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FB2026_01
,
released March 12, 2026
dPAK2, Ste20, Pak2
PAK kinase - downstream effector of Cdc42 - regulates cell adhesion dependent processes during photoreceptor cell differentiation
Please see the JBrowse view of Dmel\mbt for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.42
Gene model reviewed during 5.50
There is only one protein coding transcript and one polypeptide associated with this gene
Interacts tightly with GTP-bound but not GDP-bound Cdc42 and weakly with Rac1.
Autophosphorylated when activated by Cdc42.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mbt using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
In interphase neuroblasts, either weak cortical localization of mbt or a more pronounced basal staining was detected. Accumulation of apical mbt protein started at prophase, reached a peak during metaphase and decreased during anaphase. Thus, the apical enrichment of mbt is cell cycle dependent. This cell cycle dependent asymmetric accumulation of mbt was also observed in embryonic brain neuroblasts and thus seems to be present throughout neural development.
JBrowse - Visual display of RNA-Seq signals
View Dmel\mbt in JBrowse1-54
1-55.7
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
New stable cell line derived from S2-unspecified : A stable cell line that expresses shg under the control of the Act5C promoter was established. Stable S2R+ cell lines expressing inducible wild type mbt (Tag:MYC-Mbt), a kinase-dead version (Tag:MYC-Mbt[T525A]) and a constitutively-activated mbt (Tag:MYC-Mbt[S492N/S521E]) were used.
Candidate stable intronic sequence RNA (sisRNA) identified within CDS of this gene.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells and/or aneuploidy, an increase in the proportion of G2/M phase cells and chromosome alignment defects are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
mbt could be part of a general mechanism regulating cell number in a variety of neuronal tissues.
Mutations in mbt primarily interfere with the generation or survival of the intrinsic cells (Kenyon cells) of the mushroom body.
Source for merge of: mbt anon- EST:fe1G7
Source for merge of: mbt Pak2
