Human Disease Model Report: obesity, susceptibility to (postulated), hedgehog signaling pathway

General Information

Name

obesity, susceptibility to (postulated), hedgehog signaling pathway

FlyBase ID

FBhh0000944

Disease Ontology Term

obesity

Parent Disease

obesity, susceptibility to (fly models overview)

OMIM

Overview

In a large-scale screen for obesity susceptibility genes in Drosophila, a number of candidate genes proved to be members of or regulators of the hedgehog signaling pathway. RNAi-effected knockdown of these genes specifically in fat body (but not in neurons, muscle, or oenoctyes) results in significant changes in measured triglyceride levels. Genes identified include the hedgehog ligand (hh), the binding protein ihog, the coreceptor smoothened (smo), the coactivator nejire (nej), the downstream transcription factor cubits interuptus (ci), the hedgehog target and positive regulator Sxl, and the negative regulator Su(fu).

See also the FlyBase report for the Hedgehog Signaling Pathway (FBgg0000978).

[updated Jan. 2019 by FlyBase; FBrf0222196]

Disease Summary Information

Parent Disease Summary: obesity, susceptibility to (fly models overview)

Symptoms and phenotype

Obesity is an abnormal accumulation of body fat, usually 20% or more over an individual's ideal body weight. Obesity is associated with increased risk of illness, disability, and death. (http://medical-dictionary.thefreedictionary.com/obesity).

(FlyBase Curators, 2013-)

The development of obesity is recognized as having both genetic and environmental components (https://www.sciencelearn.org.nz/resources/203-obesity-genetic-or-environmental).

(FlyBase Curators, 2013-)

Specific Disease Summary: obesity, susceptibility to (postulated), hedgehog signaling pathway

OMIM report

Human gene(s) implicated

Symptoms and phenotype

Genetics

Cellular phenotype and pathology

Molecular information

External links

Disease synonyms

Related Diseases

Related human health report(s)

obesity, susceptibility to (fly models overview)

Related Specific Diseases

OMIM phenotypic series

Disease

Associated Human gene(s)

Drosophila model

Human transgene in Drosophila

Ortholog Information

Human gene(s) in FlyBase

Other mammalian ortholog(s) used

D. melanogaster Gene Information (7)

Dmel\hh

Gene Snapshot

hedgehog (hh) encodes the Hh signaling pathway ligand. It acts as a morphogen contributing to segment polarity determination, stem cells maintenance and cell migration. Post-translational modifications of the product of hh are essential for its restrictive spreading and signaling activity. [Date last reviewed: 2019-03-07]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

High-scoring ortholog of human SHH, DHH, and IHH (3 human to 1 Drosophila).

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\ihog

Gene Snapshot

interference hedgehog (ihog) encodes a type 1 membrane protein that acts as a co-receptor for the product of hh upstream or at the level of the receptor encoded by ptc. It interacts with the products of disp, dlp, dally and shf. It is involved in imaginal pattern formation and embryonic cuticle pattern formation. [Date last reviewed: 2018-11-15]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

HH-SMOC

Comments on ortholog(s)

Moderate-scoring ortholog of human BOC and CDON (2 human to 2 Drosophila); the other Drosophila gene is boi.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\smo

Gene Snapshot

smoothened (smo) encodes a critical component of the Hedgehog signaling pathway. It is regulated by phosphorylation, dimerization, and cell-surface accumulation upon Hedgehog stimulation. The product of smo is also regulated by ubiquitin and other small molecules such as phospholipids. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

SMO
HH-SMOC

Comments on ortholog(s)

High-scoring ortholog of human SMO (1 human to 1 Drosophila).

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\nej

Gene Snapshot

nejire (nej) encodes the transcriptional co-activator CBP. It acetylates several nuclear proteins, including the histone encoded by His3 on K18, K27, and H4 on K8. By regulating gene expression, the product of nej has roles in cell proliferation, cell signaling and differentiation, and in developmental patterning. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

High-scoring ortholog of human EP300 and CREBBP (2 human to 1 Drosophila).

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\ci

Gene Snapshot

cubitus interruptus (ci) encodes a Zn-finger family of transcription factor. It contributes to Hedgehog (Hh) signaling pathway that is involved in pattern formation and growth control. The product of ci undergoes limited proteolysis to be converted into a truncated form that function as a transcription repressor in the absence of the ligand encoded by hh but is converted into a full-length transcription activator in the presence of the product of hh. [Date last reviewed: 2019-03-07]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

High-scoring ortholog of human GLI3 and GLI2; moderate-scoring ortholog of GLI1 (3 human to 1 Drosophila).

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\Sxl

Gene Snapshot

Sex lethal (Sxl) encodes an RNA-binding protein, member of the RNA recognition motif (RRM) family. It is a master-feminizing switch contributing to all aspects of sexual dimorphism. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

HH-SMOP

Comments on ortholog(s)

Low-scoring ortholog of human RBMS1, RBMS2, RBMS3; the Drosophila gene shep is more closely related to all 3 human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\Su(fu)

Gene Snapshot

Suppressor of fused (Su(fu)) encodes a negative regulator of the Hedgehog signaling pathway. It forms a complex with the transcription factor encoded by ci to inhibit its activity. [Date last reviewed: 2019-09-26]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

HH-SMON

Comments on ortholog(s)

High-scoring ortholog of human SUFU (1 human to 1 Drosophila).

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Other Genes Used: Viral, Bacterial, Synthetic (0)

Summary of Physical Interactions (187 groups)

protein-protein

Interacting group

Assay

References

molecular sieving, molecular weight, molecular weight estimation by staining, anti tag coimmunoprecipitation, western blot

(Manikowski et al., 2020, Schürmann et al., 2018, Gallet et al., 2006)

hh - apolpp

anti bait coimmunoprecipitation, anti tag western blot, western blot, cosedimentation

(Eugster et al., 2007, Panakova et al., 2005)

hh - Arf6

anti bait coimmunoprecipitation, western blot

(Chabu et al., 2017)

hh - boi

pull down, anti tag western blot, western blot

(Zheng et al., 2010, Yao et al., 2006)

hh - CG5504

anti bait coimmunoprecipitation, western blot

(Canamasas et al., 2003)

hh - dally

anti tag coimmunoprecipitation, western blot

(Eugster et al., 2007)

hh - disp

anti bait coimmunoprecipitation, anti tag western blot, electron microscopy, microscale thermophoresis

(Cannac et al., 2020, Callejo et al., 2011)

hh - dlp

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot

(Yan et al., 2010)

hh - Hrs

cosedimentation, western blot

(Parchure et al., 2015)

hh - ihog

x-ray crystallography, predetermined participant, molecular sieving, cosedimentation, colocalization, fluorescence microscopy, inferred by author, surface plasmon resonance, affinity technology, tag visualisation, pull down, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot, anti tag western blot

(Simon et al., 2021, Kim et al., 2011, Williams et al., 2010, Zheng et al., 2010, Zhang et al., 2007, McLellan et al., 2006, Yao et al., 2006)

hh - ptc

pull down, anti tag western blot, affinity technology, fluorescence microscopy, inferred by author, anti bait coimmunoprecipitation, western blot, cross-linking study, tag visualisation

(Zheng et al., 2010, Lu et al., 2006, Canamasas et al., 2003, Wesley, 1999)

hh - Ptr

anti tag coimmunoprecipitation, western blot

(Bolatto et al., 2022)

hh - rasp

enzymatic study, autoradiography

(Hardy and Resh, 2012)

hh - shf

anti tag coimmunoprecipitation, western blot

(Gorfinkiel et al., 2005)

hh - Vps28

cosedimentation, western blot

(Parchure et al., 2015)

ihog

RNA-RNA

Interacting group

Assay

References

ihog - mir-7

fluorescence technology, luminiscence technology, necessary binding region, quantitative reverse transcription pcr

(Da Ros et al., 2013)

protein-protein

Interacting group

Assay

References

ihog

anti tag coimmunoprecipitation, anti tag western blot, bimolecular fluorescence complementation, fluorescence microscopy, molecular sieving, predetermined participant, cosedimentation

(Yang et al., 2021, Wu et al., 2019, McLellan et al., 2006)

ihog - cher

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Lalioti et al., 2025)

ihog - dally

colocalization, fluorescence microscopy, inferred by author

(Simon et al., 2021)

ihog - dlp

colocalization, fluorescence microscopy, inferred by author

(Simon et al., 2021)

ihog - hh

(Simon et al., 2021, Kim et al., 2011, Williams et al., 2010, Zheng et al., 2010, Zhang et al., 2007, McLellan et al., 2006, Yao et al., 2006)

ihog - mys

anti tag coimmunoprecipitation, anti tag western blot

(Qi et al., 2023)

ihog - ptc

anti bait coimmunoprecipitation, anti tag western blot, western blot

(Yan et al., 2010, Zheng et al., 2010)

ihog - Rab8

anti tag coimmunoprecipitation, western blot

(Gore et al., 2021)

ihog - shf

anti tag coimmunoprecipitation, anti tag western blot

(Bilioni et al., 2013)

ihog - vn

enzyme linked immunosorbent assay

(Ozkan et al., 2013)

smo

protein-protein

Interacting group

Assay

References

smo

pull down, anti tag western blot, fluorescent resonance energy transfer, anti tag coimmunoprecipitation, fluorescence microscopy, anti bait coimmunoprecipitation, western blot, autoradiography, bioluminescence resonance energy transfer, fluorescence technology

(Jiang et al., 2016, Maier et al., 2014, Fan et al., 2012, Chen et al., 2010, Walthall et al., 2007, Zhao et al., 2007)

smo - ci

anti bait coimmunoprecipitation, peptide massfingerprinting, western blot

(Walthall et al., 2007, Lum et al., 2003, Ruel et al., 2003)

smo - cos

bioluminescence resonance energy transfer, fluorescence technology, anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, anti tag western blot, colocalization, fluorescence microscopy, inferred by author, tag visualisation, fluorescent resonance energy transfer, pull down, two hybrid, peptide massfingerprinting

(Li et al., 2014, Maier et al., 2014, Ranieri et al., 2014, Fan et al., 2012, Ranieri et al., 2012, Shi et al., 2011, Zhang et al., 2011, Liu et al., 2007, Ruel et al., 2007, Walthall et al., 2007, Zhang et al., 2005, Jia et al., 2003, Lum et al., 2003, Ogden et al., 2003, Ruel et al., 2003)

smo - Cul4

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018)

smo - flw

anti tag coimmunoprecipitation, western blot

(Su et al., 2011)

smo - fu

anti bait coimmunoprecipitation, western blot, bimolecular fluorescence complementation, fluorescence microscopy, two hybrid, anti tag coimmunoprecipitation, anti tag western blot, colocalization, inferred by author, peptide massfingerprinting

(Kubíková et al., 2023, Giordano et al., 2018, Ranieri et al., 2014, Ranieri et al., 2012, Malpel et al., 2007, Jia et al., 2003, Lum et al., 2003, Ogden et al., 2003, Ruel et al., 2003)

smo - Gβ13F

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018)

smo - gish

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2016)

smo - Gprk2

anti tag coimmunoprecipitation, anti tag western blot, fluorescent resonance energy transfer, fluorescence microscopy, pull down

(Jiang et al., 2016, Chen et al., 2010)

smo - Herc4

anti tag coimmunoprecipitation, anti tag western blot

(Jiang et al., 2019)

smo - Hrs

pull down, anti tag western blot, anti tag coimmunoprecipitation, western blot

(Zhou et al., 2018, Fan et al., 2013)

smo - krz

anti tag coimmunoprecipitation, western blot, anti tag western blot

(Jiang et al., 2018, Zhang et al., 2017, Li et al., 2012, Molnar et al., 2011)

smo - mts

anti tag coimmunoprecipitation, western blot

(Su et al., 2011)

smo - pic

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018)

smo - Pka-C1

anti tag coimmunoprecipitation, anti tag western blot, bimolecular fluorescence complementation, fluorescence microscopy, western blot, anti bait coimmunoprecipitation

(Fan et al., 2019, Chen et al., 2017, Li et al., 2014, Ranieri et al., 2014)

smo - Pp1-13C

anti tag coimmunoprecipitation, western blot

(Su et al., 2011)

smo - Pp1-87B

anti tag coimmunoprecipitation, western blot

(Su et al., 2011)

smo - Pp1α-96A

anti tag coimmunoprecipitation, western blot

(Su et al., 2011)

smo - Pp4-19C

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Jia et al., 2009)

smo - ptc

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation

(Walthall et al., 2007, Johnson et al., 2000)

smo - Rack1

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2020)

smo - smg

colocalization, fluorescence microscopy, inferred by author, anti tag coimmunoprecipitation, anti tag western blot, pull down, molecular weight estimation by staining, isothermal titration calorimetry, predetermined participant, x-ray crystallography, two hybrid

(Kubíková et al., 2023, Bruzzone et al., 2020)

smo - Smurf

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018, Huang et al., 2013)

smo - Sumo

anti tag coimmunoprecipitation, anti tag western blot

(Zhang et al., 2017)

smo - Sxl

anti bait coimmunoprecipitation, western blot

(Walthall et al., 2007)

smo - Ubi-p5E

anti tag coimmunoprecipitation, covalent binding, anti tag western blot

(Yang et al., 2013)

smo - Ubi-p63E

anti tag coimmunoprecipitation, covalent binding, anti tag western blot

(Yang et al., 2013)

smo - Uch-L5

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation

(Zhou et al., 2018)

smo - Uch-L5R

anti tag coimmunoprecipitation, anti tag western blot

(Zhou et al., 2018)

smo - Ulp1

anti tag coimmunoprecipitation, anti tag western blot

(Zhang et al., 2017, Ma et al., 2016)

smo - Usp8

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Li et al., 2020, Ma et al., 2016, Fan et al., 2013, Li et al., 2012, Xia et al., 2012)

smo - Vps36

anti tag coimmunoprecipitation, anti tag western blot

(Yang et al., 2013)

smo - wdb

pull down, western blot, anti tag coimmunoprecipitation

(Su et al., 2011)

RNA-RNA

Interacting group

Assay

References

smo - mir-5

immunohistochemistry, luminiscence technology, necessary binding region, fluorescence technology

(Wu et al., 2012)

smo - mir-12

fluorescence technology

(Friggi-Grelin et al., 2008)

smo - mir-283

fluorescence technology

(Friggi-Grelin et al., 2008)

smo - mir-960

immunocytochemistry, luminiscence technology, necessary binding region, fluorescence technology

(Gao et al., 2013)

nej

protein-protein

Interacting group

Assay

References

nej

enzymatic study, western blot

(Tie et al., 2016)

nej - arm

anti bait coimmunoprecipitation, western blot, pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2007)

nej - asf1

anti bait coimmunoprecipitation, western blot

(Das et al., 2009)

nej - bcd

pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Fu et al., 2004)

nej - brm

pull down, anti tag western blot, western blot, molecular sieving, anti bait coimmunoprecipitation

(Tie et al., 2012)

nej - CG14451

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

nej - ci

two hybrid, anti tag coimmunoprecipitation, anti tag western blot, lambda phage display, pull down, molecular weight estimation by staining

(Han et al., 2019, Han et al., 2015, Chen et al., 2000, Akimaru et al., 1997)

nej - Clk

anti bait coimmunoprecipitation, autoradiography

(Hung et al., 2007)

nej - dl

anti bait coimmunoprecipitation, western blot

(Holmqvist et al., 2012)

nej - EcR

anti tag coimmunoprecipitation, anti tag western blot

(Kirilly et al., 2011)

nej - en

anti tag coimmunoprecipitation, anti tag western blot

(Luo et al., 2017)

nej - gcm

anti bait coimmunoprecipitation, anti tag western blot, electrophoretic mobility shift assay, autoradiography

(Flici et al., 2014, Sorge et al., 2012)

nej - HDAC1

anti bait coimmunoprecipitation, western blot

(Tie et al., 2016)

nej - His3

histone acetylase assay, western blot

(Tie et al., 2012)

nej - HnRNP-K

anti bait coimmunoprecipitation, western blot

(Mallik and Lakhotia, 2010)

nej - Hrb87F

anti bait coimmunoprecipitation, western blot

(Mallik and Lakhotia, 2010)

nej - JIL-1

anti bait coimmunoprecipitation, western blot

(Kellner et al., 2012)

nej - Jra

enzymatic study, western blot

(Zhang et al., 2013)

nej - Lpt

anti tag coimmunoprecipitation, western blot

(Zraly et al., 2020)

nej - Mad

pull down, autoradiography, two hybrid

(Lilja et al., 2007, Waltzer and Bienz, 1999)

nej - Mef2

two hybrid

(Lin and Baines, 2019)

nej - mei-41

anti tag coimmunoprecipitation, anti tag western blot

(Smolik and Jones, 2007)

nej - MEP-1

anti bait coimmunoprecipitation, Identification by mass spectrometry

(Reddy et al., 2010)

nej - Mi-2

anti bait coimmunoprecipitation, Identification by mass spectrometry

(Reddy et al., 2010)

nej - Pc

pull down, western blot, enzymatic study, anti bait coimmunoprecipitation

(Tie et al., 2016)

nej - Polr2A

anti bait coimmunoprecipitation, western blot

(Tie et al., 2016)

nej - Psc

anti bait coimmunoprecipitation, western blot

(Tie et al., 2016)

nej - repo

anti bait coimmunoprecipitation, western blot

(Flici et al., 2014)

nej - Sbf

ion exchange chromatography, molecular sieving, peptide massfingerprinting, western blot

(Petruk et al., 2001)

nej - Sirt1

anti bait coimmunoprecipitation, western blot, pull down, autoradiography

(Smolik, 2009)

nej - TfIIB

anti bait coimmunoprecipitation, anti tag western blot

(Boija et al., 2017)

nej - trr

anti bait coimmunoprecipitation, western blot

(Tie et al., 2014)

nej - trx

pull down, western blot, anti tag western blot, anti bait coimmunoprecipitation, ion exchange chromatography, molecular sieving, peptide massfingerprinting

(Tie et al., 2014, Petruk et al., 2001)

nej - Utx

anti bait coimmunoprecipitation, western blot, pull down, anti tag western blot, molecular sieving

(Tie et al., 2012)

RNA-protein

Interacting group

Assay

References

nej - Fmr1

anti tag coimmunoprecipitation, reverse transcription pcr

(Li and Gavis, 2022)

protein-protein

Interacting group

Assay

References

anti tag coimmunoprecipitation, anti tag western blot, fluorescent resonance energy transfer, fluorescence microscopy

(Zhang et al., 2009)

ci - Ada2b

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

ci - bur

anti tag coimmunoprecipitation, anti tag western blot

(Zhou et al., 2015)

ci - CkIα

anti tag coimmunoprecipitation, anti tag western blot

(Shi et al., 2011, Zhang et al., 2005)

ci - cos

molecular sieving, western blot, anti bait coimmunoprecipitation, peptide massfingerprinting, two hybrid, anti tag coimmunoprecipitation, anti tag western blot, pull down, autoradiography, colocalization, fluorescence microscopy, inferred by author

(Li et al., 2020, Oh et al., 2015, Zhou et al., 2015, Ranieri et al., 2012, Shi et al., 2011, Zhou and Kalderon, 2010, Farzan et al., 2009, Ruel et al., 2007, Zhang et al., 2005, Stegman et al., 2004, Wang and Jiang, 2004, Lum et al., 2003, Ruel et al., 2003, Monnier et al., 2002, Stegman et al., 2000, Wang et al., 2000, Robbins et al., 1997, Sisson et al., 1997)

ci - croc

anti tag coimmunoprecipitation, anti tag western blot

(Tu et al., 2020)

ci - Cul1

pull down, anti tag western blot, enzymatic study, peptide massfingerprinting

(Zhang et al., 2013, Smelkinson et al., 2007)

ci - Cul3

enzymatic study, peptide massfingerprinting

(Zhang et al., 2013)

ci - dbr

anti tag coimmunoprecipitation, autoradiography, anti bait coimmunoprecipitation, anti tag western blot, pull down, two hybrid

(Dai et al., 2003)

ci - dco

enzymatic study, autoradiography, interologs mapping, inferred by curator

(Shi et al., 2014)

ci - eff

enzymatic study, western blot

(Pan et al., 2017)

ci - fu

anti bait coimmunoprecipitation, western blot, enzymatic study, two hybrid, molecular sieving, anti tag coimmunoprecipitation, anti tag western blot, protein three hybrid

(Zhou et al., 2022, Han et al., 2019, Farzan et al., 2009, Paces-Fessy et al., 2004, Stegman et al., 2004, Wang and Jiang, 2004, Horabin et al., 2003, Stegman et al., 2000, Monnier et al., 1998, Robbins et al., 1997)

ci - Gug

anti tag coimmunoprecipitation, anti tag western blot, two hybrid, pull down

(Zhang et al., 2013)

ci - HDAC1

anti tag coimmunoprecipitation, anti tag western blot

(Zhang et al., 2013)

ci - hpo

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2015)

ci - hyd

coimmunoprecipitation, western blot

(Moncrieff et al., 2015)

ci - hyx

pull down, autoradiography

(Mosimann et al., 2009)

ci - Kap-α3

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Seong et al., 2010)

ci - kto

anti tag coimmunoprecipitation, anti tag western blot

(Mao et al., 2014)

ci - lwr

pull down, western blot

(Lv et al., 2016)

ci - mts

anti tag coimmunoprecipitation, western blot

(Su et al., 2011)

ci - nej

two hybrid, anti tag coimmunoprecipitation, anti tag western blot, lambda phage display, pull down, molecular weight estimation by staining

(Han et al., 2019, Han et al., 2015, Chen et al., 2000, Akimaru et al., 1997)

ci - Npl4

pull down, western blot

(Zhang et al., 2013)

ci - Pka-C1

bimolecular fluorescence complementation, fluorescence microscopy, anti tag coimmunoprecipitation, anti tag western blot

(Ranieri et al., 2014)

ci - Polr2C

anti tag coimmunoprecipitation, anti tag western blot

(Gao et al., 2023)

ci - Polr2G

anti tag coimmunoprecipitation, anti tag western blot

(Gao et al., 2023)

ci - ptc

anti bait coimmunoprecipitation, western blot

(Walthall et al., 2007)

ci - Rack1

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2020)

ci - Rbfox1

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot, pull down

(Usha and Shashidhara, 2010)

ci - rdx

pull down, anti tag western blot, western blot, anti tag coimmunoprecipitation, two hybrid, enzymatic study, peptide massfingerprinting

(Han et al., 2019, Zhou et al., 2015, Liu et al., 2014, Shi et al., 2014, Zhang et al., 2013, Seong et al., 2010, Zhang et al., 2009, Kent et al., 2006, Zhang et al., 2006)

ci - sgg

anti tag coimmunoprecipitation, anti tag western blot

(Shi et al., 2011, Zhang et al., 2005)

ci - skd

anti tag coimmunoprecipitation, anti tag western blot

(Mao et al., 2014)

ci - SkpA

pull down, anti tag western blot

(Smelkinson et al., 2007)

ci - slmb

pull down, autoradiography, enzymatic study, peptide massfingerprinting, anti tag western blot

(Zhang et al., 2013, Smelkinson et al., 2007, Smelkinson and Kalderon, 2006, Jia et al., 2005, Dai et al., 2003)

ci - smo

anti bait coimmunoprecipitation, peptide massfingerprinting, western blot

(Walthall et al., 2007, Lum et al., 2003, Ruel et al., 2003)

ci - sqh

anti tag coimmunoprecipitation, anti tag western blot, pull down, Identification by mass spectrometry

(Liu et al., 2015)

ci - Stat92E

anti tag coimmunoprecipitation, anti tag western blot

(Liu et al., 2015)

ci - Su(fu)

pull down, western blot, anti tag coimmunoprecipitation, anti tag western blot, molecular sieving, anti bait coimmunoprecipitation, autoradiography, fluorescent resonance energy transfer, fluorescence microscopy, two hybrid, isothermal titration calorimetry, predetermined participant

(Zhou et al., 2022, Han et al., 2019, Han et al., 2015, Oh et al., 2015, Zhou et al., 2015, Shi et al., 2014, Zhang et al., 2013, Shi et al., 2011, Farzan et al., 2009, Zhang et al., 2006, Paces-Fessy et al., 2004, Wang and Jiang, 2004, Lum et al., 2003, Methot and Basler, 2000, Stegman et al., 2000, Monnier et al., 1998, Paumard-Rigal et al., 1998)

ci - Sumo

pull down, western blot

(Lv et al., 2016)

ci - Sxl

anti bait coimmunoprecipitation, western blot

(Walthall et al., 2007, Horabin et al., 2003)

ci - tai

electrophoretic mobility shift assay, anti tag coimmunoprecipitation, anti tag western blot

(Yu et al., 2024)

ci - Tnpo

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Han et al., 2019, Shi et al., 2014)

ci - tws

anti tag coimmunoprecipitation, western blot

(Su et al., 2011)

ci - Usp7

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Sun et al., 2019, Zhou et al., 2015)

ci - yki

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2015)

Sxl

protein-protein

Interacting group

Assay

References

Sxl

anti bait coimmunoprecipitation, quantitative reverse transcription pcr, reverse transcription pcr, anti tag coimmunoprecipitation, primer specific pcr, western blot, cross-linking study, full identification by RNA sequencing

(Fan et al., 2023, Sandler et al., 2018, Kan et al., 2017, Haussmann et al., 2016, Yan and Perrimon, 2015, Graham et al., 2011, Johnson et al., 2010, Walthall et al., 2007)

Sxl - bam

molecular sieving, western blot

(Li et al., 2013)

Sxl - bgcn

anti tag coimmunoprecipitation, anti tag western blot, molecular sieving, western blot

(Malik et al., 2017, Li et al., 2013)

Sxl - brat

anti tag coimmunoprecipitation, anti tag western blot, two hybrid, bimolecular fluorescence complementation, fluorescence microscopy

(Malik et al., 2017)

Sxl - ci

anti bait coimmunoprecipitation, western blot

(Walthall et al., 2007, Horabin et al., 2003)

Sxl - cos

anti bait coimmunoprecipitation, western blot

(Walthall et al., 2007, Horabin et al., 2003)

Sxl - eIF4E1

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Graham et al., 2011)

Sxl - fl(2)d

anti bait coimmunoprecipitation, western blot

(Penn et al., 2008)

Sxl - fu

anti bait coimmunoprecipitation, western blot

(Horabin et al., 2003)

Sxl - how

pull down, peptide massfingerprinting, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot

(Graindorge et al., 2013)

Sxl - Hrb27C

anti bait coimmunoprecipitation, western blot

(Szostak et al., 2018)

Sxl - loqs

pull down, peptide massfingerprinting

(Graindorge et al., 2013)

Sxl - me31B

pull down, peptide massfingerprinting

(Graindorge et al., 2013)

Sxl - mei-P26

molecular sieving, western blot, two hybrid, anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation

(Malik et al., 2017, Li et al., 2013)

Sxl - nito

anti tag coimmunoprecipitation, anti tag western blot, primer specific pcr

(Yan and Perrimon, 2015)

Sxl - Polr3E

pull down, autoradiography, two hybrid

(Dong and Bell, 1999)

Sxl - pps

anti bait coimmunoprecipitation, western blot, reverse transcription pcr

(Johnson et al., 2010)

Sxl - ptc

anti bait coimmunoprecipitation, western blot

(Walthall et al., 2007)

Sxl - smo

anti bait coimmunoprecipitation, western blot

(Walthall et al., 2007)

Sxl - snf

pull down, western blot, anti bait coimmunoprecipitation, electrophoretic mobility shift assay, autoradiography, reverse transcription pcr

(Johnson et al., 2010, Hu et al., 2009, Kalifa et al., 2009, Penn et al., 2008)

Sxl - Su(fu)

anti bait coimmunoprecipitation, western blot

(Horabin et al., 2003)

Sxl - tral

pull down, peptide massfingerprinting

(Graindorge et al., 2013)

Sxl - U2af50

anti bait coimmunoprecipitation, western blot

(Penn et al., 2008)

Sxl - Unr

pull down, western blot, anti bait coimmunoprecipitation, autoradiography, electrophoretic mobility shift assay, affinity chromatography technology, Identification by mass spectrometry

(Graindorge et al., 2013, Abaza and Gebauer, 2008, Abaza et al., 2006)

RNA-protein

Interacting group

Assay

References

Sxl

(Fan et al., 2023, Sandler et al., 2018, Kan et al., 2017, Haussmann et al., 2016, Yan and Perrimon, 2015, Graham et al., 2011, Johnson et al., 2010, Walthall et al., 2007)

Sxl - aub

iclip, partial RNA sequence identification

(Ma et al., 2017)

Sxl - bru1

electrophoretic mobility shift assay, autoradiography

(Wang and Lin, 2007)

Sxl - CG5050

enzymatic study, inferred by author

(Medenbach et al., 2011)

Sxl - CG31908

enzymatic study, inferred by author

(Medenbach et al., 2011)

Sxl - eIF4E1

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Graham et al., 2011)

Sxl - Gs2

electrophoretic mobility shift assay, autoradiography

(Banerjee and Singh, 2021)

Sxl - hoe1

enzymatic study, inferred by author

(Medenbach et al., 2011)

Sxl - Lrr47

enzymatic study, inferred by author

(Medenbach et al., 2011)

Sxl - msl-2

enzymatic study, inferred by author, x-ray crystallography, nucleic acid uv cross-linking assay, autoradiography, electrophoretic mobility shift assay, static light scattering, affinity chromatography technology, western blot, anti bait coimmunoprecipitation, quantitative reverse transcription pcr, cross-linking study, full identification by RNA sequencing, isothermal titration calorimetry, predetermined participant, reverse transcription pcr, pull down

(Fan et al., 2023, Moschall et al., 2018, Sandler et al., 2018, Szostak et al., 2018, Hennig et al., 2014, Graindorge et al., 2013, Medenbach et al., 2011, Abaza and Gebauer, 2008, Abaza et al., 2006, Grskovic et al., 2003, Kelley et al., 1997)

Sxl - N

nucleic acid uv cross-linking assay, autoradiography, anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Penn and Schedl, 2007)

Sxl - Nab2

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Jalloh et al., 2023)

Sxl - nanos

anti bait coimmunoprecipitation, quantitative reverse transcription pcr, anti tag coimmunoprecipitation, clip, primer specific pcr

(Malik et al., 2020, Morita et al., 2018, Li et al., 2013, Chau et al., 2012)

Sxl - NetA

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Sandler et al., 2018)

Sxl - NHP2

clip, quantitative reverse transcription pcr

(Morita et al., 2018)

Sxl - nito

anti tag coimmunoprecipitation, anti tag western blot, primer specific pcr

(Yan and Perrimon, 2015)

Sxl - Pka-C3

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Sandler et al., 2018)

Sxl - pps

anti bait coimmunoprecipitation, western blot, reverse transcription pcr

(Johnson et al., 2010)

Sxl - RpS14a

enzymatic study, inferred by author

(Medenbach et al., 2011)

Sxl - sca

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Sandler et al., 2018)

Sxl - S-Lap3

enzymatic study, inferred by author

(Medenbach et al., 2011)

Sxl - snf

pull down, western blot, anti bait coimmunoprecipitation, electrophoretic mobility shift assay, autoradiography, reverse transcription pcr

(Johnson et al., 2010, Hu et al., 2009, Kalifa et al., 2009, Penn et al., 2008)

Sxl - sog

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Sandler et al., 2018)

Sxl - ssx

clip-seq

(Moschall et al., 2019)

Sxl - tra

electrophoretic mobility shift assay, autoradiography, nucleic acid uv cross-linking assay, molecular weight estimation by staining, anti bait coimmunoprecipitation, reverse transcription pcr, quantitative reverse transcription pcr

(Banerjee and Singh, 2021, Sandler et al., 2018, Johnson et al., 2010, Valcarcel et al., 1993)

Sxl - wupA

electrophoretic mobility shift assay, autoradiography

(Banerjee and Singh, 2021)

Sxl - Ythdc1

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Kan et al., 2017, Haussmann et al., 2016)

Sxl - Ythdf

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Kan et al., 2017)

Su(fu)

protein-protein

Interacting group

Assay

References

Su(fu) - ci

Su(fu) - cos

anti bait coimmunoprecipitation, western blot, pull down, molecular sieving

(Farzan et al., 2009, Lum et al., 2003, Stegman et al., 2000)

Su(fu) - fu

x-ray crystallography, isothermal titration calorimetry, predetermined participant, two hybrid, anti bait coimmunoprecipitation, western blot, molecular sieving, molecular weight estimation by staining, pull down, autoradiography, protein kinase assay, anti tag western blot, anti tag coimmunoprecipitation, affinity chromatography technology

(Li et al., 2020, Oh et al., 2015, Zhang et al., 2011, Farzan et al., 2009, Paces-Fessy et al., 2004, Lum et al., 2003, Stegman et al., 2000, Monnier et al., 1998)

Su(fu) - Sxl

anti bait coimmunoprecipitation, western blot

(Horabin et al., 2003)

Alleles Reported to Model Human Disease (Disease Ontology) (32 alleles)

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

hh^RNAi.UAS.cUa

model of primary bacterial infectious disease

CEA

(Lee et al., 2015)

Modifiers Based on Experimental Evidence ( 4 )

Allele

Disease

Interaction

References

hh²

exacerbates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

exacerbates multiple endocrine neoplasia type 2A

modeled by Ret^MEN2A.GMR

(Read et al., 2005)

hh^AC

exacerbates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

hh^neo56

ameliorates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

hh^rJ413

exacerbates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

exacerbates multiple endocrine neoplasia type 2A

modeled by Ret^MEN2A.GMR

(Read et al., 2005)

smo

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

smo^RNAi.UAS.cUa

model of primary bacterial infectious disease

CEA

(Lee et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

smo¹

ameliorates neurodegenerative disease

modeled by ptc^559.1

(Gazi et al., 2009)

nej

Models Based on Experimental Evidence ( 4 )

Allele

Disease

Evidence

References

nej^Q7

model of alcohol use disorder

CEA

(Ghezzi et al., 2017)

nej³

model of alcohol use disorder

CEA

(Ghezzi et al., 2017)

nej^hs.PA

model of alcohol use disorder

CEA

(Ghezzi et al., 2017)

nej^P

model of Rubinstein-Taybi syndrome

CEA

(Di Fede et al., 2021)

Modifiers Based on Experimental Evidence ( 20 )

Allele

Disease

Interaction

References

nej^Q7

ameliorates Schimke immuno-osseous dysplasia

modeled by Marcal1^UAS.cBa

(Morimoto et al., 2016)

nej³

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Bodai et al., 2012)

exacerbates acute myeloid leukemia

modeled by Hsap\HOXA9::Hsap\NUP98^WT.UAS

(Gavory et al., 2021)

nej^UAS.Tag:V5

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q27.UAS.Tag:HA}

(Cho et al., 2022)

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

ameliorates acute myeloid leukemia

modeled by Hsap\HOXA9::Hsap\NUP98^WT.UAS

(Gavory et al., 2021)

nej^KIX.UAS

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

nej^RNAi.UAS.cKa

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Raj and Sarkar, 2019)

nej^{F2161A.UAS.Tag:V5}

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q27.UAS.Tag:HA}

(Cho et al., 2022)

nej^EP950

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN7^{102Q.T.UAS.Tag:NLS(SV40-largeT)}

(Latouche et al., 2007)

nej^ΔBHQ.UAS

exacerbates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Raj and Sarkar, 2019)

nej^ΔHQ.UAS

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

nej^hs.PA

model of alcohol use disorder

is ameliorated by slo^MI02233

(Ghezzi et al., 2017)

nej^ΔNZK.UAS

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

nej^ΔQ.UAS

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

nej^P

exacerbates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Taylor et al., 2003)

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Jung and Bonini, 2007)

modeled by Hsap\ATXN3^tr.Q46.UAS

(Jung and Bonini, 2007)

nej^EP1410

ameliorates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Huen and Chan, 2005)

nej^GD8975

ameliorates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Martin et al., 2021)

nej^KK113191

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q27.UAS.Tag:HA}

(Cho et al., 2022)

ameliorates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Martin et al., 2021)

nej^HMS01507

ameliorates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Martin et al., 2021)

nej^EP1149

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Taylor et al., 2003)

nej^EP1179

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Taylor et al., 2003)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Jung and Bonini, 2007)

modeled by Hsap\ATXN3^tr.Q46.UAS

(Jung and Bonini, 2007)

nej^RNAi.UAS.cSa

ameliorates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Martin et al., 2021)

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

ci^{-3P.UAS.W.Tag:HA}

model of cancer

CEA with rdx^NIG.9924R

(Gao et al., 2023)

CEA with Mmus\Gli2^UAS.Tag:MYC

(Gao et al., 2023)

ci^RNAi.UAS.cUa

model of primary bacterial infectious disease

CEA

(Lee et al., 2015)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

ci^5m.UAS

exacerbates cancer

modeled by Hipk^UAS.Tag:HA

(Blaquiere et al., 2018)

ci^76.UAS

DOES NOT ameliorate cancer

modeled by Hipk^UAS.Tag:HA

(Blaquiere et al., 2018)

ci^RNAi.UAS.cUa

ameliorates atypical teratoid rhabdoid tumor

modeled by Snr1^RNAi.UAS.cUa

(Jeibmann et al., 2014)

Sxl

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Sxl^cF1.otu

ameliorates germ cell cancer

modeled by snf¹⁴⁸

(Chau et al., 2009)

Alleles Representing Disease-Implicated Variants

Genetic Tools, Stocks and Reagents

Sources of Stocks

Contact lab of origin for a reagent not available from a public stock center.

Bloomington Stock Center Disease Page

Related mammalian, viral, bacterial, or synthetic transgenes

Allele

Transgene

Publicly Available Stocks

Selected Drosophila transgenes

Allele

Transgene

Publicly Available Stocks

ci^{-3P.UAS.W.Tag:HA}

w^*; P{UAS-nej.wt-V5}3

nej^KIX.UAS

P{UAS-nej.KIX}

nej^{F2161A.UAS.Tag:V5}

P{UAS-nej.F2161A-V5}

w^*; P{UAS-nej.F2161A-V5}2

y¹ w¹ snf¹ sn³; P{otu-Sxl.cF1}A

RNAi constructs available

Allele

Transgene

Publicly Available Stocks

smo^{RNAi.3'UTR.UAS}

P{UAS-smo.RNAi.3'UTR}

y¹ w^*; P{UAS-smo.RNAi.WIZ}2 P{UAS-smo.RNAi.WIZ}8/CyO, P{Wee-P.ph0}2

y¹ v¹; P{TRiP.JF02363}attP2

y¹ v¹; P{TRiP.GL01472}attP2

smo^HMC03577

P{TRiP.HMC03577}

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03577}attP40

y¹ v¹; P{TRiP.HMJ30064}attP40/CyO

w¹¹¹⁸; P{GD14317}v29897

y¹ v¹; P{TRiP.HMJ21803}attP40/CyO

ihog^HMC05560

P{TRiP.HMC05560}

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05560}attP40

w¹¹¹⁸ P{GD11802}v35055

Su(fu)^HM05045

P{TRiP.HM05045}

y¹ v¹; P{TRiP.HM05045}attP2

y¹ v¹; P{TRiP.HMC04002}attP40

y¹ v¹; P{TRiP.HMJ23860}attP40/CyO

w¹¹¹⁸; P{GD1403}v51479

y¹ v¹; P{TRiP.JF01715}attP2

ci^JF01271

P{TRiP.JF01271}

y¹ v¹; P{TRiP.JF01271}attP2

ci^JF01272

P{TRiP.JF01272}

y¹ v¹; P{TRiP.JF01272}attP2

ci^JF01744

P{TRiP.JF01744}

y¹ v¹; P{TRiP.JF01744}attP2

P{UAS-hh.TH201500473.S}

w¹¹¹⁸; P{GD6242}v43255

hh^JF01804

P{TRiP.JF01804}

y¹ v¹; P{TRiP.JF01804}attP2

y¹ v¹; P{TRiP.JF01270}attP2

hh^JF01488

P{TRiP.JF01488}

y¹ v¹; P{TRiP.JF01488}attP2

hh^HMS00492

P{TRiP.HMS00492}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00492}attP2/TM3, Sb¹

w¹¹¹⁸; P{GD8975}v46534

y¹ v¹; P{TRiP.JF02806}attP2

nej^HM04037

P{TRiP.HM04037}

y¹ v¹; P{TRiP.HM04037}attP2

nej^RNAi.UAS.cSa

P{UAS-nej.RNAi.S}

P{UAS-nej.RNAi.S}hp12, w^*

nej^HMS01507

P{TRiP.HMS01507}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01507}attP2

nej^HMS01570

P{TRiP.HMS01570}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01570}attP2/TM3, Sb¹

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00609}attP2

y¹ sc^* v¹ sev²¹; P{TRiP.GL00634}attP40

Selected Drosophila classical alleles

Allele

Allele class

Mutagen

Publicly Available Stocks

smo^D16

loss of function allele

gamma ray

w^*; smo^D16 P{neoFRT}42D mam¹⁰ Egfr^f24/CyO, P{ActGFP}JMR1

smo¹

ethyl methanesulfonate

CyO / smo¹ cn¹ bw¹ speck¹

ihog^DC1

amorphic allele - molecular evidence

FLPase

ihog^GLC

amorphic allele - molecular evidence

ends-out gene targeting

Su(fu)^LP

amorphic allele - genetic evidence

ethyl methanesulfonate

w^*; P{neoFRT}82B Df(3R)Su(fu)^LP, Su(fu)^LP, P{PZ}Gprk2⁰⁶⁹³⁶ PNPase⁰⁶⁹³⁶/TM6B, Tb¹

ci^DR50

loss of function allele

gamma ray

ci^D

loss of function allele

X ray

ci^D / l(4)102ABd⁴

ci⁹⁴

loss of function allele

Delta2-3 transposase

P{hsFLP}12, y¹ w^*; P{ci^+tWa}2L P{neoFRT}40A; ci⁹⁴

hh^ΔaptDB1

loss of function allele

CRISPR/Cas9

hh^ΔaptDB2

loss of function allele

CRISPR/Cas9

hh^ΔaptDB3

loss of function allele

CRISPR/Cas9

hh^ΔaptDB4

loss of function allele

CRISPR/Cas9

hh^ΔaptDB5

loss of function allele

CRISPR/Cas9

hh^ΔaptDB6

loss of function allele

CRISPR/Cas9

hh^rJ413

P-element activity

ry⁵⁰⁶ P{PZ}hh^rJ413 CG31457^rJ413/TM3, ry^RK Sb¹ Ser¹

hh^neo56

P-element activity

hh^A

loss of function allele

Delta2-3 transposase

hh^AC

amorphic allele - genetic evidence

Delta2-3 transposase

ry⁵⁰⁶ hh^AC/TM3, Sb¹

hh^AE

amorphic allele - genetic evidence

Delta2-3 transposase

hh^AM

loss of function allele

Delta2-3 transposase

hh¹¹

amorphic allele - genetic evidence

gamma ray

hh²

ethyl methanesulfonate

st¹ e¹ hh²/TM3, Sb¹

hh¹⁰

amorphic allele - genetic evidence

gamma ray

hh³

loss of function allele

ethyl methanesulfonate

hh⁸

amorphic allele - genetic evidence

ethyl methanesulfonate

hh²¹

loss of function allele

ethyl methanesulfonate

P{ftz-lacC}4; hh²¹/TM3, Sb¹

nej^P

P-element activity

w^* P{lacW}nej^P/FM7c

nej^EP950

P-element activity

w¹¹¹⁸ P{EP}nej^EP950 EP950^EP950

P-element activity

P-element activity

ethyl methanesulfonate

y¹ nej^Q7 v¹ f¹/Dp(1;Y)FF1, y⁺/C(1)DX, y¹ w¹ f¹

P-element activity

P-element activity

loss of function allele

P-element activity

Sxl^fPED1

loss of function allele

PM hybrid dysgenesis

Sxl^fPED2

loss of function allele

PM hybrid dysgenesis

Sxl^M1,fPa

amorphic allele - genetic evidence

PM hybrid dysgenesis

Sxl^f2

loss of function allele

spontaneous

y¹ Sxl^f2/FM7c

Sxl^fLS

loss of function allele

spontaneous

Sxl^fPb

loss of function allele

PM hybrid dysgenesis

Sxl^fhv1

loss of function allele

spontaneous

Sxl^M3:d1

amorphic allele - genetic evidence

Sxl^f2593

loss of function allele

ethyl methanesulfonate

w¹ Sxl^f2593 ct⁶; P{Sxl.alt5-C8}2/+

Sxl^f6

loss of function allele

ethyl methanesulfonate

Sxl^f37

loss of function allele

ethyl methanesulfonate

Sxl^fP7B0

loss of function allele

PM hybrid dysgenesis

y¹ pn¹ w¹ Sxl^M8/Df(1)Sxl^fP7B0, y¹ pn¹ w¹ Sxl^fP7B0

Sxl^f7,M1

loss of function allele

gamma ray

w¹ cm¹ Sxl^f7,M1 ct⁶ v¹; P{Sxl.+tCa}9A/+

Sxl^f58

loss of function allele

ethyl methanesulfonate

Sxl^fP3G2

loss of function allele

PM hybrid dysgenesis

Sxl^M1,f3

loss of function allele

gamma ray

Sxl^f9

loss of function allele

ethyl methanesulfonate

w¹ Sxl^f9 ct⁶/Binsinscy, l(1)*^*

Sxl^fP6C2

loss of function allele

PM hybrid dysgenesis

Sxl^f10,M1

amorphic allele - genetic evidence

gamma ray

Sxl^f1

loss of function allele

spontaneous

y¹ cm¹ Sxl^f1 v¹ f¹/FM7a/Dp(1;2;Y)w⁺

References (6)

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