FlyBase Allele Report: Scer\GAL4[sim.PS]

General Information

Symbol

Scer\GAL4^sim.PS

Species

S. cerevisiae

Name

single-minded promoter construct of Scholz

FlyBase ID

FBal0058755

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-sim.S}

Also Known As

sim-GAL4, simGAL4, simGAL, sim::Gal4, single-minded-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Scholz et al., 1997, Scholz et al., 1997, Golembo et al., 1996)

Progenitor genotype

Carried in construct

P{GAL4-sim.S}

Cytology

Description

3.5kb EcoRV sim promoter fragment regulates expression of a GAL4 driver.

(Scholz et al., 1997, Scholz et al., 1997)

Allele components

Component

Use(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 17

ventral midline of embryo

(Scholz et al., 1997, Golembo et al., 1996)

embryonic stage 10 -- 15

embryonic foregut | anterior | ventral

(Page, 2003)

embryonic stage 11

ventral midline of embryo

(Yan and Lin, 2007)

embryonic stage 11 -- 13

ventral midline of embryo

(Bossing et al., 2012)

embryonic stage 15

midline glial cell

(Scholz et al., 1997)

Additional Information

Statement

Reference

Drives expression in cells of the ventral midline.

(FlyBase Curators, 2017)

In embryos, from stage 9 on, expression in all cells of the ventral midline; late in embryonic development, expression most prominent in midline glial cells.

(Scholz et al., 1997)

In embryo, expression exclusively in the ventral midline, but appears to initiate later than wild-type sim expression.

(Golembo et al., 1996)

Marker for

ventral midline of embryo

(Bossing et al., 2012, Scholz et al., 1997)

Reflects expression of

sim

(Yan and Lin, 2007, Page, 2003, Scholz et al., 1997)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with en^UAS.cYa

(Bossing and Brand, 2006)

abnormal neuroanatomy, with Ephrin^UAS.cBa

(Bossing and Brand, 2002)

abnormal neuroanatomy, with Ephrin^UAS.Tag:MYC

(Boyle et al., 2006)

abnormal neuroanatomy, with tutl^AT02763.UAS

(Al-Anzi and Wyman, 2009)

abnormal neuroanatomy, with tutl^GH15753.UAS

(Al-Anzi and Wyman, 2009)

abnormal neuroanatomy, with Wnt5^UAS.cFa

(Ding et al., 2011)

abnormal neuroanatomy | embryonic stage, with PlexB^{EcTM.UAS.Tag:MYC}

(Wu et al., 2011, Wu et al., 2011)

decreased cell death, with NT1^Cterm.UAS

(Zhu et al., 2008)

decreased cell death, with NT1^Cysknot.UAS

(Zhu et al., 2008)

decreased occurrence of cell division | embryonic stage 13, with hep^CA.UAS

(Barros and Bossing, 2021)

decreased occurrence of cell division | embryonic stage 13, with IKKε^DN.UAS

(Barros and Bossing, 2021)

increased cell death, with hh^UAS.cIa

(Bossing and Brand, 2006)

increased cell death | embryonic stage 17, with Df(3L)ED4342, NT1^{RNAi.pwCK.UAS}

(Zhu et al., 2008)

increased cell death | embryonic stage 17, with NT1⁴¹, NT1^RNAi.CK.UAS

(Zhu et al., 2008)

increased cell death | embryonic stage 17, with NT1⁴¹, NT1^RNAi.pro.UAS

(Zhu et al., 2008)

increased cell death | embryonic stage 17, with NT1⁴¹, NT1^{RNAi.pwCK.UAS}

(Zhu et al., 2008)

increased occurrence of cell division | embryonic stage 13, with dl^UAS.cHa

(Barros and Bossing, 2021)

increased occurrence of cell division | embryonic stage 13, with Hsap\MAPT^UAS.0N3R.cFa

(Barros and Bossing, 2021)

increased occurrence of cell division | embryonic stage 13, with Hsap\MAPT^UAS.cWa

(Barros and Bossing, 2021)

increased occurrence of cell division | embryonic stage 13, with Tl^{10b.UAS.Tag:MYC}

(Barros and Bossing, 2021)

increased occurrence of cell division | embryonic stage 13, with Traf6^UAS.cSa

(Barros and Bossing, 2021)

increased occurrence of cell division | embryonic stage 13, with αTub67C^UASp.GFP

(Barros and Bossing, 2021)

increased occurrence of cell division | embryonic stage, with rho^UAS.cdCa

(Page, 2003)

increased occurrence of cell division | embryonic stage, with αTub67C^UASp.GFP

(Bossing et al., 2012)

increased occurrence of cell division | embryonic stage, with βTub56D^UASp.GFP

(Bossing et al., 2012)

Phenotype Manifest In

axon, with futsch^EP1419

(Hummel et al., 2000)

denticle, with wg^UAS.cLa

(Desbordes and Sanson, 2003)

embryonic/first instar larval cuticle, with wg^UAS.cLa

(Desbordes and Sanson, 2003)

embryonic/larval ganglionic tracheal branch 1 | embryonic stage, with rho^UAS.cBa

(Gallio et al., 2004)

embryonic/larval ganglionic tracheal branch, with bnl^UAS.cSa

(Araújo and Casanova, 2011)

embryonic/larval hemocyte, with Pvf2^d02444

(Wood et al., 2006)

embryonic central brain, with rho^UAS.cdCa

(Page, 2003)

embryonic somatic muscle cell, with kuz^DN.UAS

(Schimmelpfeng et al., 2001)

embryonic somatic muscle cell | lateral, with Df(3R)DG4, sr^UAS.cBa

(Becker et al., 1997)

intermediate longitudinal fascicle, with PlexB^{EcTM.UAS.Tag:MYC}

(Wu et al., 2011, Wu et al., 2011)

larval anterior commissure, with en^UAS.cYa

(Bossing and Brand, 2006)

larval anterior commissure, with Wnt5^EY03178

(Yoshikawa et al., 2003)

larval anterior commissure, with Wnt5^UAS.cFa

(Ding et al., 2011, Fradkin et al., 2004)

larval DA1 motor neuron, with spi^s.UAS

(Menne et al., 1997)

larval intersegmental nerve branch ISNd of A1-7, with tutl^AT02763.UAS

(Al-Anzi and Wyman, 2009)

larval intersegmental nerve branch ISNd of A1-7, with tutl^GH15753.UAS

(Al-Anzi and Wyman, 2009)

larval longitudinal connective, with aop^ACT.UAS

(Scholz et al., 1997, Scholz et al., 1997)

larval longitudinal connective, with kuz^DN.UAS

(Schimmelpfeng et al., 2001)

larval longitudinal connective, with tutl^AT02763.UAS

(Al-Anzi and Wyman, 2009)

larval longitudinal connective, with tutl^GH15753.UAS

(Al-Anzi and Wyman, 2009)

larval MP1 neuron, with en^UAS.cYa

(Bossing and Brand, 2006)

larval neuron, with Ephrin^UAS.cBa

(Bossing and Brand, 2002)

larval posterior commissure, with Dscam1^UAS.cWa, Scer\GAL4^eg-Mz360

(Wojtowicz et al., 2004)

larval posterior commissure, with en^UAS.cYa

(Bossing and Brand, 2006)

larval somatic muscle cell | ventral, with sr^b.UAS

(Vorbruggen and Jackle, 1997)

larval thoracic perisympathetic organ, with spi^s.UAS

(Luer et al., 1997)

larval transverse nerve, with tutl^AT02763.UAS

(Al-Anzi and Wyman, 2009)

larval transverse nerve, with tutl^GH15753.UAS

(Al-Anzi and Wyman, 2009)

larval ventral nerve cord, with Cnx99A^EP3522

(McGovern et al., 2003)

larval ventral nerve cord | embryonic stage, with Pvr^DN.UASp

(Olofsson and Page, 2005)

larval ventral nerve cord commissure, with Cnx99A^EP3522

(McGovern et al., 2003)

larval ventral nerve cord commissure, with ich^GS-A304

(McGovern et al., 2003)

larval ventral nerve cord commissure, with Rac1^V12.UAS

(Hummel et al., 2000)

larval ventral nerve cord commissure, with shi^K44A.UAS

(Onel et al., 2004)

larval VUM neuron, with Hem^UAS.cHa

(Hummel et al., 2000)

larval VUM neuron, with Rac1^V12.UAS

(Hummel et al., 2000)

medial longitudinal fascicle, with Cnx99A^EP3522

(McGovern et al., 2003)

midline glial cell, with en^UAS.cYa

(Bossing and Brand, 2006)

midline glial cell, with pnt^P1.UAS

(Scholz et al., 1997, Scholz et al., 1997)

midline glial cell | ectopic, with BacA\p35^UAS.cHa

(Giesen et al., 2003)

midline neuronal primordium, with en^UAS.cYa

(Bossing and Brand, 2006)

midline primordium, with αTub67C^UASp.GFP

(Bossing et al., 2012)

midline primordium, with βTub56D^UASp.GFP

(Bossing et al., 2012)

MP1 tract, with PlexB^{EcTM.UAS.Tag:MYC}

(Wu et al., 2011)

pCC neuron, with spi^s.UAS

(Menne et al., 1997)

presumptive embryonic/larval central nervous system, with Ephrin^UAS.Tag:MYC

(Boyle et al., 2006)

presumptive embryonic/larval central nervous system, with kuz^DN.UAS

(Schimmelpfeng et al., 2001)

symmetrical commissure, with aop^ACT.UAS

(Scholz et al., 1997, Scholz et al., 1997)

symmetrical commissure, with Egfr^DN.UAS

(Scholz et al., 1997, Scholz et al., 1997)

symmetrical commissure, with Ephrin^UAS.cBa

(Bossing and Brand, 2002)

symmetrical commissure, with Ephrin^UAS.Tag:MYC

(Boyle et al., 2006)

symmetrical commissure, with Ephrin^{ΔIC.UAS.Tag:MYC}

(Boyle et al., 2006)

symmetrical commissure, with Jra^Asp.UAS

(Scholz et al., 1997, Scholz et al., 1997)

symmetrical commissure, with tutl^AT02763.UAS

(Al-Anzi and Wyman, 2009)

symmetrical commissure, with tutl^GH15753.UAS

(Al-Anzi and Wyman, 2009)

tracheal section, with bnl^UAS.cSa

(Dorfman et al., 2002)

UMI interneuron & axon, with en^UAS.cYa

(Bossing and Brand, 2006)

ventral midline of embryo, with hh^UAS.cIa

(Bossing and Brand, 2006)

ventral midline of embryo | embryonic stage 13, with dl^UAS.cHa

(Barros and Bossing, 2021)

ventral midline of embryo | embryonic stage 13, with hep^CA.UAS

(Barros and Bossing, 2021)

ventral midline of embryo | embryonic stage 13, with Hsap\MAPT^UAS.0N3R.cFa

(Barros and Bossing, 2021)

ventral midline of embryo | embryonic stage 13, with Hsap\MAPT^UAS.cWa

(Barros and Bossing, 2021)

ventral midline of embryo | embryonic stage 13, with IKKε^DN.UAS

(Barros and Bossing, 2021)

ventral midline of embryo | embryonic stage 13, with IKKε^UAS.Tag:MYC

(Barros and Bossing, 2021)

ventral midline of embryo | embryonic stage 13, with Tl^{10b.UAS.Tag:MYC}

(Barros and Bossing, 2021)

ventral midline of embryo | embryonic stage 13, with Traf6^UAS.cSa

(Barros and Bossing, 2021)

ventral midline of embryo | embryonic stage 13, with αTub67C^UASp.GFP

(Barros and Bossing, 2021)

ventral midline of embryo | embryonic stage 13 | conditional, with hep^CA.UAS

(Barros and Bossing, 2021)

VUM neuron & axon, with en^UAS.cYa

(Bossing and Brand, 2006)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

αTub67C^UASp.GFP, Scer\GAL4^sim.PS has increased occurrence of cell division | embryonic stage 13 phenotype, enhanceable by IKKε^DN.UAS, Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

NOT Enhanced by

Statement

Reference

Scer\GAL4^sim.PS, Wnt5^UAS.cFa has abnormal neuroanatomy phenotype, non-enhanceable by uzip^D43

(Ding et al., 2011)

Suppressed by

Statement

Reference

αTub67C^UASp.GFP, Scer\GAL4^sim.PS has increased occurrence of cell division | embryonic stage 13 phenotype, suppressible by IKKε^UAS.Tag:MYC, Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

Scer\GAL4^sim.PS, dl^UAS.cHa has increased occurrence of cell division | embryonic stage 13 phenotype, suppressible by Scer\GAL4^sim.PS/hep^CA.UAS

(Barros and Bossing, 2021)

NOT suppressed by

Statement

Reference

Scer\GAL4^sim.PS, Wnt5^UAS.cFa has abnormal neuroanatomy phenotype, non-suppressible by uzip^D43

(Ding et al., 2011)

Enhancer of

Statement

Reference

IKKε^DN.UAS, Scer\GAL4^sim.PS is an enhancer of increased occurrence of cell division | embryonic stage 13 phenotype of αTub67C^UASp.GFP, Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

Suppressor of

Statement

Reference

Scer\GAL4^sim.PS/hep^CA.UAS is a suppressor of increased occurrence of cell division | embryonic stage 13 phenotype of Scer\GAL4^sim.PS, dl^UAS.cHa

(Barros and Bossing, 2021)

IKKε^UAS.Tag:MYC, Scer\GAL4^sim.PS is a suppressor of increased occurrence of cell division | embryonic stage 13 phenotype of αTub67C^UASp.GFP, Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

NOT Suppressor of

Statement

Reference

sli^UAS.cKa/Scer\GAL4^sim.PS is a non-suppressor of abnormal neuroanatomy phenotype of ptc^unspecified

(Merianda et al., 2005)

sli^UAS.cKa, Scer\GAL4^sim.PS, Scer\GAL4^UAS.cHa is a non-suppressor of abnormal neuroanatomy phenotype of ptc^unspecified

(Merianda et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

αTub67C^UASp.GFP, Scer\GAL4^sim.PS has ventral midline of embryo | embryonic stage 13 phenotype, enhanceable by IKKε^DN.UAS, Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

Scer\GAL4^sim.PS, bnl^UAS.cSa has tracheal section phenotype, enhanceable by sr^UAS.cBa

(Dorfman et al., 2002)

NOT Enhanced by

Statement

Reference

Scer\GAL4^sim.PS, Wnt5^UAS.cFa has larval anterior commissure phenotype, non-enhanceable by uzip^D43

(Ding et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^sim.PS, dl^UAS.cHa has ventral midline of embryo | embryonic stage 13 phenotype, suppressible by Scer\GAL4^sim.PS/hep^CA.UAS

(Barros and Bossing, 2021)

αTub67C^UASp.GFP, Scer\GAL4^sim.PS has ventral midline of embryo | embryonic stage 13 phenotype, suppressible by IKKε^UAS.Tag:MYC, Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

Ephrin^UAS.Tag:MYC, Scer\GAL4^sim.PS has symmetrical commissure phenotype, suppressible by Eph^x652

(Boyle et al., 2006)

Scer\GAL4^sim.PS, Wnt5^UAS.cFa has larval anterior commissure phenotype, suppressible by por^PB16

(Fradkin et al., 2004)

Scer\GAL4^sim.PS, Wnt5^EY03178 has larval anterior commissure phenotype, suppressible by drl^Red2

(Yoshikawa et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^sim.PS, Wnt5^UAS.cFa has larval anterior commissure phenotype, non-suppressible by uzip^D43

(Ding et al., 2011)

Enhancer of

Statement

Reference

IKKε^DN.UAS, Scer\GAL4^sim.PS is an enhancer of ventral midline of embryo | embryonic stage 13 phenotype of αTub67C^UASp.GFP, Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

Scer\GAL4^sim.PS/BacA\p35^UAS.cHa is an enhancer of midline glial cell | ectopic phenotype of dib^unspecified

(Giesen et al., 2003)

Suppressor of

Statement

Reference

Scer\GAL4^sim.PS/hep^CA.UAS is a suppressor of ventral midline of embryo | embryonic stage 13 phenotype of Scer\GAL4^sim.PS, dl^UAS.cHa

(Barros and Bossing, 2021)

IKKε^UAS.Tag:MYC, Scer\GAL4^sim.PS is a suppressor of ventral midline of embryo | embryonic stage 13 phenotype of αTub67C^UASp.GFP, Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

rho^UAS.cBa/Scer\GAL4^sim.PS is a suppressor of embryonic/larval ganglionic tracheal branch phenotype of ru^unspecified

(Gallio et al., 2004)

Scer\GAL4^sim.PS/NetB^UAS.cHa is a suppressor of larval ventral nerve cord commissure | embryonic stage phenotype of Iqsec^U112

(Onel et al., 2004)

Rac1^V12.UAS/Scer\GAL4^sim.PS is a suppressor of larval ventral nerve cord commissure phenotype of Hem^J4-48

(Hummel et al., 2000)

Rac1^V12.UAS/Scer\GAL4^sim.PS is a suppressor of larval longitudinal connective phenotype of Hem^J4-48

(Hummel et al., 2000)

Mmus\Sox2^UAS.cSa/Scer\GAL4^sim.PS is a suppressor of larval ventral nerve cord commissure phenotype of D^r72/Df(3L)fz-GS1a

(Sanchez Soriano and Russell, 1998)

Mmus\Sox2^UAS.cSa/Scer\GAL4^sim.PS is a suppressor of larval longitudinal connective phenotype of D^r72/Df(3L)fz-GS1a

(Sanchez Soriano and Russell, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

tutl^GH15753.UAS/Scer\GAL4^sim.PS rescues tutl^ex383

(Al-Anzi and Wyman, 2009)

tutl^AT02763.UAS/Scer\GAL4^sim.PS rescues tutl^ex383

(Al-Anzi and Wyman, 2009)

Scer\GAL4^sim.PS/tutl^HL01565.UAS rescues tutl^ex383

(Al-Anzi and Wyman, 2009)

tutl^LD28224.UAS/Scer\GAL4^sim.PS rescues tutl^ex383

(Al-Anzi and Wyman, 2009)

tutl^GH08133.UAS/Scer\GAL4^sim.PS rescues tutl^ex383

(Al-Anzi and Wyman, 2009)

tutl^GH15753.UAS/Scer\GAL4^sim.PS rescues Df(2L)ed-dp/tutl^ex383

(Al-Anzi and Wyman, 2009)

tutl^AT02763.UAS/Scer\GAL4^sim.PS rescues Df(2L)ed-dp/tutl^ex383

(Al-Anzi and Wyman, 2009)

Scer\GAL4^sim.PS/tutl^HL01565.UAS rescues Df(2L)ed-dp/tutl^ex383

(Al-Anzi and Wyman, 2009)

tutl^LD28224.UAS/Scer\GAL4^sim.PS rescues Df(2L)ed-dp/tutl^ex383

(Al-Anzi and Wyman, 2009)

tutl^GH08133.UAS/Scer\GAL4^sim.PS rescues Df(2L)ed-dp/tutl^ex383

(Al-Anzi and Wyman, 2009)

Iqsec^P2.UAS/Scer\GAL4^sim.PS rescues Iqsec^U112

(Onel et al., 2004)

Iqsec^P1.UAS/Scer\GAL4^sim.PS rescues Iqsec^U112

(Onel et al., 2004)

Partially rescues

seq^UAS.cBa/Scer\GAL4^sim.PS partially rescues seq^Z1241

(Araújo and Casanova, 2011)

seq^UAS.cBa/Scer\GAL4^sim.PS partially rescues seq^C022/seq^Z1241

(Araújo and Casanova, 2011)

seq^UAS.cBa/Scer\GAL4^sim.PS partially rescues seq^C022

(Araújo and Casanova, 2011)

Hem^UAS.cHa/Scer\GAL4^sim.PS partially rescues Hem^J4-48

(Hummel et al., 2000)

Scer\GAL4^sim.PS/D^UAS.cSa partially rescues D^r72/Df(3L)fz-GS1a

(Sanchez Soriano and Russell, 1998)

D^ΔC75.UAS/Scer\GAL4^sim.PS partially rescues D^r72/Df(3L)fz-GS1a

(Sanchez Soriano and Russell, 1998)

sim^UAS.cMa/Scer\GAL4^sim.PS partially rescues sim²

(Menne et al., 1997)

Fails to rescue

kermit^UAS.Tag:HA/Scer\GAL4^sim.PS fails to rescue kermit^ex31

(Chak and Kolodkin, 2014)

Scer\GAL4^sim.PS/β-Spec^UAS.cHa fails to rescue β-Spec^unspecified

(Hülsmeier et al., 2007)

Wnt5^UAS.cFa/Scer\GAL4^sim.PS fails to rescue Wnt5⁴⁰⁰

(Fradkin et al., 2004)

Sdc^UAS.cSa/Scer\GAL4^sim.PS fails to rescue Sdc^unspecified

(Steigemann et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^sim.PS

Scer\GAL4^sim.PS

Name Synonyms

single-minded promoter construct of Scholz

(Golembo et al., 1996)

Secondary FlyBase IDs

References (52)

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