30)

PolD3^L27

embryonic neuroblast | third instar larval stage (with PolD3^L27)

PolD3^L30

embryonic neuroblast | third instar larval stage (with PolD3^L30)

PolD3^L27

macrochaeta

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\POLD3

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Pold3

1 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Pold3

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (1)

Drer\pold3

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP008352

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\POLD3

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\cdc27

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:PolD3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

PolD3 - Nelf-E

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

PolD3 - PolD1

anti bait coimmunoprecipitation, western blot

(Ji et al., 2019)

PolD3 - PolD2

anti bait coimmunoprecipitation, western blot

(Ji et al., 2019)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

PolD3

enhanceable

Pif1

(Kocak et al., 2019)

enhanceable

(Ji et al., 2019)

enhanceable

(Kocak et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9Y118 )

Linkouts

MIST (protein-protein) - An integrated Molecular Interaction Database

34892

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-51

Cytogenetic map

35C5-35C5

Sequence location

2L:15,254,819..15,256,304 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35C5-35C5

Limits computationally determined from genome sequence between P{EP}vig^EP812&P{PZ}stc⁰⁵⁴⁴¹ and P{EP}esg^EP633&P{PZ}esg⁰⁷⁰⁸²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-51

(FlyBase, 2016)

2-48.2

(Comeron et al., 2012)

2-51.3 +/- 0.2

(O'Donnell et al., 1977)

2-[51]

Left of (cM)

Right of (cM)

(Woodruff and Ashburner, 1979)

Notes

Recombinants of gft⁰⁶⁴³⁰ indicate that the gene order is rd, l(2)35Cc, gft.

(Ashburner et al., 1999.10.12)

Stocks and Reagents

Stocks (27)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

PolD3^rd-1/SM5

603931

w¹¹¹⁸; P{SX4.lexA::HG}PolD3^S5-14/CyO

21125

y¹ w^67c23; P{EPgy2}PolD3^EY15283

67358

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06464}attP40

3179

Adh^UF Rnmt⁴ PolD3^rd-s pr¹ cn¹/CyO

3181

Adh^UF Ankrd49⁴ PolD3^rd-s pr¹ cn¹/CyO

Kyoto

101416

CyO / w¹b³ Adhⁿ¹ PolD3^rd-s pr¹ sn¹

105837

Adc^b-1 elA¹ PolD3^rd-s pr¹ cn¹ or^49h

NIG-Fly

3975R-2

P{NIG.3975R}2

M2L-1492

y¹ w¹¹¹⁸; PolD3^SK1 P{neoFRT}40A/CyO-Tb

M2L-1493

y¹ w¹¹¹⁸; PolD3^SK7 P{neoFRT}40A/CyO-Tb

2LG-1219

y² VhaAC39-1^cho-1 v¹; P{U6-PolD3.NIG.sgRNA}attP40/CyO

More stocks available...

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH08865

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0283467

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

EC051459

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Ji et al., 2019)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: Pol32 rd

(Tritto et al., 2015)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: BG:DS07295.3 CG3975

(Kodira, 1999.11)

Source for identity of: Pol32 CG3975

(Kane et al., 2012)

Source for identity of: PolD3 Pol32

(Marygold et al., 2020)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

BG:DS07295.3

(Giot et al., 2003, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12)

BcDNA:GH08865

CG3975

(National Institute of Genetics Fly Stocks, 2022-, Meadows, 2019.11.20, Çiçek et al., 2016, Tritto et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Kane et al., 2012, Keleman et al., 2009.8.5, Perkins et al., 2009, Moorman et al., 2006, Proschel et al., 2006, Burgler and Macdonald, 2005)

Pol32

(Gerlach and Herranz, 2020, Ji et al., 2019, Kane et al., 2012)

PolD3

(Yamaguchi and Cotterill, 2023, Marygold et al., 2020)

pol32

(Bhandari et al., 2019, Kocak et al., 2019, Alexander et al., 2016, Tritto et al., 2015)

(Tritto et al., 2015, Roote, 2008.8.22, Pasyukova et al., 2004)

Name Synonyms

DNA polymerase delta subunit 3

(Marygold et al., 2020)

Pol32 polymerase delta subunit

reduced

(Pasyukova et al., 2004)

Secondary FlyBase IDs

FBgn0027559
FBgn0027887
FBgn0003213

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34892

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9Y118

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9Y118

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9Y118

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027559

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34892/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0283467

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0283467

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3975

MARRVEL_MODEL - MARRVEL (model organism gene)

34892

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0283467

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pol32_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027559

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0283467

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3975

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

34892

References (75)

The following external sites may use different assemblies or annotations than FlyBase.

0.79

Report Sections