FB2026_02 , released June 18, 2026
Gene: Dmel\Rbf
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General Information
Symbol
Dmel\Rbf
Species
D. melanogaster
Name
Retinoblastoma-family protein
Annotation Symbol
CG7413
Feature Type
protein_coding_gene
FlyBase ID
FBgn0015799
Gene Model Status
Current
Stock Availability
32 publicly available
Gene Summary
Retinoblastoma-family protein (Rbf) encodes a protein that binds to members of the E2F transcription factors and forms the DREAM complex, which regulates genes with diverse biological functions including cell cycle regulators, apoptosis regulators, and DNA replication/repair factors. The product of Rbf can also bind chromosomal proteins and modulate genome stability. It is negatively regulated by CycE-Cdk2 and CycD-Cdk4 cyclin-dependent kinase complexes. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Rbf1, Rb, EG:34F3.3 , dRBF, pRb

Key Links
Genomic Location
Cytogenetic map
1C5-1C5
Sequence location
Recombination map
1-0
RefSeq locus
NC_004354 REGION:797090..801479
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (27 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
enables DNA-binding transcription factor binding
inferred from physical interaction with FLYBASE:Dp; FB:FBgn0011763
(Du et al., 1996)
inferred from physical interaction with FLYBASE:E2f1; FB:FBgn0011766
(Du et al., 1996)
enables protein binding
inferred from physical interaction with FLYBASE:Snr1; FB:FBgn0011715
(Brumby et al., 2002)
inferred from physical interaction with UniProtKB:O77051
(Frolov et al., 2001)
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding
inferred from mutant phenotype
(Kim et al., 2018)
inferred from direct assay
(Lee et al., 2012)
enables transcription cis-regulatory region binding
inferred from direct assay
(Ullah et al., 2007)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000348425
(GO Reference Genome Project, 2011-)
Biological Process (18 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
involved_in chromosome organization
inferred from mutant phenotype
(Manning et al., 2010)
involved_in DNA endoreduplication
inferred from mutant phenotype
(Shcherbata et al., 2004)
involved_in intrinsic apoptotic signaling pathway in response to DNA damage
inferred from mutant phenotype
(Moon et al., 2008)
involved_in maintenance of mitotic sister chromatid cohesion
inferred from mutant phenotype
(Manning et al., 2010)
involved_in negative regulation of apoptotic process
inferred from mutant phenotype
(Tanaka-Matakatsu et al., 2009)
involved_in negative regulation of DNA replication
inferred from mutant phenotype
(Bosco et al., 2001)
involved_in negative regulation of G1/S transition of mitotic cell cycle
inferred from mutant phenotype
(Firth and Baker, 2005, Du and Dyson, 1999)
involved_in negative regulation of gene expression
inferred from mutant phenotype
(Lu et al., 2007)
involved_in negative regulation of transcription by RNA polymerase II
inferred from direct assay
(Stevaux et al., 2002, Du et al., 1996)
inferred from mutant phenotype
(Korenjak et al., 2004, Du and Dyson, 1999)
inferred from genetic interaction with FLYBASE:Rbf2; FB:FBgn0038390
(Lewis et al., 2004)
involved_in regulation of cell population proliferation
inferred from mutant phenotype
(Hsieh et al., 2010)
involved_in regulation of cell size
inferred from mutant phenotype
(Elenbaas et al., 2015)
involved_in regulation of chromosome segregation
inferred from mutant phenotype
(Manning et al., 2010)
involved_in regulation of execution phase of apoptosis
inferred from mutant phenotype
(Bhaskar et al., 2014, Zhang et al., 2014)
involved_in response to starvation
inferred from mutant phenotype
(Zhang et al., 2014)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in eggshell chorion gene amplification
traceable author statement
(Lee and Orr-Weaver, 2003, McNairn and Gilbert, 2003)
involved_in endomitotic cell cycle
traceable author statement
(Lee and Orr-Weaver, 2003)
involved_in negative regulation of G1/S transition of mitotic cell cycle
inferred from biological aspect of ancestor with PANTHER:PTN000348425
(GO Reference Genome Project, 2011-)
involved_in regulation of cell cycle
inferred from electronic annotation with InterPro:IPR002719, InterPro:IPR002720, InterPro:IPR028309
(InterPro Project Members, 2004-)
involved_in regulation of transcription by RNA polymerase II
inferred from electronic annotation with InterPro:IPR028309
(InterPro Project Members, 2004-)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
located_in chromatin
inferred from direct assay
(Korenjak et al., 2004)
part_of DRM complex
inferred from direct assay
(Beall et al., 2007)
part_of Myb complex
inferred from direct assay
(Korenjak et al., 2004, Lewis et al., 2004)
located_in nucleus
inferred from direct assay
(Longworth et al., 2012, Acharya et al., 2010)
part_of Rb-E2F complex
inferred from direct assay
(Stevaux et al., 2002, Du et al., 1996)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in chromatin
inferred from biological aspect of ancestor with PANTHER:PTN000348425
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred from electronic annotation with InterPro:IPR002719, InterPro:IPR002720
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the retinoblastoma protein (RB) family. (Q24472)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Retinoblastoma-family protein (Rbf) encodes a protein that binds to members of the E2F transcription factors and forms the DREAM complex, which regulates genes with diverse biological functions including cell cycle regulators, apoptosis regulators, and DNA replication/repair factors. The product of Rbf can also bind chromosomal proteins and modulate genome stability. It is negatively regulated by CycE-Cdk2 and CycD-Cdk4 cyclin-dependent kinase complexes. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
DREAM TRANSCRIPTIONAL REPRESSOR COMPLEX, RBF VARIANT -
The DREAM complex is a MuvB-type transcriptional repressor complex containing multiple site-specific DNA-binding proteins, including E2f2 and Rbf. (Adapted from PMID:35468940 and FBrf0258562.)
Protein Function (UniProtKB)
Functions in cell cycle regulation. Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In follicle cells, the complex plays a central role in the site-specific DNA replication at the chorion loci. During development, the complex represses transcription of developmentally controlled E2F target genes.
(UniProt, Q24472)
Summary (Interactive Fly)

cell cycle regulator - interacts with and negatively regulates members of a family of factors called E2Fs, which serve to activate transcription of genes required for entry into S phase - forms the DREAM complex, which regulates cell cycle regulators, apoptosis regulators, and DNA replication/repair factors - binds chromosomal proteins and modulates genome stability - negatively regulated by CycE-Cdk2 and CycD-Cdk4 cyclin-dependent kinase complexes

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Rbf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q24472)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Rbf-RA
FBtr0070146
NM_080297
3619
845
Additional Transcript Data and Comments
Reported size (kB)

2.593 (unknown)

(Dougan and DiNardo, 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Rbf-PA
FBpp0070141
96.8
845
6.54
Q24472
NP_525036
AAF45551
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

797 (aa); 95 (kD observed)

(Dougan and DiNardo, 1992)
Comments
External Data
Subunit Structure (UniProtKB)

Forms a complex with the DRTF1/E2F transcription factor through binding to a C-terminal region of E2F. This binding inhibits the E2F-mediated transactivation activity. Component of the DREAM complex at least composed of Myb, Caf1-55, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, HDAC1/Rpd3 and l(3)mbt.

(UniProt, Q24472)
Post Translational Modification

Phosphorylation by cyclin E-dependent kinases appears to negatively regulate RBF activity.

(UniProt, Q24472)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rbf using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.56

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
primordial germ cell
(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
dorsal pharyngeal muscle primordium
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
Malpighian tubule primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
dorsal pharyngeal muscle cell
(Fisher et al., 2012)
embryonic anal pad
(Fisher et al., 2012)
embryonic brain
(Fisher et al., 2012)
embryonic foregut
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
embryonic/larval midgut interstitial cell
(Fisher et al., 2012)
larval muscle system
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
larval visceral muscle cell
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
eye disc | ubiquitous
(Moon et al., 2006)
testis | restricted
(Dominado et al., 2016)
cyst cell of testis
(Dominado et al., 2016)
spermatocyte
(Dominado et al., 2016)
pupal stage P5
myoblast
(Zappia et al., 2019)
pharate adult stage P8 -- P15
dorsal longitudinal indirect flight muscle cell
(Zappia et al., 2019)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data

Rbf is highly expressed in proliferating myoblasts at early stage of pupal development as myoblasts are migrating to fuse with forming myotubes. At 14hr APF, the level of Rbf is strikingly lower in myotubes compared to the surrounding pool of migrating myoblasts. Levels remain low in myotubes until fusion is complete and no myoblasts are detectable at 33hr APF. Later in pupal development, Rbf is detected in dissected DLMs at 52hr APF and remains at 80 and 96hr APF. It appears that actively proliferating myoblasts express high levels of Rbf, whereas the expression of Rbf is dramatically reduced as myoblasts undergo fusion to form myotubes.

(Zappia et al., 2019)

Rbf protein is detected in both the germline and somatic lineages in the third instar larval testis. It is particularly abundant in somatic cells. It is normally most abundant in cyst cells and spermatocytes.

(Dominado et al., 2016)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in chromatin
inferred from direct assay
(Korenjak et al., 2004)
part_of DRM complex
inferred from direct assay
(Beall et al., 2007)
part_of Myb complex
inferred from direct assay
(Korenjak et al., 2004, Lewis et al., 2004)
located_in nucleus
inferred from direct assay
(Longworth et al., 2012, Acharya et al., 2010)
part_of Rb-E2F complex
inferred from direct assay
(Stevaux et al., 2002, Du et al., 1996)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Rbf in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 27 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 59 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rbf
Transgenic constructs containing regulatory region of Rbf
Aberrations (Deficiencies and Duplications) ( 7 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal, with Scer\GAL4ap-md544
lethal, with Scer\GAL4da.PU
lethal, with Scer\GAL4ey.PH
lethal | germline clone | rescuable maternal effect
lethal | recessive
lethal - all die before end of pupal stage
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
partially lethal, with Scer\GAL4ey.PH
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4da.PU
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable (with Rbf11)
viable (with Rbf120a)
Sterility
Allele
fertile
sterile (with Rbf11)
sterile (with Rbf120a)
Other Phenotypes
Allele
abnormal cell cycle | adult stage | male, with Scer\GAL4Acp26Aa.PC
abnormal cell cycle | adult stage | male, with Scer\GAL4esg.PU
abnormal cell cycle | embryonic stage
abnormal cell growth | pupal stage | somatic clone
abnormal cell number | somatic clone | spermatogenesis
abnormal cell number | somatic clone | spermatogenesis, with Scer\GAL4Tub.PU
abnormal cell number | spermatogenesis, with Scer\GAL4C587
abnormal cell number | spermatogenesis, with Scer\GAL4NP1624
abnormal developmental rate, with Scer\GAL4hs.PH
abnormal immune response, with Scer\GAL4yolk
abnormal mitotic cell cycle | somatic clone, with Scer\GAL4Act5C.PP
abnormal neuroanatomy, with Scer\GAL4sca-537.4
abnormal size, with Scer\GAL4NP6333
abnormal size | adult stage, with Scer\GAL4ey.PH
abnormal size | adult stage, with Scer\GAL4hs.2sev
abnormal size | third instar larval stage, with Scer\GAL4ey.PH
abnormal starvation stress response
decreased cell number, with Scer\GAL4sca-537.4
decreased cell number | adult stage
decreased cell number | spermatogenesis, with Scer\GAL4NP1624
decreased occurrence of cell division | cell autonomous | larval stage | somatic clone, with Scer\GAL4Act5C.PP
decreased occurrence of cell division | larval stage
decreased occurrence of cell division | larval stage, with Scer\GAL4en-e16E
decreased occurrence of cell division | somatic clone, with Scer\GAL4Act5C.PP
increased cell death
increased cell death, with Scer\GAL4bbg-C96
increased cell death, with Scer\GAL4en.PU
increased cell death, with Scer\GAL4ptc.PU
increased cell death, with Scer\GAL4vg.PM
increased cell death (with Rbf15aΔ)
increased cell death (with Rbf120-3-3)
increased cell death | embryonic stage | maternal effect
increased cell death | larval stage | somatic clone
increased cell death | larval stage | somatic clone, with Scer\GAL4Act.PU
increased cell death | recessive
increased cell death | recessive | somatic clone
increased cell death | somatic clone
increased cell death | somatic clone, with Scer\GAL4unspecified
increased cell death | somatic clone | third instar larval stage
increased cell death | somatic clone | wandering third instar larval stage
increased cell death | third instar larval stage, with Scer\GAL4ap-md544
increased cell death | third instar larval stage, with Scer\GAL4NP6333
increased cell growth rate | adult stage | male, with Scer\GAL4esg.PU
increased cell number | somatic clone | third instar larval stage
increased cell number | spermatogenesis, with Scer\GAL4tj.PU
increased cell size, with Scer\GAL4NP6333
increased occurrence of cell division | adult stage, with Scer\GAL4esg.PU
increased occurrence of cell division | cell non-autonomous | larval stage, with Scer\GAL4en.PU
increased occurrence of cell division | larval stage, with Scer\GAL4en.PU
increased occurrence of cell division | larval stage (with Rbf11)
increased occurrence of cell division | larval stage (with Rbf120a)
increased occurrence of cell division | larval stage | somatic clone
increased occurrence of cell division | oogenesis
increased occurrence of cell division | somatic clone | third instar larval stage
increased occurrence of cell division | spermatogenesis, with Scer\GAL4NP1624
some die during pupal stage
some die during second instar larval stage
some die during third instar larval stage
visible
visible, with Scer\GAL4bbg-C96
visible, with Scer\GAL4Bx-MS1096
visible, with Scer\GAL4da.PU
visible, with Scer\GAL4en-e16E
visible, with Scer\GAL4ey.PH
visible, with Scer\GAL4GMR.PF
visible, with Scer\GAL4hs.PB
visible, with Scer\GAL4NP6333
visible, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4vg.PM
visible | adult stage | somatic clone, with Scer\GAL4Act.PU
wild-type
Phenotype manifest in
Allele
abdomen, with Scer\GAL4hs.PH
adult posterior midgut epithelium, with Scer\GAL4esg.PU
anterior crossvein, with Scer\GAL4dpp.blk1
chaeta, with Scer\GAL4hs.PB
cone cell | pupal stage | somatic clone
cyst progenitor cell | ectopic, with Scer\GAL4tj.PU
egg chorion (with Rbf11)
egg chorion (with Rbf120a)
embryonic/larval midgut primordium
embryonic epidermis & S phase
embryonic epidermis & S phase | germ-line clone
embryonic epidermis | embryonic stage 13
eye
eye, with Scer\GAL4ey.PH
eye (with Rbf11)
eye (with Rbf120a)
eye | ectopic | somatic clone
eye | somatic clone
eye | somatic clone, with Scer\GAL4Act.PU
eye disc
eye disc, with Scer\GAL4ey.PH
eye disc (with Rbf11)
eye disc (with Rbf120a)
eye disc | somatic clone
eye disc | somatic clone, with Scer\GAL4Act.PU
eye disc | somatic clone, with Scer\GAL4unspecified
eye disc | somatic clone | third instar larval stage
eye disc | third instar larval stage (with Rbf15aΔ)
eye disc | third instar larval stage (with Rbf120-3-3)
eye disc anterior to the morphogenetic furrow | somatic clone
eye disc morphogenetic furrow | somatic clone
eye disc posterior to the morphogenetic furrow | larval stage
eye disc posterior to the morphogenetic furrow | somatic clone | third instar larval stage
eye photoreceptor cell | pupal stage | somatic clone
follicle cell (with Rbf14)
follicle cell (with Rbf120a)
follicle cell & cell cycle (with Rbf11)
follicle cell & cell cycle (with Rbf120a)
hub cell | decreased number, with Scer\GAL4NP1624
interommatidial bristle
interommatidial bristle, with Scer\GAL4GMR.PF
interommatidial bristle | somatic clone
male accessory gland secondary cell, with Scer\GAL4Acp26Aa.PC
male accessory gland secondary cell, with Scer\GAL4esg.PU
mesonotum, with Scer\GAL4pnr-MD237
mitochondrion | spermatogenesis, with Scer\GAL4NP1624
mitotic cell cycle (with Rbf14)
mitotic cell cycle (with Rbf120a)
mitotic cell cycle | somatic clone, with Scer\GAL4Act5C.PP
nucleus | adult stage | decreased number | male, with Scer\GAL4Acp26Aa.PC
nucleus | adult stage | decreased number | male, with Scer\GAL4esg.PU
ommatidium
ommatidium | pupal stage | somatic clone
ommatidium | somatic clone
pigment cell
scutellar bristle
somatic cell of testis, with Scer\GAL4C587
somatic cell of testis, with Scer\GAL4NP1624
somatic cell of testis | somatic clone
somatic cell of testis | somatic clone, with Scer\GAL4Tub.PU
S phase | ectopic
S phase | ectopic & eye disc | posterior (with Rbf11)
S phase | ectopic & eye disc | posterior (with Rbf120a)
submarginal wing cell, with Scer\GAL4dpp.blk1
wing, with Scer\GAL4bbg-C96
wing, with Scer\GAL4Bx-MS1096
wing, with Scer\GAL4da.PU
wing, with Scer\GAL4NP6333
wing, with Scer\GAL4vg.PM
wing blade posterior compartment, with Scer\GAL4en-e16E
wing disc, with Scer\GAL4bbg-C96
wing disc, with Scer\GAL4en.PU
wing disc, with Scer\GAL4ptc.PU
wing disc, with Scer\GAL4vg.PM
wing disc | somatic clone | third instar larval stage
wing disc | somatic clone | wandering third instar larval stage
wing disc | third instar larval stage, with Scer\GAL4ap-md544
wing disc | third instar larval stage, with Scer\GAL4NP6333
wing margin, with Scer\GAL4bbg-C96
wing margin, with Scer\GAL4NP6333
wing margin, with Scer\GAL4vg.PM
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (3)
Hsap\RBL2
14 of 14
Yes
Yes
Hsap\RBL1
13 of 14
No
Yes
Hsap\RB1
6 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (3)
Rnor\Rbl2
14 of 14
Yes
Yes
Rnor\Rbl1
13 of 14
No
Yes
Rnor\Rb1
6 of 14
No
Yes
Mus musculus (laboratory mouse) (3)
Mmus\Rbl2
14 of 14
Yes
Yes
Mmus\Rbl1
13 of 14
No
Yes
Mmus\Rb1
6 of 14
No
Yes
1  
Xenopus tropicalis (Western clawed frog) (3)
Xtro\rbl2
8 of 13
Yes
Yes
Xtro\rbl1
7 of 13
No
Yes
Xtro\rb1
2 of 13
No
Yes
Danio rerio (Zebrafish) (3)
Drer\rbl2
14 of 14
Yes
Yes
Drer\rbl1
13 of 14
No
Yes
Drer\rb1
6 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\lin-35
13 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP006477
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\RBR1
10 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Rbf. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
Rbf2
10 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 4 )
Potential Models Based on Orthology ( 5 )
Modifiers Based on Experimental Evidence ( 5 )
Allele
Disease
Interaction
References
RbfUAS.cDa
ameliorates  Parkinson's disease
modeled by LrrkI1915T.UAS
(Gehrke et al., 2010)
ameliorates  brain glioma
modeled by btl::Egfrλ.UAS, Pi3K92EUAS.Tag:MYC,Tag:PM(hKRAS)
(Read et al., 2009)
ameliorates  tauopathy
modeled by dapUAS.cdNa, Hsap\MAPTR406W.UAS
(Khurana et al., 2006)
RbfGD4484
exacerbates  brain glioma
modeled by btl::Egfrλ.UAS, Pi3K92EUAS.Tag:MYC,Tag:PM(hKRAS)
(Read et al., 2009)
exacerbates  cancer
modeled by MycUAS.cZa, p53RNAi.UAS.RasG12D
(Diaz et al., 2024)
RbfGMR.PU
ameliorates  viral infectious disease
modeled by EBV\BRLF1GMR.PA
(Adamson and Lajeunesse, 2012)
ameliorates  infectious mononucleosis
modeled by EBV\BRLF1GMR.PA
(Adamson and Lajeunesse, 2012)
ameliorates  Epstein-Barr virus hepatitis
modeled by EBV\BRLF1GMR.PA
(Adamson and Lajeunesse, 2012)
Rbf15aΔ
model of  cancer
is ameliorated by psidin55D4
(Sheng and Du, 2020)
is ameliorated by psidin85D1
(Sheng and Du, 2020)
is ameliorated by psidinAE
(Sheng and Du, 2020)
is ameliorated by psidinCJ
(Sheng and Du, 2020)
RbfHMS03004
model of  cancer
is exacerbated by α-SpecHMC04371
(Parkhitko et al., 2021)
is exacerbated by Ada2aHMJ03126
(Parkhitko et al., 2021)
is exacerbated by Blos2HMJ21554
(Parkhitko et al., 2021)
is exacerbated by CG10600HMS05767
(Parkhitko et al., 2021)
is exacerbated by CG16903HMC06024
(Parkhitko et al., 2021)
is exacerbated by CG31871HMS05379
(Parkhitko et al., 2021)
is exacerbated by Cdc5HMC04732
(Parkhitko et al., 2021)
is exacerbated by Cse1JF02972
(Parkhitko et al., 2021)
is exacerbated by CG6015HMS01037
(Parkhitko et al., 2021)
is exacerbated by NkapHMS00995
(Parkhitko et al., 2021)
is exacerbated by Cul3HMS01572
(Parkhitko et al., 2021)
is exacerbated by Cul4HMC02981
(Parkhitko et al., 2021)
is exacerbated by CG7483HMS00696
(Parkhitko et al., 2021)
is exacerbated by Cdc27HM04024
(Parkhitko et al., 2021)
is exacerbated by SmFJF02276
(Parkhitko et al., 2021)
is exacerbated by CycAJF02472
(Parkhitko et al., 2021)
is exacerbated by Taf1HMS00416
(Parkhitko et al., 2021)
is exacerbated by Dim1HMC03851
(Parkhitko et al., 2021)
is exacerbated by Dis3HMJ24000
(Parkhitko et al., 2021)
is exacerbated by Dlg5HMJ23170
(Parkhitko et al., 2021)
is exacerbated by E(Pc)JF03101
(Parkhitko et al., 2021)
is exacerbated by HDAC1JF01401
(Parkhitko et al., 2021)
is exacerbated by Hsc70-1HMS00888
(Parkhitko et al., 2021)
is exacerbated by Pp2A-29BJF03316
(Parkhitko et al., 2021)
is exacerbated by Pp4-19CHMJ21831
(Parkhitko et al., 2021)
is exacerbated by Prosα7HMS00068
(Parkhitko et al., 2021)
is exacerbated by Tango1HMS05801
(Parkhitko et al., 2021)
is exacerbated by RanHMS02885
(Parkhitko et al., 2021)
is exacerbated by Hsc70-4HMS00152
(Parkhitko et al., 2021)
is exacerbated by KCNQJF02562
(Parkhitko et al., 2021)
is exacerbated by Rm62JF01385
(Parkhitko et al., 2021)
is exacerbated by TlkHMS00943
(Parkhitko et al., 2021)
is exacerbated by RpS29HMJ21541
(Parkhitko et al., 2021)
is exacerbated by Kdm5HM05155
(Parkhitko et al., 2021)
is exacerbated by U2af38HMS04505
(Parkhitko et al., 2021)
is exacerbated by Mfap1HM05216
(Parkhitko et al., 2021)
is exacerbated by RpS4HMS05869
(Parkhitko et al., 2021)
is exacerbated by U2af50HMC03810
(Parkhitko et al., 2021)
is exacerbated by Rpn8HMS05743
(Parkhitko et al., 2021)
is exacerbated by Mi-2HMS00301
(Parkhitko et al., 2021)
is exacerbated by Nipped-BHM05172
(Parkhitko et al., 2021)
is exacerbated by U4-U6-60KHMC03931
(Parkhitko et al., 2021)
is exacerbated by Non1JF01590
(Parkhitko et al., 2021)
is exacerbated by Rpn9HMS01007
(Parkhitko et al., 2021)
is exacerbated by SceHMS05745
(Parkhitko et al., 2021)
is exacerbated by Not1HMS00526
(Parkhitko et al., 2021)
is exacerbated by Uba1JF01977
(Parkhitko et al., 2021)
is exacerbated by Pgam1HMC04818
(Parkhitko et al., 2021)
is exacerbated by Pp1-87BHMS00409
(Parkhitko et al., 2021)
is exacerbated by Sec31HMS00666
(Parkhitko et al., 2021)
is exacerbated by SkpAHMS00791
(Parkhitko et al., 2021)
is exacerbated by Snap29JF01883
(Parkhitko et al., 2021)
is exacerbated by snRNP-U1-70KHMS00274
(Parkhitko et al., 2021)
is exacerbated by snRNP-U1-CHMC04045
(Parkhitko et al., 2021)
is exacerbated by Ubc6HMS02466
(Parkhitko et al., 2021)
is exacerbated by sqhHMS00437
(Parkhitko et al., 2021)
is exacerbated by Spt6HMS00364
(Parkhitko et al., 2021)
is exacerbated by VhaAC39-1HMS01442
(Parkhitko et al., 2021)
is exacerbated by Su(H)HMS05748
(Parkhitko et al., 2021)
is exacerbated by Su(var)2-10HMS00750
(Parkhitko et al., 2021)
is exacerbated by svJF02582
(Parkhitko et al., 2021)
is exacerbated by trrHMS01019
(Parkhitko et al., 2021)
is exacerbated by domHMS02208
(Parkhitko et al., 2021)
is exacerbated by trxHMS00580
(Parkhitko et al., 2021)
is exacerbated by SumoJF02869
(Parkhitko et al., 2021)
is exacerbated by eIF3mHMS00667
(Parkhitko et al., 2021)
is exacerbated by eIF4G1HMS00762
(Parkhitko et al., 2021)
is exacerbated by tumHMS01417
(Parkhitko et al., 2021)
is exacerbated by gppJF01283
(Parkhitko et al., 2021)
is exacerbated by waplHMJ22638
(Parkhitko et al., 2021)
is exacerbated by wupAJF02172
(Parkhitko et al., 2021)
is exacerbated by jubHMS02335
(Parkhitko et al., 2021)
is exacerbated by kin17HMC03906
(Parkhitko et al., 2021)
is exacerbated by ktoHMS01062
(Parkhitko et al., 2021)
is exacerbated by l(1)10BbHMS00255
(Parkhitko et al., 2021)
is exacerbated by lqfHMJ22079
(Parkhitko et al., 2021)
is exacerbated by mRNA-capHMS04494
(Parkhitko et al., 2021)
is exacerbated by peaHMS00528
(Parkhitko et al., 2021)
is exacerbated by pydHMS00263
(Parkhitko et al., 2021)
is exacerbated by qkr58E-2HMC03966
(Parkhitko et al., 2021)
is exacerbated by sktlJF02796
(Parkhitko et al., 2021)
is ameliorated by Naa20AGL01093
(Sheng and Du, 2020)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
RBL2; RB transcriptional corepressor like 2
14 of 14
180203
RBL1; RB transcriptional corepressor like 1
13 of 14
116957
      RB1; RB transcriptional corepressor 1
      6 of 14
      614041
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Rbf - CG8089
      two hybrid array
      (Shokri et al., 2019)
      Rbf - Caf1-55
      anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, coimmunoprecipitation
      (Lee et al., 2012, Korenjak et al., 2004, Taylor-Harding et al., 2004)
      Rbf - Cap-D3
      anti tag coimmunoprecipitation, western blot
      (Longworth et al., 2008)
      Rbf - Cp190
      anti bait coimmunoprecipitation, western blot
      (Korenjak et al., 2014)
      Rbf - CrebB
      two hybrid array
      (Shokri et al., 2019)
      Rbf - Dp
      anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based, anti tag western blot
      (Rhee et al., 2014, Shibutani et al., 2007, Korenjak et al., 2004, Taylor-Harding et al., 2004, Weng et al., 2003, Du et al., 1996)
      Rbf - E(z)
      anti bait coimmunoprecipitation, anti tag western blot
      (Lee et al., 2010)
      Rbf - E2f1
      anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based, coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, anti tag western blot, pull down, two hybrid, autoradiography
      (Kim et al., 2018, Rhee et al., 2014, Ran et al., 2013, Davidson and Duronio, 2012, Acharya et al., 2010, Ahlander et al., 2008, Lu et al., 2007, Shibutani et al., 2007, Taylor-Harding et al., 2004, Weng et al., 2003, Du et al., 1996)
      Rbf - E2f2
      anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based, anti tag western blot, pull down, autoradiography
      (Rhee et al., 2014, Lee et al., 2012, Nicolay et al., 2011, Acharya et al., 2010, Korenjak et al., 2004, Taylor-Harding et al., 2004)
      Rbf - HDAC1
      anti tag coimmunoprecipitation, western blot
      (Taylor-Harding et al., 2004)
      Rbf - Myb
      anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot
      (Korenjak et al., 2004, Lewis et al., 2004)
      Rbf - Orc1
      pull down, western blot
      (Bosco et al., 2001)
      Rbf - Orc2
      anti tag coimmunoprecipitation, anti tag western blot, fluorescent resonance energy transfer, fluorescence microscopy, pull down, western blot
      (Ahlander et al., 2008, Bosco et al., 2001)
      Rbf - Trl
      anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot
      (Bayarmagnai et al., 2012)
      Rbf - Ubi-p5E
      anti tag coimmunoprecipitation, anti tag western blot
      (Raj et al., 2012)
      Rbf - Ubi-p63E
      anti tag coimmunoprecipitation, anti tag western blot
      (Raj et al., 2012)
      Rbf - bun
      two hybrid array
      (Shokri et al., 2019)
      Rbf - ebi
      anti tag coimmunoprecipitation, anti tag western blot
      (Lim et al., 2013)
      Rbf - fkh
      two hybrid array
      (Shokri et al., 2019)
      Rbf - glu
      anti tag coimmunoprecipitation, western blot
      (Longworth et al., 2008)
      Rbf - lin-52
      anti tag coimmunoprecipitation, western blot
      (Lewis et al., 2012)
      Rbf - mip120
      anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot
      (Korenjak et al., 2004, Lewis et al., 2004)
      Rbf - mip130
      anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot
      (Korenjak et al., 2004, Lewis et al., 2004)
      Rbf - mip40
      anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot, affinity chromatography technology
      (Beall et al., 2007, Korenjak et al., 2004)
      Rbf - sqd
      anti tag coimmunoprecipitation, multidimensional protein identification technology, western blot, anti bait coimmunoprecipitation
      (Ahlander and Bosco, 2009)
      Rbf - twi
      two hybrid array
      (Shokri et al., 2019)
      Rbf - yki
      anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation
      (Rader et al., 2023)
      Summary of Genetic Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Rbf
      enhanceable
      Apc
      (Zhang et al., 2014)
      Rbf
      enhanceable
      arm
      (Zhang et al., 2014)
      Rbf
      suppressible
      Atg1
      (Kwon et al., 2013)
      Rbf
      enhanceable
      Axn
      (Zhang et al., 2014)
      Rbf
      suppressible
      bsk
      (Milet et al., 2014)
      Rbf
      enhanceable
      Cbl
      (Truscott et al., 2014)
      Rbf
      suppressible
      Cbl
      (Truscott et al., 2014)
      Rbf
      suppressible
      Cdk4
      (Datar et al., 2000)
      Rbf
      suppressible
      CycD
      (Datar et al., 2006, Xin et al., 2002, Datar et al., 2000)
      Rbf
      suppressible
      CycD|Cdk4
      (Datar et al., 2000)
      Rbf
      enhanceable
      CycE
      (Kwon et al., 2013, Secombe et al., 1998)
      Rbf
      enhanceable
      dap
      (Weng et al., 2003, Xin et al., 2002)
      Rbf
      suppressible
      dap
      (Xin et al., 2002)
      rno|Rbf
      enhanceable
      Delta
      (Steele et al., 2009)
      rno|Rbf
      suppressible
      Delta
      (Steele et al., 2009)
      Rbf
      enhanceable
      dom
      (Kwon et al., 2013)
      Rbf
      suppressible
      Dp
      (Sainz de la Maza et al., 2022, Moon et al., 2008)
      Rbf
      suppressible
      Dp|E2f1
      (Kuzin et al., 2000)
      gig|Rbf
      suppressible
      Dronc
      (Li et al., 2010)
      Rbf|gig
      suppressible
      Dronc
      (Li et al., 2010)
      rno|Rbf
      suppressible
      Dronc
      (Steele et al., 2009)
      Rbf|Stam
      suppressible
      E2f1
      (Sheng et al., 2016)
      Rbf
      suppressible
      E2f1
      (Sainz de la Maza et al., 2022, Kwon et al., 2013, Weng et al., 2003, Du, 2000)
      rno|Rbf
      suppressible
      E2f1
      (Steele et al., 2009)
      Rbf|gig
      suppressible
      E2f1
      (Li et al., 2010)
      dap|Rbf
      suppressible
      E2f1
      (Weng et al., 2003)
      Rbf|dap
      suppressible
      E2f1
      (Weng et al., 2003)
      Rbf|Tsc1
      suppressible
      E2f1
      (Hsieh et al., 2010)
      gig|Rbf
      suppressible
      E2f1
      (Li et al., 2010)
      Rbf
      enhanceable
      EcR
      (Kwon et al., 2013)
      Rbf|Stam
      suppressible
      Egfr
      (Sheng et al., 2016)
      Rbf|psidin
      suppressible
      Egfr
      (Sheng and Du, 2020)
      Rbf
      suppressible
      Ets97D
      (Sainz de la Maza et al., 2022)
      Rbf
      suppressible
      Ets97D|srl
      (Sainz de la Maza et al., 2022)
      Rbf
      suppressible
      hep
      (Milet et al., 2014)
      Axn|Rbf
      suppressible
      hid
      (Zhang et al., 2014)
      Rbf
      suppressible
      hid
      (Tanaka-Matakatsu et al., 2009)
      Rbf|gig
      suppressible
      hid
      (Li et al., 2010)
      Rbf|Stam
      suppressible
      hid
      (Sheng et al., 2016)
      gig|Rbf
      suppressible
      hid
      (Li et al., 2010)
      Rbf
      enhanceable
      Hrs
      (Sheng et al., 2016)
      Rbf|yki
      suppressible
      hth
      (Rader et al., 2023)
      Rbf
      enhanceable
      lilli
      (Kwon et al., 2013)
      Rbf|Axn
      enhanceable
      Lkb1
      (Zhang et al., 2014)
      Rbf
      enhanceable
      lola
      (Kwon et al., 2013)
      Rbf
      enhanceable
      mam
      (Kwon et al., 2013)
      Rbf|Cbl
      suppressible
      mir-998
      (Truscott et al., 2014)
      Rbf
      enhanceable
      mir-998
      (Truscott et al., 2014)
      Rbf
      suppressible
      mir-998
      (Truscott et al., 2014)
      Rbf
      enhanceable
      mir-ban
      (Tanaka-Matakatsu et al., 2009)
      Rbf
      enhanceable
      N
      (Kwon et al., 2013)
      Rbf
      suppressible
      N
      (Kwon et al., 2013)
      Rbf
      enhanceable
      Naa20A
      (Sheng and Du, 2020)
      Rbf
      suppressible
      Nos
      (Stasiv et al., 2004)
      Rbf
      suppressible
      p53
      (Moon et al., 2008)
      Rbf
      enhanceable
      Pabp2
      (Kwon et al., 2013)
      Rbf
      enhanceable
      psidin
      (Sheng and Du, 2020)
      Rbf
      suppressible
      ptc
      (Duman-Scheel et al., 2002)
      Rbf
      enhanceable
      pum
      (Kwon et al., 2013)
      Rbf|psidin
      suppressible
      Raf
      (Sheng and Du, 2020)
      Axn|Rbf
      suppressible
      raptor
      (Zhang et al., 2014)
      Rbf|psidin
      suppressible
      Ras85D
      (Sheng and Du, 2020)
      Rbf|Stam
      suppressible
      Ras85D
      (Sheng et al., 2016)
      Rbf|Tsc1
      suppressible
      Rheb
      (Hsieh et al., 2010)
      Axn|Rbf
      suppressible
      Rheb
      (Zhang et al., 2014)
      Rbf
      suppressible
      Rheb
      (Hsieh et al., 2010)
      Rbf
      suppressible
      rho
      (Sheng et al., 2016)
      Rbf
      suppressible
      rpr
      (Moon et al., 2008)
      Rbf
      suppressible
      S6k
      (Hsieh et al., 2010)
      gig|Rbf
      suppressible
      S6k
      (Li et al., 2010)
      Rbf|gig
      suppressible
      S6k
      (Hsieh et al., 2010, Li et al., 2010)
      Rbf
      enhanceable
      sgg
      (Zhang et al., 2014)
      Rbf|Stam
      suppressible
      spi
      (Sheng et al., 2016)
      Rbf
      enhanceable
      sqd
      (Ahlander and Bosco, 2009)
      Rbf
      suppressible
      srl
      (Sainz de la Maza et al., 2022)
      Rbf
      enhanceable
      Stam
      (Sheng et al., 2016)
      Rbf
      suppressible
      tara
      (Kwon et al., 2013)
      Rbf
      enhanceable
      Tsc1
      (Hsieh et al., 2010)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Axn
      enhanceable
      Rbf
      (Zhang et al., 2014)
      btl::Egfr|Pi3K92E
      enhanceable
      Rbf
      (Read et al., 2009)
      Cdk4
      suppressible
      Rbf
      (Meyer et al., 2000)
      CycD|Cdk4
      suppressible
      Rbf
      (Xin et al., 2002)
      CycE
      enhanceable
      Rbf
      (Secombe et al., 1998)
      Delta|Utx
      suppressible
      Rbf
      (Herz et al., 2010)
      Delta|lola|psq
      suppressible
      Rbf
      (Ferres-Marco et al., 2006)
      Dp|E2f1
      enhanceable
      Rbf
      (Du and Dyson, 1999)
      E2f1
      suppressible
      Rbf
      (Moon et al., 2008, Morris et al., 2006, Du, 2000)
      E2f1|Dp
      enhanceable
      Rbf
      (Du and Dyson, 1999)
      EcR
      suppressible
      Rbf
      (Sekar et al., 2023)
      gig
      enhanceable
      Rbf
      (Hsieh et al., 2010)
      hid|Kdm5
      enhanceable
      Rbf
      (Herz and Bergmann, 2024)
      hid|Myc
      enhanceable
      Rbf
      (Herz and Bergmann, 2024)
      Hrs
      enhanceable
      Rbf
      (Sheng et al., 2016)
      lin-52
      enhanceable
      Rbf
      (Bhaskar et al., 2014)
      lola|psq|Delta
      enhanceable
      Rbf
      (Ferres-Marco et al., 2006)
      Lrrk
      suppressible
      Rbf
      (Gehrke et al., 2010)
      Nsf2
      suppressible
      Rbf
      (Laviolette et al., 2005)
      pan
      suppressible
      Rbf
      (Duman-Scheel et al., 2004)
      Phb1
      suppressible
      Rbf
      (Zhao et al., 2022)
      Phb2
      suppressible
      Rbf
      (Zhao et al., 2022)
      Pi3K92E|btl::Egfr
      suppressible
      Rbf
      (Read et al., 2009)
      Rheb
      suppressible
      Rbf|dap
      (Khurana et al., 2006)
      rno
      enhanceable
      Rbf
      (Steele et al., 2009)
      sqd
      enhanceable
      Rbf
      (Ahlander and Bosco, 2009)
      Stam
      enhanceable
      Rbf
      (Sheng et al., 2016)
      tkv
      suppressible
      Rbf
      (Sekar et al., 2023, Martin-Castellanos and Edgar, 2002)
      Tsc1
      enhanceable
      E2f1|Rbf
      (Hsieh et al., 2010)
      Tsc1
      enhanceable
      Rbf
      (Hsieh et al., 2010)
      Utx
      enhanceable
      Rbf
      (Herz et al., 2010)
      wg
      suppressible
      Rbf
      (Ewen-Campen and Perrimon, 2024)
      External Data
      Subunit Structure (UniProtKB)
      Forms a complex with the DRTF1/E2F transcription factor through binding to a C-terminal region of E2F. This binding inhibits the E2F-mediated transactivation activity. Component of the DREAM complex at least composed of Myb, Caf1-55, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, HDAC1/Rpd3 and l(3)mbt.
      (UniProt, Q24472 )
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      External Data
      Linkouts
      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      X
      Recombination map
      1-0
      Cytogenetic map
      1C5-1C5
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      1C5-1C5
      Limits computationally determined from genome sequence between P{EP}sdkEP369 and P{EP}EP1600&P{EP}EP1498
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      1C-1D
      (determined by in situ hybridisation)
      (Du and Dyson, 1999)
      Experimentally Determined Recombination Data
      Location

      1-0

      (FlyBase, 2016)

      1-0.0

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (32)
      Genomic Clones (7)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (216)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Clavier et al., 2014, Milet et al., 2014, Acharya et al., 2012, Du et al., 1996, Dougan and DiNardo, 1992)
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         

        • New stable cell line derived from S2-unspecified : S2 cell lines stably expressing either Tag:FLAG-tagged Rbf wild-type (R1WT) or Rbf mutant (R1A or R1E) proteins under the inducible metallothionein promoter were generated.

          (Lee et al., 2012)
        Other Comments

        gig and Rbf synergistically induce oxidative stress via increased protein synthesis, inhibited de novo lipid synthesis, and decreased reactive oxygen species enzyme induction.

        (Li et al., 2010)

        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

        (Kiger et al., 2003)

        E2f2 as part of the E2f-Rb complexes has a role in the down regulation of CycE during the gap phase of endocycling cells in normal development.

        (Weng et al., 2003)

        Rbf is required at multiple stages of development. Most, if not all, of the function of Rbf during development is mediated through E2f.

        (Du, 2000)

        Rbf is dispensable for early cell cycles in the embryo, but plays an essential role in the introduction of G1 control during development.

        (Du and Dyson, 1999)

        Identification: Yeast two hybrid screen for proteins that interact with E2f.

        (Du et al., 1996)

        Rbf plays a critical role in the regulation of cell proliferation.

        (Du et al., 1996)

        Overexpression of dap during eye development interferes with cell cycle progression and interacts genetically with Rbf and CycE.

        (de Nooij et al., 1996)
        Relationship to Other Genes
        Source for database merge of
        Additional comments
        Nomenclature History
        Source for database identify of

        Source for identity of: Rbf CG7413

        (Nusskern, 1999.11)
        Nomenclature comments
        Etymology
        Synonyms and Secondary IDs (25)
        Reported As
        Symbol Synonym
        CG7413
        (Meadows, 2017.1.11, Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Pan and Zhang, 2009, Perkins et al., 2009, Beall et al., 2007, Laviolette et al., 2005, Benos, 2000.12.13)
        EG:34F3.3
        (Benos et al., 2001, Benos, 2000.12.13)
        FBF
        (Bassett et al., 1997)
        RB
        (Gordon et al., 2013, Korenjak et al., 2012, Kondo and Perrimon, 2011)
        RBF
        (Liu et al., 2024, Bhaskar et al., 2014, Milet et al., 2014, Nicolay et al., 2013, Milet et al., 2010, Swaminathan et al., 2010, Steele et al., 2009, Wen et al., 2008, Wang et al., 2006, Baonza and Freeman, 2005, Moon et al., 2004, Frolov et al., 2003, Weng et al., 2003, Cayirlioglu and Duronio, 2001, Prober and Edgar, 2001, Kuzin et al., 2000, Li and Vaessin, 2000, Meyer et al., 2000, Prober and Edgar, 2000, Du, 1999, Duronio, 1999, Huen et al., 1998, Neufeld and Edgar, 1998, Neufeld et al., 1998, Neufeld et al., 1998, Secombe et al., 1998, Secombe et al., 1998, Du, 1996.3.22)
        RBF1
        (Kim et al., 2018, Elenbaas et al., 2015, Korenjak et al., 2014, Ran et al., 2013, Korenjak et al., 2012, Lee et al., 2012, Lewis et al., 2012, Lee et al., 2010, Ambrus et al., 2009, Murawska et al., 2008, Nicolay and Frolov, 2008, Wen et al., 2008, Ambrus et al., 2007, Georlette et al., 2007, Herr et al., 2007, Tountas and Fortini, 2007, Legent et al., 2006, Baonza and Freeman, 2005, Korenjak and Brehm, 2005, Korenjak et al., 2004, Lewis et al., 2004, Lipsick, 2004, Taylor-Harding et al., 2004, Frolov et al., 2003, Douglas et al., 2002, Stevaux et al., 2002, Douglas et al., 2000, Harbour and Dean, 2000)
        RBf1
        (Wei et al., 2015)
        Rb
        (Chatterjee and Deng, 2019, Grewal, 2015, Bonke et al., 2013, Kohzaki and Murakami, 2005, Tower, 2004, McNairn and Gilbert, 2003, Silver and Montell, 2003, Du et al., 2002, Campuzano, 2001, Duman-Scheel et al., 2001, Oldham et al., 2000, Steller, 2000, Weinkove and Leevers, 2000, Enikolopov et al., 1999)
        Rb1
        (Rubin et al., 2000)
        RbF
        (Saucedo et al., 2001)
        RbF1
        (Almeida Machado Costa et al., 2022)
        Rbf
        (Aughey et al., 2025, Martinez, 2025, Collins et al., 2024, Ewen-Campen and Perrimon, 2024, Li et al., 2024, Li et al., 2024, Raicu et al., 2024, Zappia et al., 2023, Herrera et al., 2021, Morin-Poulard et al., 2021, Graves et al., 2020, Hinnant et al., 2020, Li et al., 2020, Luo et al., 2020, Rust et al., 2020, Sheng and Du, 2020, Murcia et al., 2019, Ramanathan et al., 2019, Shokri et al., 2019, van den Ameele and Brand, 2019, Akishina et al., 2018, Ariss et al., 2018, Bischof et al., 2018, Gene Disruption Project members, 2018-, Lee et al., 2018, Song et al., 2018, Tamirisa et al., 2018, Akishina et al., 2017, Romero-Pozuelo et al., 2017, Transgenic RNAi Project members, 2017-, Zhang et al., 2017, Dominado et al., 2016, Yadav et al., 2016, Bouleau and Tricoire, 2015, Doggett et al., 2015, Haelterman, 2015.3.20, Schertel et al., 2015, Sreedharan et al., 2015, Tokusumi et al., 2015, Wang and Baker, 2015, Lee et al., 2014, Liang et al., 2014, Rhee et al., 2014, Tipping and Perrimon, 2014, Truscott et al., 2014, Bischof et al., 2013, Bonke et al., 2013, Das et al., 2013, Kwon et al., 2013, Lim et al., 2013, Pomerantz and Blau, 2013, Schertel et al., 2013, Yamamoto et al., 2013-, Adamson and Lajeunesse, 2012, Japanese National Institute of Genetics, 2012.5.21, Zhai et al., 2012, Axelsson et al., 2011, Gordon and Du, 2011, Klusza and Deng, 2011, Miles et al., 2011, Toku et al., 2011, Herranz et al., 2010, Herz et al., 2010, Lloyd and Taylor, 2010, Nedelsky et al., 2010, Popodi et al., 2010-, Swaminathan et al., 2010, Venken et al., 2010, Wasbrough et al., 2010, Ahlander and Bosco, 2009, Diangelo and Birnbaum, 2009, Jiang et al., 2009, Metzendorf et al., 2009, Ogura et al., 2009, Steele et al., 2009, Suzuki et al., 2009, Oktaba et al., 2008, Doggett et al., 2007, Ringrose and Paro, 2007, Straub and Becker, 2007, Tadros et al., 2007, Axelson, 2006, Morris et al., 2006, Laviolette et al., 2005, Thomas, 2005)
        Rbf1
        (Hoareau et al., 2024, Li et al., 2024, Raicu et al., 2024, Trujillo et al., 2024, Payankaulam et al., 2022, Parkhitko et al., 2021, Rajasekaran et al., 2021, Mouawad et al., 2020, Mouawad et al., 2019, Wu et al., 2019, Elenbaas et al., 2015, Spratford and Kumar, 2015, Miles et al., 2014, Singer et al., 2014, Zhang et al., 2014, Davidson and Duronio, 2012, Ji et al., 2012, Miles et al., 2012, Acharya et al., 2010, Hsieh et al., 2010, Manning et al., 2010, Maqbool et al., 2010, Read et al., 2009, Ahlander et al., 2008, Davidson et al., 2008, Shibutani et al., 2008, Wei et al., 2008, Beall et al., 2007, Buckley et al., 2007, Garcia et al., 2007, Lu et al., 2007, Shibutani et al., 2007, Shibutani et al., 2007, Steinhilb et al., 2007, Swanhart et al., 2007, Ullah et al., 2007, Buckley et al., 2006, Buttitta and Edgar, 2006, Datar et al., 2006, Khurana et al., 2006, Sun and Deng, 2006, Keller et al., 2005, Kohzaki and Murakami, 2005, Shibutani et al., 2005, Stevaux et al., 2005, Swanhart et al., 2004, Cayirlioglu et al., 2003, Edgar et al., 2003, Frolov et al., 2003, Key et al., 2003, Brumby et al., 2002)
        dRBF
        (Coelho and Leevers, 2000, Secombe et al., 1997)
        pRb
        (Miles et al., 2014, Szawinski et al., 2005)
        rb
        (Roy and Ingham, 2002)
        rbf
        (Church et al., 2017, Sheng et al., 2016, Truscott et al., 2016, Zhang et al., 2014, Gordon et al., 2013, Acharya et al., 2010, Buttitta et al., 2010, Gehrke et al., 2010, Li et al., 2010, Nicolay et al., 2010, Martinez et al., 2009, Steele et al., 2009, Tanaka-Matakatsu et al., 2009, Escudero and Freeman, 2007, Hartl et al., 2007, Steele et al., 2007, Tanaka-Matakatsu et al., 2007, Bjorklund et al., 2006, Brun et al., 2006, Foglietti et al., 2006, Firth and Baker, 2005, Moon et al., 2005, Moon et al., 2004, Du, 2000, Du and Dyson, 1999)
        rbf1
        (Church et al., 2017, Bradley-Gill et al., 2016, Truscott et al., 2014, Collins and Moon, 2013, Krivy et al., 2013, Nicolay et al., 2013, Korenjak et al., 2012, Longworth et al., 2012, Popova et al., 2011, Gilbert et al., 2009, Nicholson et al., 2009, Longworth et al., 2008, Moon et al., 2008, Buttitta et al., 2007, Moon et al., 2007, Ullah et al., 2007, Moon et al., 2006, Stevaux et al., 2005, Dimova et al., 2003, Lee and Orr-Weaver, 2003)
        Name Synonyms
        Rb
        (Steele et al., 2009)
        Retinoblastoma
        (Miles et al., 2011, Herz et al., 2010)
        Retinoblastoma-family protein
        (Graves et al., 2020, Lee et al., 2014, Swaminathan et al., 2010, Tanaka-Matakatsu et al., 2009, Cho et al., 2005, Du and Dyson, 1996)
        retinoblastoma
        (Axelson, 2006)
        retinoblastoma factor
        (Escudero and Freeman, 2007)
        retinoblastoma factor-1
        (Steinhilb et al., 2007)
        retinoblastoma-family homolog
        (Diangelo and Birnbaum, 2009)
        retinoblastoma-family protein
        (Martinez et al., 2009)
        Secondary FlyBase IDs
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 49 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          FlyMine - An integrated database for Drosophila genomics
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          MIST (genetic) - An integrated Molecular Interaction Database
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          References (375)